Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 67  |  Page : 140-147

Cytotoxicity, phagocytic activity, and leishmanicidal potential of extract standardized in geranylgeraniol obtained from the fruit of Pterodon emarginatus vogel


1 Faculty of Pharmacy, Natural Products Research Laboratory, Federal University of Goiás; Pharmacy Course, School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifical Catholic University of Goiás, Anápolis, Go, Brazil
2 Pharmacy Course, School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifical Catholic University of Goiás; Institute of Tropical Pathology and Public Health, Anápolis, Go, Brazil
3 Faculty of Pharmacy, Natural Products Research Laboratory, Federal University of Goiás, Anápolis, Go, Brazil
4 Pharmacy Course, School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifical Catholic University of Goiás; Faculty of Pharmacy Laboratory of Pharmacology and Cell Toxicology – Farmatec, Federal University of Goiás, Anápolis, Go, Brazil
5 Faculty of Pharmacy Laboratory of Pharmacology and Cell Toxicology – Farmatec, Federal University of Goiás, Anápolis, Go, Brazil
6 Pharmacy Course, School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifical Catholic University of Goiás; Pharmacy Course, University Unit of Exact and Technological Sciences, State University of Goiás, Anápolis, Go, Brazil
7 Replicon Research Center, Department of Biology, Pontifical Catholic University of Goiás, Goiânia, Brazil
8 Pharmacy Course, School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifical Catholic University of Goiás, Anápolis, Go, Brazil

Correspondence Address:
Suzana Ferreira Alves
Pharmacy Course, School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifical Catholic University of Goias
Brazil
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_231_19

Rights and Permissions

Aim: The propouse of this study was to available the cytotoxicity, phagocytic activity, and leishmanicidal potential of extract standardized from the fruits of Pterodon emarginatus Vogel. Background: P. emarginatus is a Brazilian medicinal plant with several constituents shown to have anti-inflammatory, antinociceptive, antimicrobial, and antiproliferative activities. Materials and Methods: The standardized extract in geranylgeraniol obtained from the fruit of P. emarginatus was investigated for clastogenic and cytotoxic effects, as well as phagocytic activity against Leishmania guyanensis. Results: A 24-h exposure of Allium cepa roots to standardized geranylgeraniol extract (9.77 mg/mL) promoted clastogenic effects, with a mitotic index (MI) of 10.33 ± 0.61. Extending the exposure time to 48 or 72 h significantly reduced the MI (P < 0.05). The IC50for geranylgeraniol in 3T3 and RAW 264.7 cells was found to be 15.5 mg/mL and 12.2 mg/mL, respectively. The standardized extract in geranylgeraniol exhibited high phagocytic and microbicidal activities in Leishmania, promoting a >60% increase in macrophage infection, with greater internalization and destruction of the parasite. In silico study results suggest that geranylgeraniol acts in Leishmania by interfering with the biosynthesis of sterols through steric hindrance of the active site of the enzyme CYP51. Conclusion: The standardized extract in geranylgeraniol may be useful as an antiproliferative agent, macrophage immunomodulator, and as a cytotoxic treatment against infectious agents.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed57    
    Printed0    
    Emailed0    
    PDF Downloaded15    
    Comments [Add]    

Recommend this journal