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ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 68  |  Page : 224-228

Antioxidant activity and enhanced cytotoxicity of aqueous Mucuna pruriens L. leaf extract by doxorubicin on different human cancer cell lines


1 Department for Applied and Engineering Chemistry, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
2 Department of Pharmacology, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, England, UK
3 Department of Physiology, College of Health Sciences, Nile University of Nigeria, Abuja, Nigeria
4 Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
5 Faculty of Medicine, University of Novi Sad, Novi Sad; Department for Science, Research and Education, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia

Correspondence Address:
Oke-Oghene Philomena Akpoveso
School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, England
UK
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_413_19

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Background: Conventional cancer drugs have the disadvantage of severe side effects and resistance. Therefore, research targeted toward developing novel therapeutic strategies is needed. Mucuna pruriens (MP) leaf extracts have been suggested to be useful for the management of several diseases including cancer. Objective: The aim of this study was to evaluate the antioxidant and cytotoxic effect of an aqueous leaf extract of MP in different human cancer cell lines. This study also evaluated the enhanced cytotoxic effect of an aqueous leaf extract of MP with Doxorubicin (Dox) in the selected human cancer cell lines. Materials and Methods: In this study, the breast cancer cell lines (MCF-7 and MDA-MB-231), cervix carcinoma cell line (HeLa), and colon cancer cell line (HT-29) were used. As a control, the non-cancer lung cell line (MRC-5) was used. Cytotoxicity was assessed using the sulforhodamine B method. Antioxidant activity was measured with 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assay. Results: Aqueous MP leaf exhibited cytotoxicity in all the cell lines. The highest cytotoxic activity of the test extract was observed in HeLa cells at half-maximal inhibitory concentration (IC50) = 92.8 μg/ml. Furthermore, the IC50value of the test extract when combined with Dox was significantly reduced. Specifically, in HeLa cells, the IC50was reduced by approximately 40 fold. Conclusion: This study demonstrates that aqueous MP leaf extracts could be useful as a source of antioxidants and compounds for cancer therapy. Further research is required to evaluate the chemical constituents of the leaf extracts and potential benefits for cancer therapy.


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