|Year : 2019 | Volume
| Issue : 61 | Page : 183-188
Chamuangone-enriched Garcinia cowa leaf extract with rice bran oil: Extraction and cytotoxic activity against cancer cells
Pirunrat Sae-Lim1, Supreeya Yuenyongsawad1, Pharkphoom Panichayupakaranant2
1 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, Thailand
2 Department of Pharmacognosy and Pharmaceutical Botany; Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, Thailand
|Date of Submission||17-Sep-2018|
|Date of Decision||29-Oct-2018|
|Date of Web Publication||6-Mar-2019|
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Chamuangone has been isolated from Garcinia cowa leaves and exhibited various biological activities, i.e., antibacterial, anti-Leishmania major, and cytotoxic activity against cancer cells. n-Hexane has been reported to be the most suitable solvent for extraction of chamuangone. Objectives: Some vegetable oils were determined as an alternative green solvent for extraction of an anticancer compound, chamuangone from G. cowa leaf. The chamuangone-enriched extract was standardized and evaluated for cytotoxic activity against human cancer cell lines. Materials and Methods: Microwave-assisted extraction and high-performance liquid chromatography were used for extraction and standardization. The cytotoxic activity was determined using a sulforhodamine B assay. Results: The chamuangone-enriched extract was obtained using rice bran oil as the alternative green solvent and standardized to contain 1.97 mg/mL chamuangone. The extract exhibited cytotoxic activity against human lung adenocarcinoma, human breast adenocarcinoma, and human colorectal adenocarcinoma cell lines, with IC50 values of 15.3, 15.9, and 12.8 μg/mL, respectively, but was nontoxic to human gingival fibroblasts, a normal cell line, at a concentration of 50 μg/mL. Moreover, the extract contained several natural antioxidants, including α-tocopherol (76.7 mg/100 g), γ-oryzanol (cycloartenol ferulate: 67.1 μg/mL and 24-methylenecycloartanol ferulate: 85.6 μg/mL), and antioxidant capacity determined as ascorbic acid (258.7-mM ascorbic acid equivalent per gram). Conclusion: Based on these findings, the chamuangone-enriched extract may be considered as a novel functional food in cancer chemopreventive action.
Abbreviations used: AAE/g: Ascorbic acid equivalent per gram; CEO: Chamuangone-enriched Garcinia cowa leaf extract with rice bran oil; DMEM: Dulbecco's Modified Eagle Medium; FBS: Fetal bovine serum; HGF: Human gingival fibroblasts; HPLC: High-performance liquid chromatography; IC50:50% Inhibitory concentration; MAE: Microwave-assisted extraction; MHz: Megahertz; MUFAs: Monounsaturated fatty acids; NMR: Nuclear magnetic resonance; ODS: Octadecylsilane; PUFAs Polyunsaturated fatty acids; SD: Standard deviation; SFAs: Saturated fatty acids; SRB: Sulforhodamine B; TCA: Trichloroacetic acid; UFAs: Unsaturated fatty acids; UV: Ultraviolet; W: Watt.
Keywords: Anticancer, antioxidant, chamuangone, Garcinia cowa, green extraction
|How to cite this article:|
Sae-Lim P, Yuenyongsawad S, Panichayupakaranant P. Chamuangone-enriched Garcinia cowa leaf extract with rice bran oil: Extraction and cytotoxic activity against cancer cells. Phcog Mag 2019;15:183-8
|How to cite this URL:|
Sae-Lim P, Yuenyongsawad S, Panichayupakaranant P. Chamuangone-enriched Garcinia cowa leaf extract with rice bran oil: Extraction and cytotoxic activity against cancer cells. Phcog Mag [serial online] 2019 [cited 2019 Nov 15];15:183-8. Available from: http://www.phcog.com/text.asp?2019/15/61/183/253488
- Chamuangone-enriched Garcinia cowa leaf extract was prepared using rice bran oil as a green solvent
- The chamuangone-enriched extract exhibited good cytotoxic activity against cancer cells
- The chamuangone-enriched extract contained high levels of natural antioxidants
- The chamuangone-enriched extract is a novel functional food for cancer prevention.
| Introduction|| |
Cancer is a major public health problem that causes billions of deaths in most countries throughout the world., Dietary chemoprevention using fruits and vegetables as a practical, economical, and effective approach for reducing the risk of cancers has previously been described., In 2016, cancer was the leading cause of death, accounting for 8.9 million deaths globally. The most common causes of cancer death for both sexes were tracheal, bronchus, and lung cancer (1.71 million people); stomach cancer (834,171 people); colorectal cancer (829,557 people); liver cancer (828,945 people); and breast cancer (545,590 people), respectively. Although many anticancer drugs have been discovered, they have been unable to reduce the cancer mortality rate. As a result, many countries in the world are eager to find the means for decreasing the risk of cancers using dietary chemoprevention. Natural compounds from fruits and vegetables have provided many effective anticancer agents. Currently, over 50% of drugs used in clinical trials for anticancer activity have been isolated from natural products.
The cancer chemopreventive activity of vegetables, such as cabbage, broccoli, green tea, and tomatoes, along with some medicinal plants has been presented as an alternative route for cancer treatment and prevention., Vegetables are rich in vitamins, minerals, antioxidants, and phytochemicals, all of which help to protect against cancer. Presumably, the combination of these compounds in whole foods can reduce the risk of certain cancers. However, dietary antioxidant supplements for cancer prevention must be imported from abroad which can be costly. There is an enormous biodiversity in Thai vegetables that are easy to find and harvest, and it is possible that some could be great resources for detecting new anticancer agents.
Garcinia cowa Roxb. ex Choisy, called “Chamuang” in Thai, is an edible plant in the Guttiferae or Clusiaceae family. Its leaf is used in many Thai recipes, such as pork stew with chamuang leaves (in Thai “Mu-Chamuang”). Some anticancer compounds may be extracted from this recipe by lard oil obtained from streaky pork. Researchers have recently identified a new polyprenylated benzophenone named “chamuangone” that showed various biological activities, i.e., antibacterial, anti-Leishmania major, and strong cytotoxic activity against some cancer cell lines, including A549, SCB3, K562, and K562/ADM.,, In addition, a rapid method for quantitative analysis and the best extraction method of chamuangone have been described, and n-hexane has been found to be the most suitable solvent for extraction of chamuangone from G. cowa leaves using a microwave-assisted extraction (MAE). However, the use of n-hexane as a solvent for herbal industrial application was limited due to its toxicities to the respiratory and nervous systems as well as carcinogenicity.
Currently, the major concerns are reducing the risks during extraction and increasing the safety of the ingredients used. These concerns have drawn attention toward the need to use an alternative green solvent. Vegetable oils are usually used as a vehicle for nutraceutical products that are used with nonpolar active compounds. These oils have similar polarities to n-hexane but are considered to be safer and cheaper. Therefore, the present study focused on investigating the use of vegetable oils that contain different combinations of saturated, monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs), including palm oil, rice bran oil, soybean oil, and sunflower oil, as alternative green solvents for extraction of chamuangone from G. cowa leaves using MAE. In addition, the extraction processes were optimized to obtain the chamuangone-enriched G. cowa leaf extract with rice bran oil (CEO). Cytotoxicity of the standardized CEO against human cancer cell lines was also determined to obtain the preliminary anticancer activity information useful for nutraceutical applications.
| Materials and Methods|| |
G. cowa leaves were collected from Trang Province, Thailand, in November 2013. The leaves were identified by comparison with the herbarium specimen (Voucher No. SKP 083 07 03 01) which was deposited in the herbarium of the Faculty of Pharmaceutical Sciences, Prince of Songkla University, Thailand. The leaves were washed and then dried in a hot air oven at 60°C for 24 h. The dried leaves were reduced to powder using a grinder, and the powder was passed through a no. 45 sieve.
Chemicals and reagents
All analytical grade chemicals and high-performance liquid chromatography (HPLC) grade solvents were obtained from LABScan Asia (Bangkok, Thailand). Phosphate-buffered saline, penicillin-streptomycin, and Dulbecco's Modified Eagle Medium (DMEM) were purchased from Gibthai (Bangkok, Thailand). Fetal bovine serum (FBS) was acquired from Seromed (Berlin, Germany). Tris was purchased from Gibco (New York, USA), sulforhodamine B (SRB) from Sigma-Aldrich Chemie GmbH (Steinheim, Germany), and trichloroacetic acid (TCA) from CARLO ERBA Reagent S. r. l. (Cornaredo, Italy).
The human lung adenocarcinoma cell line (A549, CLS No. 300114), the human breast adenocarcinoma cell line (MCF-7, CLS No. 300273), and the human colorectal adenocarcinoma cell line (HT-29, CLS No. 300215) were obtained from Gibthai (Bangkok, Thailand), and the human gingival fibroblasts (HGF) were acquired from Faculty of Dentistry, Prince of Songkla University, Thailand.
The Agilent 1100 liquid chromatographic system equipped with a photodiode array detector and autosampler (Agilent, USA) was used for the quantitative HPLC analysis. 1H- and 13C-nuclear magnetic resonance spectra were determined using a JEOL JNM α-400 spectrometer (JEOL Ltd., Japan). A microwave (LG Electronics Inc., Thailand) was used for extraction. A microplate reader (Bio TEK Instruments Inc., Germany) and a CO2 incubator (Sheldon Manufacturing Inc., USA) were used for anticancer assay.
Quantitative high-performance liquid chromatography determination of chamuangone
HPLC analysis of chamuangone was performed using the method previously described. Separation was achieved on a Tosoh Bioscience® TSK-gel octadecylsilane-80Tm column (5-μm particle size, 4.6 mm × 150 mm). The mobile phase consisted acetonitrile and 2% v/v phosphoric acid in Milli-Q grade water (97:3, v/v), and was pumped at a flow rate of 1 mL/min. The injection volumes were 20 μL, and quantitative detection was by ultraviolet at a wavelength of 245 nm.
Purification of chamuangone
The dried powder of G. cowa leaf (1 kg) was extracted three times with n-hexane (10 L × 3) using an MAE. MAE was carried out in a laboratory scale microwave extraction apparatus, with a microwave frequency of 2450 MHz, power of 600 W, and three irradiation cycles (one cycle was 60-s power-on and 30-s power-off). The filtered solution was evaporated to dryness under reduced pressure at 40°C. The n-hexane extract was subjected to isolation and identification of chamuangone using the method previously described, with some modifications, to produce a light yellowish oil of chamuangone (81 mg). The structure of chamuangone was determined by 13C- and 1H-NMR at 100 MHz and 400 MHz, respectively, and compared with the previous report. The NMR data are as follows:13C NMR (CDCl3, 100 MHz) 17.7 (CH3, C-17), 17.8 (CH3, C-22), 17.9 (CH3, C-32), 18.1 (CH3, C-27), 21.9 (CH2, C-19), 25.7 (CH3, C-23), 25.7 (CH3, C-28), 26.0 (CH3, C-33), 28.1 (CH2, C-24), 30.4 (CH2, C-29), 38.5 (CH2, C-18), 40.9 (CH, C-7), 42.6 (CH2, C-6), 48.5 (C, C-8), 64.6 (CH, C-5), 64.8 (C, C-1), 115.6 (C, C-3), 119.6, (CH, C-30), 121.9 (CH, C-25), 123.8 (CH, C-20), 127.8 (CH, C-13), 127.8 (CH, C-15), 128.5 (CH, C-12), 128.5 (CH, C-16), 132.1 (C, C-21), 132.4 (CH, C-14), 133.4 (C, C-26), 134.8 (C, C-31), 137.1 (C, C-11), 191.4 (C, C-2), 194.6 (C, C-4), 198.0 (C, C-10), 206.5 (C, C-9). 1H NMR (CDCl3, 400 MHz) 0.95 (1H, s, H-17), 1.36 (1H, dt, J = 3.7, 12.7 Hz, H-18a), 1.44 (1H, t, J = 13 Hz, H-6ax), 1.50 (3H, overlapped, H-28), 1.55 (1H, m, H-7), 1.60 (1H, overlapped, H-24a), 1.60 (3H, overlapped, H-27), 1.63 (3H, overlapped, H-32), 1.64 (1H, overlapped, H-18b), 1.64 (3H, overlapped, H-23), 1.69 (3H, overlapped, H-22), 1.73 (3H, overlapped, H-33), 1.95 (1H, m, H-19), 2.05 (1H, dd, J = 3.2, 13 Hz, H-6eq), 2.10 (1H, overlapped, H-24b), 2.34 (1H, overlapped, H-19b), 2.35 (1H, overlapped, H-29a), 2.52 (1H, dd, J = 9.5, 13.9 Hz, H-29b), 3.4 (1H, s, H-1), 4.87 (1H, m, H-25), 5.07 (1H, m, H-20), 5.17 (1H, m, H-30), 7.35 (1H, t, J = 7.8 Hz, H-13), 7.35 (1H, t, J = 7.8 Hz, H-15), 7.45 (1H, d, J = 7.3 Hz, H-12), 7.45 (1H, d, J = 7.3 Hz, H-16), and 7.50 (1H, t, J = 7.3 Hz, H-14).
Determination of suitable vegetable oil for extraction
The powders of G. cowa leaves (2 g) were extracted with various vegetable oils (20 mL), including palm oil, rice bran oil, soybean oil, and sunflower oil, using the MAE with a microwave frequency of 2450 MHz, power of 600 W, and an irradiation period of 60 s. The leaf extracts were filtered, and the yields were recorded and then subjected to a quantitative HPLC analysis for chamuangone. The experiments were performed in triplicate.
The powders of G. cowa leaves (10 g) were extracted with rice bran oil (100 mL) using an MAE with a microwave frequency of 2450 MHz at different microwave powers of 360, 600, and 800 W, with three irradiation cycles (one cycle was 60-s power-on and 30-s power-off).
Powder to solvent ratios
The powders of G. cowa leaves in different amounts, i.e., 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 g, were separately extracted with rice bran oil (100 mL) using the MAE at a power of 800 W, with three irradiation cycles (one cycle was 60-s power-on and 30-s power-off).
The powders of G. cowa leaves (50 g) were extracted with rice bran oil (100 mL) using the MAE at a power of 800 W, with different irradiation cycles, including 2, 3, 4, 5, and 6 cycles (one cycle was 60-s power-on, and 30-s power-off).
Consecutive extraction times
The powders of G. cowa leaves (50 g) were extracted with rice bran oil (100 mL) using the MAE at a power of 800 W, with four irradiation cycles (one cycle was 60-s power-on and 30-s power-off). The extraction process was consecutively performed four times using the marc and fresh rice bran oil.
The extracts of all experiments were filtered through a filter paper. An aliquot of each sample was taken and subjected to the quantitative HPLC analysis of chamuangone. All of the experiments were performed in triplicate.
Scale-up for preparation of chamuangone-enriched Garcinia cowa leaf extract with rice bran oil
The powders of G. cowa leaves (500 g) were extracted with rice bran oil (1 L) using the MAE at a power of 900 W and four irradiation cycles (one cycle was 5-min power-on and 1-min power-off). The extracts were then filtered through a filter paper and subjected to the quantitative HPLC analysis of chamuangone as well as determinations of nutrition facts and anticancer activity.
Determination of nutrition facts
The nutrition facts of CEO were determined by a laboratory service of the Central Laboratory (Songkhla Branch) Co., Ltd (Songkhla, Thailand).
Determination of anticancer activity
The human lung adenocarcinoma (A549), human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (HT-29), and HGF cell lines were maintained in DMEM medium supplemented with 10% heat-inactivated FBS, penicillin G sodium (50 units/mL), streptomycin sulfate (50 μg/mL), and amphotericin B (0.125 μg/mL) and incubated in a humidified 5% CO2 incubator at 37°C.
Anticancer activity assay
The anticancer activity was determined using the SRB assay as described by Skehan et al. and assessed according to the protocol previously described by Keawpradub et al. Briefly, the cells were seeded into 96-well microplates at a density of 4 × 104 cells per well in 100 μL of complete medium and allowed to adhere for 24 h at 37°C in a 5% CO2 incubator. The cells were treated with 100 μL of sample solutions at various concentrations (25-μg/mL crude extract or a fivefold diluted pure compound in medium) and incubated at 37°C for 72 h. After an incubation period, the cellular proteins were fixed with 100 μL of cold 10% (w/v) TCA to each well, and the plate was incubated at 4°C for at least 1 h. The plate was then washed with water for four washing cycles, dried completely at room temperature, and stained with 50-μL 0.4% SRB solution in 1% acetic acid in each well (allowed to stain for 30 min). The dye was then dissolved in Tris base solution (pH 10.5) and shaken for 5 min. The percentage of cell growth inhibition was determined by measuring the absorbance at 492 nm (PowerWave X plate reader: BioTEK Instruments, Inc.). The activities were reported as an IC50 value. The IC50 value (effective concentration of sample required to inhibit cell growth by 50%) was calculated from dose-response curves plotting between % inhibition and concentrations. Camptothecin was used as positive control.
The results were expressed as the mean ± standard deviation. Within-group comparisons were performed by the analysis of variance using an ANOVA followed by Tukey's honestly significant difference test. Values of P < 0.05 were considered statistically significant.
| Results and Discussion|| |
Determination of an alternative solvent for extraction
Determination of vegetable oils as an alternative green solvent for extraction of chamuangone from G. cowa leaf powders using MAE found that when compared to the other solvents, palm oil produced the extract with the highest content of chamuangone as well as the highest total yield of chamuangone [Figure 1]. Although n-hexane gave the highest chamuangone content, the yield of extract was lowest due to its volatile property and led to a lower total yield of chamuangone [Table 1]. Palm oil contains a higher content of SFAs than the other vegetable oils used in this study. Thus, this may be suitable for extraction of chamuangone due to its nonpolarity that is similar to chamuangone. When compared to results from unsaturated fatty acids-rich vegetable oils, oils with higher levels of SFAs may result in a greater risk of cardiovascular diseases. Rice bran oil contains a lower level of SFAs but higher levels of MUFAs, PUFAs, and natural antioxidants than palm oil., This is more beneficial to human health than palm oil. Rice bran oil was therefore considered the most appropriate alternative green solvent for preparation of the CEO used as a novel functional food. However, the concentration of chamuangone in the extract produced by rice bran oil was rather low. Further optimization of the MAE conditions for extraction of chamuangone using rice bran oil was performed in order to increase the concentration of chamuangone in the extract.
|Figure 1: High-performance liquid chromatography chromatograms of chamuangone extracts using palm oil (a), rice bran oil (b), soybean oil (c), and sunflower oil (d) as the alternative green solvents|
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|Table 1: Chamuangone content and yields of Garcinia cowa leaf extracts obtained from various extraction oils|
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Optimization of extraction conditions
The variable factors for the MAE operating parameters included the microwave power, powder-to-solvent ratios, microwave irradiation cycles, and extraction times; each factor was determined using the single-factor experiments.
Various microwave powers, i.e., 360, 600, and 800 W were examined for extraction of chamuangone from G. cowa leaf powders using rice bran oil as the alternative solvent for extraction. An increase in microwave powers resulted in an increase in the content and total yield of chamuangone [Table 2]. This may be due to an increase in temperature during the extraction process. Heat is generated directly from the moisture that is still contained in the plant cells after exposure to the microwave irradiation. The heat generated inside plant cells can accelerate the cell leakage, and therefore, facilitate extraction efficiency. In this study, the microwave power of 800 W, which is the maximum power of this instrument, produced the highest content of chamuangone in the extract. This implies that the heat generated by this condition (145°C–150°C) can facilitate the extraction efficiency and not affect the stability of chamuangone. Therefore, the microwave power of 800 W was used for further determination of the effect of the powder-to-solvent ratio.
|Table 2: Effect of microwave powers on the chamuangone content and yields of chamuangone-enriched Garcinia cowa leaf extract with rice bran oil|
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The effect of the powder-to-solvent ratio was determined by increasing the amount of powdered leaves from 10 to 100 g/mL. An increase in the powder-to-solvent ratio resulted in increased concentration of chamuangone in the leaf extracts. However, the ratios higher than 50 g/100 mL resulted in a marked decrease in the yield of extracts, which led to a reduction of the total yield of chamuangone [Table 3]. The highest total yield of chamuangone was obtained from the ratio of 50 g/100 mL. This ratio was, therefore, selected as the most suitable ratio for preparation of chamuangone extract.
|Table 3: Effect of powder-to-solvent ratios on the chamuangone content and yields of chamuangone-enriched Garcinia cowa leaf extract with rice bran oil|
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In determining MAE irradiation cycles, 2–6 cycles found that increasing irradiation up to 4 cycles resulted in enhanced chamuangone concentration of the extracts; following this increase, the concentrations of chamuangone did not significantly increase [Table 4]. Four irradiation cycles were sufficient for the plant cell rupture and chamuangone extraction. Hence, four irradiation cycles were used for further determination of consecutive extraction times.
|Table 4: Effect of irradiation cycles on the chamuangone content and yields of chamuangone-enriched Garcinia cowa leaf extract with rice bran oil|
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The successive extractions of the sample residue, i.e., the number of consecutive extraction times, were determined. The sample residue was reextracted using fresh rice bran oil. After the first extraction, the chamuangone contents of the consecutive extracts were very low [Table 5]. It is, therefore, not worthwhile to perform the consecutive extractions using fresh solvent.
|Table 5: Effect of consecutive extraction times on the chamuangone content and yields of chamuangone-enriched Garcinia cowa leaf extract with rice bran oil|
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The optimal conditions of MAE for small-scale preparation of CEO are suggested as follows: use 50 g of dried powders of G. cowa leaves extracted with 100 mL of rice bran oil at a microwave power of 800 W, with four irradiation cycles (one cycle is 60-s power-on, and 30-s power-off), 145°C-150°C, and without consecutive extraction.
Scale-up for preparation of chamuangone-enriched Garcinia cowa leaf extract with rice bran oil
MAE conditions were determined for an extraction of 0.5-kg dried leaf powder of G. cowa with 1 L of rice bran oil using a 900-W microwave. The extraction conditions were modified based on the small-scale MAE conditions described above. An extraction efficiency of MAE is usually dependent on the temperature of extraction, which relates to the radiation power and time. The present study showed that the extraction conditions needed four irradiation cycles (one cycle is 5-min power-on and 1-min power-off), at 150°C to obtain the CEO containing chamuangone concentration up to 1.97 ± 0.01 mg/mL. This CEO was used for further studies on nutrition facts and anticancer activity.
Determination of nutrient facts
There are several nutrients derived from plants, such as vitamins, antioxidants, and phytochemicals that provided health benefits as well as cancer prevention. The CEO was enriched in MUFAs (41.89 g/100 g) and PUFAs (33.99 g/100 g), contained a low content of saturated fat (20.32 g/100 g) and no trans fat. In addition, it contained high levels of several natural antioxidants, including α-tocopherol (76.73 mg/100 g), γ-oryzanol (cycloartenol ferulate: 67.1 μg/mL and 24-methylenecycloartanol ferulate: 85.6 μg/mL), chlorophyll a (149.30 mg/100 g), chlorophyll b (2.65 mg/100 g), and antioxidant compounds determined as ascorbic acid (258.72-mM ascorbic acid equivalent per gram). Therefore, CEO does not only lower the risk of cancer but it also lowers the risk of cardiovascular diseases.
Determination of anticancer activity
The extracts giving IC50 values lower than 30 μg/mL were considered as having good cytotoxic activity. On the basis of SRB assay, chamuangone exhibited strong cytotoxic activity against HT-29 and good cytotoxic activity against MCF-7 and A549 with IC50 values of 0.02, 2.03, and 4.23 μg/mL, respectively, and no toxicity to HGF at a concentration of 25 μg/mL [Table 6]. Therefore, chamuangone was shown as selectively inhibiting the growth of the colorectal cancer cell. A similar, previously reported result showed that some polyisoprenylated benzophenones exhibited potential cytotoxic activity against several cancer cells, including breast, colon, pancreatic, and leukemia, with no cytotoxic effect against normal cells. This finding also agrees with a previous report on a selective antitumor activity of a polyprenylated compound that isolated from G. cowa. Although the CEO contained only 1.97 mg/mL chamuangone, it also showed satisfactory cytotoxicity to the cancer cells, with IC50 values of 12.82, 15.95, and 15.26 μg/mL, respectively, and had no toxicity on HGF at a concentration of 50 μg/mL. Thus, the anticancer effect of CEO might be enhanced by rice bran oil, which exhibited its own anticancer activity. Unlike chamuangone, the anticancer activity of CEO was not selective to HT-29. This may be due to a low concentration of chamuangone in CEO.
|Table 6: Cytotoxic activities of chamuangone and chamuangone enriched againt cancer and normal cell lines|
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| Conclusion|| |
Rice bran oil can be used as an alternative green solvent for extraction of chamuangone from G. cowa leaf powders using MAE. The CEO can be directly used as a functional food without the step of solvent evaporation. This extraction method is a simple and low-cost production. The standardized CEO containing 1.97 mg/mL chamuangone exhibited strong cytotoxicity against HT-29, MCF-7, and A549 cells and no toxicity to normal cells HGF. It also showed satisfactory antioxidant capacity and fatty acid balance. Therefore, the CEO may be used as a novel nutraceutical.
We wish to thank Ms. Maria Mullet for help with English language editing.
Financial support and sponsorship
This research was financially supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (grant number PHD/0129/2556).
Conflicts of interest
There are no conflicts of interest.
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[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]