Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2019  |  Volume : 15  |  Issue : 61  |  Page : 264-269

Standardization of enrichment protocols for some medicinally important cardenolides within in vitro grown Calotropis gigantea plantlets


1 Division of Ethnobotany, CSIR-Central Drug Research Institute, Lucknow, India
2 Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow, India
3 Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow; Faculty, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India
4 Division of Ethnobotany, CSIR-Central Drug Research Institute, Lucknow; Faculty, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India

Correspondence Address:
Dipak Kumar Mishra
Division of Ethnobotany, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow - 226 031, Uttar Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_507_18

Rights and Permissions

Background: Calotropis gigantea (L.) Dryand, belonging to the family Apocynaceae is a source of many bioactive cardenolides. However, inadequate accumulation of these cardenolides within this plant restricts their economic isolation. Due to multiple chiral centers, these metabolites could not be chemically synthesized so far. Objective: The main objective of this study was to develop protocols for enriched production of targeted bioactive cardenolides within in vitro grown plant system. Materials and Methods: In our study, we established in vitro plantlets from the seeds of naturally grown plants. Various biotic and abiotic elicitors, as well as precursors with different concentrations and incubation period, were used for the same. Results: It was observed that the in vitro grown control plantlets could biosynthesize a higher quantity of seven bioactive cardenolides than the naturally grown plants, whereas only coroglaucigenin was in less quantity. All the eight cardenolides could further successfully be enriched through our experiments. Uscharidin could be enriched significantly to a maximum level (~17-fold) followed by calotropagenin (~11-fold), uzarigenin (~8.5-folds), calotropin (4.5-fold), frugoside (~4-fold), uscharin (3.8-fold), asclepin (~2-fold), and coroglaucigenin (~1.5-fold) when they were compared to their maximum accumulation in naturally grown plants. For effective quantitative calculation of natural abundance of cardenolides within naturally grown plant, their seasonal variations were carried out using ultra high-performance liquid chromatography-mass spectrometry. Conclusion: From the above results, it can be concluded that the in vitro grown plantlets are the better choice than the naturally grown plants for enriched production of cardenolides. Elicitors were found more effective than precursors for the same. Abbreviations used: CGs: Cardiac glycosides; CH: Cholesterol; CHI: Chitosan; DDW: Double distilled water; MJ: Methyl Jasmonate; PG: Progesterone; SA: Salicylic acid; SQ: Squalene; YE: Yeast extract.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed506    
    Printed13    
    Emailed0    
    PDF Downloaded0    
    Comments [Add]    

Recommend this journal