Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 69  |  Page : 264-273

Bioassay-guided isolation, identification, and evaluation of anti-inflammatory activity of β-boswellic alcohol and 3-o-acetyl-11-hydroxy-β-boswellic acid from the leaves of Boswellia serrata


1 Department of Pharmaceutical Chemistry, Bhagwant University, Ajmer, Rajasthan, India
2 Trivedi Science Research Laboratory, Thane, Maharashtra, India

Correspondence Address:
Snehasis Jana
Trivedi Science Research Laboratory, Thane-West, Maharashtra
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_17_20

Rights and Permissions

Background: There are no in-depth studies on the extracts of the leaves of Boswellia serrata (BS), which contain many bioactive phytoconstituents and responsible for their anti-inflammatory effects. Objectives: The goal of this research plan was the isolation and purification of most active compounds from the leaves of BS via bioassay-guided fractionation based on anti-inflammatory activity. Materials and Methods: The silica gel column chromatographic techniques with different solvent systems were used for the separation of the constituents of the ethyl acetate-soluble fraction of BS leaves. The structures of the isolated compounds were assigned based on various spectroscopic analysis (high-resolution mass spectrometry,[1]H NMR, and[13]C NMR) and comparison with literature data. The anti-inflammatory activity of all crude extracts, subfractions of ethyl acetate extract, and two isolated new compounds were evaluated in cell-free (cyclooxygenase and 5-lipoxygenase) and cell-based assays (nitrite and tumor necrosis factor-alpha) in RAW264.7. Results: Bioassay-guided fractionation of the extracts of the leaves of BS afforded two ursane-type triterpenoids (compound 5, β-boswellic alcohol, and compound 6, 3-O-acetyl-11-hydroxy-β-boswellic acid) and other ten known triterpene acids. Compound 5 and 6 exhibited strong anti-inflammatory activity with an IC50value of a range 26.5–34.2 μM and 18.3–21.1 μM, respectively, in cell-free and cell-based assays. Conclusion: This is the first report on isolation and identification of β-boswellic alcohol and 3-O-acetyl-11-hydroxy-β-boswellic acid from the leaves of BS, which showed strong anti-inflammatory activity and holds great promise for the treatment of numerous inflammatory diseases.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed66    
    Printed3    
    Emailed0    
    PDF Downloaded22    
    Comments [Add]    

Recommend this journal