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ORIGINAL ARTICLE
Year : 2019  |  Volume : 15  |  Issue : 65  |  Page : 621-630

Structural characterization and immune regulation of a new heteropolysaccharide from Catathelasma imperiale(Fr.) sing


1 Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, Nanchong, Sichuan Province, China
2 Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences; College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan Province, China

Correspondence Address:
Yiling Hou
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, No. 1, Shida Road, Nanchong, Sichuan Province 637009
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_673_18

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Background: Polysaccharide has played the part of great role in pharmacology and physiology. Materials and Methods: In this study, the polysaccharides (CIS-A) from Catathelasma imperiale (Fr.) Sing, were isolated and purified by hot water extraction technology and column chromatography, respectively. Chemical methods, infrared spectrum, high-performance gel-permeation chromatography, high-performance liquid chromatography, gas chromatography–mass spectrometry,1H nuclear magnetic resonance spectroscopy (NMR),13C NMR, and two-dimensional NMR were used to characterize the polysaccharides of CIS-A. The anticancer and immunomodulatory ability of the polysaccharides (CIS-A) from the fruiting body of C. imperiale (Fr.) Sing was also investigated. Results: The structural feature analysis showed the polysaccharide (CIS-A) which had a molecular weight of 50486 Da was mainly composed of α-D-glucose pyranose (α-D-Glcp) and β-L-fucose pyranose (β-L-Fucp). It had a backbone of three 1, 3-linked α-D-Glcp. There is a branch at the C2 of the polysaccharide backbone. The branches were mainly composed of two 2, 3-linked β-L-Fucp residue. Antitumor activity results showed that CIS-A could inhibit the growth of S180 tumor and promote the apoptosis of L929 cells. Immunoregulatory activity results showed that CIS-A could promote the proliferation of T-cells and promote B-cells by affecting G0/G1 phase, S phase, and G2/M phase. It also could promote the proliferation and phagocytosis of macrophages and induce cytokine release. Conclusion: Polysaccharide CIS-A can be used as a candidate drug for antitumor and immunomodulator.


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