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ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 69  |  Page : 227-234

Extraction, isolation and identification of kaempferol 3,7 – Diglucoside in the leaf extracts of Evolvulus alsinoides (Linn.) and its inhibition potency against α-amylase, α-glucosidase, Acetylcholinesterase and amyloid aggregation


Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode, Tamil Nadu, India

Correspondence Address:
Pavithra Mettupalayam Kaliyannan Sundaramoorthy
Assistant Professor (Sr.G), Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_583_19

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Background: Evolvulus alsinoides (Linn.) is an eminent kaphahara (balancing kapha ) plant in Ayurvedic medicine commonly used as brain tonic. Objectives: To report the enzyme inhibitory potency of the plant extract and to isolate the compound of significant impact. Materials and Methods: The plant extracts were obtained by serial exhaustive extraction and successive chromatographic separation. Antioxidant and enzyme inhibition assays were carried out to extract the required isolate. Results: The current research identifies for the first time, the presence of Kaempferol 3,7 diglycoside in the leaf extracts by Spectrophotometry, Chromatography,[1]H and[13]C Nuclear Magnetic Resonance, carboxylic and hydroxylic hydrolysis. The isolated and characterized compound possesses enzyme inhibition property towards α-amylase, α-glucosidase and Acetylcholinesterase . Conclusion: The compound protects differentiating neuronal cells, SH-SY5Y from Amyloid β Peptide-induced Injury. Thus, the newly identified compound could serve as a plant-based bioactive in the management of Alzheimer's.


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