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ORIGINAL ARTICLE
Year : 2019  |  Volume : 15  |  Issue : 65  |  Page : 682-692

Bioactivity-guided isolation of cytotoxic and antioxidant phytochemicals from four Cousinia species from stenocephala bunge section


1 Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey
3 Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
4 Department of Pharmaceutical Botany, Faculty of Pharmacy, Selcuk University, Konya, Turkey
5 Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus via Mersin-10, Turkey

Correspondence Address:
Leyla Pasayeva
Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri 38030
Turkey
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_487_18

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Background: Asteraceae family contains several cytotoxic compounds bearing genus. Cousinia genus is included in the Asteraceae; it has not been studied phytochemically in detail. Objective: In this study, chemical compositions of four Cousinia species (Cousinia davisiana [CD], Cousinia foliosa, Cousinia ramosissima, and Cousinia stenocephala [CS]) were evaluated according to their cytotoxic and antioxidant effects using bioactivity-guided isolation. Materials and Methods: The cytotoxic effect was investigated with Sulphorhodamine B method against Colo205 (human colon carcinoma), A549 (human non-small cell lung cancer) cell lines, and antioxidant activity tested with 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid scavenging tests, and β-carotene/linoleic acid co-oxidation test. Purified compounds were elucidated by one-dimensional and two-dimensional nuclear magnetic resonance and mass spectroscopic techniques. The quantitative and qualitative determination of unpurified compounds within the extracts was carried out by liquid chromatography-mass spectrometry/mass spectrometry. Results: CS methanol extract, dichloromethane subextract, and FR-3 showed more cytotoxicity; isolated compound (ψ-taraxasterol) showed no cytotoxic activity. CD methanol extract and n-butanol subextract showed significant antioxidant activity. Conclusion: This is the first report that these phytochemical compounds were identified in Cousinia genus, and it is thought that these compounds could contribute to the chemotaxonomy of the genus.


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