Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 58  |  Page : 597-603

Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry study of the stabilities and transformations of four alisols in Alismatis Rhizoma and proprietary traditional Chinese medicine prescriptions


1 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou; Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China
2 State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China
3 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China
4 Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
5 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou; Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
6 Faculty of Chinese Medicine; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China

Correspondence Address:
Ben-Jie Zhou
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282. Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518017
China
Hua Zhou
Faculty of Chinese Medicine; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_614_17

Rights and Permissions

Background: Alismatis Rhizoma (AR) is a traditional Chinese medicine that is frequently used for the treatment of hyperlipidemia, hypertension, diabetes, and chronic kidney failure. Active alisols contribute to the beneficial effects of AR and AR products. Objective: In the present study, we investigated the stabilities and transformations of alisol B 23-acetate (B23), alisol A 24-acetate (A24), alisol A (AA), and alisol B (AB) in AR and proprietary traditional Chinese medicine prescriptions. Materials and Methods: We first established an ultra-high performance liquid chromatography/triple quadrupole mass spectrometry method for the analysis of AR products. Next, extracts and concentrates of AR and the proprietary prescriptions were prepared and heated at different temperatures. Finally, the developed method was applied to the analysis of these samples. Results: Our method is sensitive, precise, accurate, and reliable for the analysis of these four compounds. The extraction efficacies of the alisols from decoctions prepared from single herb and from mixtures of herbs showed large differences (P < 0.01). The alisols are highly sensitive to changes in temperature and pH and transform during heating. B23 can be transformed to AA and A24, and AB can be transformed to AA. Conclusion: These results indicate that the content change of aliols may affect the quality and efficacy of AR and AR products. Therefore, temperature and pH should be controlled during the manufacture of AR and AR products. Abbreviations used: AR: Alismatis Rhizoma; P1: Prescription 1; HGQZ: Hugan Qingzhi formula; P2: Prescription 2; AA: Alisol A; A24: Alisol A 24-acetate; AB: Alisol B; B23: Alisol B 23-acetate; UHPLC–QqQ–MS/MS: Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed101    
    Printed6    
    Emailed0    
    PDF Downloaded5    
    Comments [Add]    

Recommend this journal