|Year : 2018 | Volume
| Issue : 58 | Page : 471-476
Lactobacillus plantarum attenuates oxidative stress and liver injury in rats with nonalcoholic steatohepatitis
Duangporn Werawatganon1, Kanjana Somanawat1, Somying Tumwasorn2, Naruemon Klaikeaw3, Prasong Siriviriyakul1
1 Department of Physiology, Alternative and Complementary Medicine for Gastrointestinal and Liver Diseases Research Unit, Bangkok, Thailand
2 Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
3 Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
|Date of Submission||04-Jun-2018|
|Date of Acceptance||11-Jul-2018|
|Date of Web Publication||21-Nov-2018|
Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Steatohepatitis is a morphological pattern of liver injury that, in non-alcoholic patients, may represent a form of chronic liver disease currently known as non-alcoholic steatohepatitis (NASH). Probiotics, Lactobacillus sp. and Bifidobacterium sp., have been proposed to prevent and treat different inflammatory conditions of the gastrointestinal tract. Objective: To examine the effect of Lactobacillus plantarum (L. plantarum) on the liver damage of non-alcoholic steatohepatitis (NASH) rats. Materials and Methods: Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control, n = 8) was fed with phosphate-buffered saline (PBS) 1 mL/rat. Group 2 (NASH, n = 8) was fed with 100% fat diet for 6 weeks. Group 3 (NASH + L. plantarum, n = 8) was fed with 100% fat diet plus L. plantarum 1.8 × 109 colony-forming unit/mL was suspended in PBS by gavage twice a day at an interval of 4 h for 6 weeks. All rats were sacrificed to collect blood and liver samples at the end of the treatment period. Results: The levels of hepatic malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were increased while the expression of peroxisome proliferator activated receptors gamma (PPAR-γ) was decreased significantly in the NASH group as compared with the control group. Histopathology from the NASH group showed macrovesicular steatosis, hepatocyte ballooning, and lobular inflammation. The NASH + L. plantarum group had attenuated the levels of MDA and TNF-α, enhanced PPAR-γ expression, and improved the histopathology. Conclusion: L. plantarum treatment can attenuate oxidative stress, inflammation, and improvement of histopathology in rats with NASH.
Abbreviations used: NAFLD: Nonalcoholic fatty liver disease; NASH: Nonalcoholic steatohepatitis; TNF-α: Tumor necrosis factor-alpha; MDA: Malondialdehyde; PPARγ: Peroxisome proliferator-activated receptor gamma; TBARS: Thiobarbituric acid-reactive substances; ELISA: Enzyme-linked immunosorbent assay.
Keywords: Lactobacillus plantarum, liver injury, nonalcoholic steatohepatitis, oxidative stress, rats
|How to cite this article:|
Werawatganon D, Somanawat K, Tumwasorn S, Klaikeaw N, Siriviriyakul P. Lactobacillus plantarum attenuates oxidative stress and liver injury in rats with nonalcoholic steatohepatitis. Phcog Mag 2018;14:471-6
|How to cite this URL:|
Werawatganon D, Somanawat K, Tumwasorn S, Klaikeaw N, Siriviriyakul P. Lactobacillus plantarum attenuates oxidative stress and liver injury in rats with nonalcoholic steatohepatitis. Phcog Mag [serial online] 2018 [cited 2019 Jul 20];14:471-6. Available from: http://www.phcog.com/text.asp?2018/14/58/471/245851
- The effects of probiotic, L. plantarum attenuated on inflammatory and oxidative mechanisms involved in the pathogenesis of liver damage in NASH rats.
| Introduction|| |
Non-alcoholic steatohepatitis (NASH) is a liver disease characterized by macrovesicular steatosis, hepatocyte necrosis, inflammation, mallory bodies, and fibrosis.,,, NASH is closely associated with the metabolic or insulin resistance syndrome. This is a cluster of disorders, such as obesity, diabetes mellitus, dyslipidemia, arteriosclerosis, and hypertension, with insulin resistance as a common feature. In initial phases, during which fat accumulates in the liver, no clinical symptoms are evident. In advanced stages, fibrosis is detectable, which might progress into cirrhosis in some patients.
There are many models of NASH-like liver injuries in animals such as the genetic model of ob/ob mice, the methionine and choline-deficient diet model,, and a model with a high-fat liquid diet in which 71% of energy is derived from fat, 11% from carbohydrates, and 18% from protein. The fatty acid excess is converted to triglycerides and stored in the cytoplasm, predisposing the hepatocytes to oxidative stress and to activation of inflammatory pathways. In the last decade, the “2-hit” model has been proposed for the pathogenesis of NASH. Liver fat accumulation and insulin resistance characterize the first hit and are responsible for the development of steatosis. The main factors initiating the second hit are oxidative stress and subsequent lipid peroxidation, together with the production of proinflammatory cytokines, principally tumor necrosis factor-alpha (TNF-α),, and hormones derived from adipose tissue., Peroxisome proliferator-activated receptors-gamma (PPAR-γ), are members of the nuclear hormone receptor subfamily of transcription factors, from heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism, and inflammation. PPAR-γ is implicated in diseases including obesity, diabetes, atherosclerosis, and cancer. PPAR-γ activators include prostanoid, fatty acids, thiazolidinediones, and N-(2-benzoylphenyl) tyrosine analogs. PPAR-γ is a key component in adipocyte differentiation and fat-specific gene expression.,,,,,, These suggest that PPAR-γ may play an important role in the development of hepatocellular inflammation, necrosis, and fibrosis in rats with a high-fat diet.
Probiotics have been proposed to prevent and treat different inflammatory conditions of the gastrointestinal tract., These therapeutic effects might be related to a variety of direct and indirect mechanisms, including modulation of local microbiota, epithelial barrier function, and the immune system. Because the probiotic modulatory effect on the intestinal microflora could influence the gut-liver axis, these microorganisms have also been proposed as a possible adjunctive therapy in some types of chronic liver diseases., Lactobacilli are probiotics which, when administered in adequate amounts, may confer a benefit to the host., The most commonly used organisms in probiotics are Lactobacillus sp. and Bifidobacterium sp. Lactobacillus plantarum (L. plantarum) is commonly found in the human gastrointestinal tract (GI-tract). It is important in the production of a variety of fermented foods such as sauerkraut, Korean Kimchi, cheese, sausages and stockfish, and is also used as a probiotic. Importantly, L. plantarum is acid and bile tolerant, survives passage through the GI-tract, and is safe in humans and animals.
A recent meta-analysis in adult patients suggests that probiotics could be useful in non-alcoholic fatty liver disease (NAFLD) and that further research elucidating the mechanisms of such effects is needed. Preliminary data obtained in rat models of alcohol and NASH showed that the treatment with probiotics could be effective in limiting liver damage,,, but the exact mechanism of these effects is still largely undefined.
Here, we examine the effect of probiotic, L. plantarum, on inflammatory and oxidative mechanisms involved in the pathogenesis of liver damage in an experimental model of NASH rats.
| Materials and Methods|| |
This study was approved by the Ethics Committee of the Faculty of Medicine, Chulalongkorn University (IRB No. 18/57). Male Sprague-Dawley rats weighing 220–250 g from the National Laboratory Animal Center, Mahidol University, Salaya, Nakorn Pathom were used. The animals were allowed to rest for a week after arrival at the Animal Center, Department of Physiology, Faculty of Medicine, Chulalongkorn University. They were kept at a controlled temperature of 25°C ± 1°C under standard conditions (12 h dark: 12 light cycle) fed with regular dry rat chow ad libitum, and had free access to drinking water.
Bacterial strains and culture conditions
L. plantarum, isolated from Thai dyspeptic patients who visited King Chulalongkorn Memorial Hospital, was stored in de Man-Rogosa-Sharp (MRS) broth (Oxoid, Basingstoke, United Kingdom) with 20% glycerol at −80°C. This strain was recovered from frozen stock and cultivated twice on MRS agar anaerobically (10% CO2, 10% H2, and 80% N2) at 37°C in an anaerobic jar for 48 h. A single colony of L. plantarum was then inoculated into 10 mL of MRS broth and grown at 37°C under anaerobic conditions for 24 h in a 15 mL conical centrifuge tube (Corning, New York, United States).
Rats were randomly divided into three experimental groups (eight rats each) as follows.
- Group 1 (control): Rats were fed ad libitum with regular dry rat chow for 6 weeks
- Group 2 (NASH): Rats were fed ad libitum with 100% fat diet for 6 weeks to induce NASH
- Group 3 (NASH + L. plantarum): Rats were fed ad libitum with 100% fat diet for 6 weeks to induce NASH plus L. plantarum 1.8 × 109 colony-forming unit (CFUs)/mL suspended in phosphate-buffered saline 1 mL/rat by gavage twice a day at an interval of 4 h for 6 weeks.
At the end of the study, all rats were sacrificed using an intraperitoneal injection of an overdose of thiopental sodium (45 mg/kg) and the abdominal walls were opened. Blood was withdrawn by cardiac puncture for TNF-α determination using ELISA methods. The livers were excised quickly and cleaned in ice-cold nephron-sparing surgery. One lobe of the liver was frozen in liquid nitrogen and stored at −80°C for malondialdehyde (MDA) analysis. The remaining lobes of the liver were fixed in 4% paraformaldehyde in phosphate buffer solution to determine PPAR-γ expression using an immunohistochemistry method and for histological examination.
Determination of serum cytokine level
After the experiment, blood samples were taken by cardiac puncture, allowed to clot for 2 h at room temperature before centrifuging for 20 min at approximately 1000 × g. The serum was then removed and stored at −80°C for determining TNF-α level by ELISA kit (R and D systems, USA).
Hepatic malondialdehyde determination
Gastric MDA level was measured using thiobarbituric acid reactive substances assay kit (Cayman, USA). Basically, principle of the method is the reaction of one molecule of MDA and two molecules of TBA to form a red MDA-TBA complex under high temperature (90°C –100°C) and acidic conditions, which can be quantitated using a spectrophotometer at 532 nm. The assay procedures were performed as per protocol descriptions from the company. The content of MDA was expressed in terms of nmol/mg protein.
Examination of liver histopathology
The remaining liver samples were fixed in 4% paraformaldehyde in phosphate buffer solution at room temperature. They were processed by standard methods. Briefly, tissues were embedded in paraffin, sectioned at 5 μm, stained with hematoxylin and eosin (H and E), and then picked up on glass slides for light microscopy. An experienced pathologist blinded to the experiment evaluated all samples. All fields in each section were examined for grading of steatosis and necroinflammation according to the criteria described by Bacon et al.
The severity of steatosis was scored on the basis of the extent of involved parenchyma as 1 if fewer than 33% of the hepatocytes were affected, as 2 if 33%–66% of the hepatocytes were affected, as 3 if more than 66% of the hepatocytes were affected, and as 0 if no hepatocytes were affected.
Hepatic necroinflammation was graded from 0 to 3; score 1 (mild) = sparse or mild focal zone 3 hepatocyte injury/inflammation, score 2 (moderate) = noticeable zone 3 hepatocyte injury/inflammation, score 3 (severe) = zone 3 hepatocyte injury/inflammation, and score 0 = no hepatocyte injury/inflammation. Levels of hepatocytes ballooning degeneration was graded from 0 to 2; score 0 = no ballooning, score 1 = few ballooned hepatocytes, and score 2 = many ballooned hepatocytes.
Immunohistochemistry analysis of proliferator-activated receptors-gamma protein expression in liver
The liver sections were deparaffinized with xylene and ethanol for 10 min. After water washing, antigen (PPAR-γ, Santa Cruz, USA) was retrieved from the sections with citrate buffer pH 6.0 in a microwave for 13 min. Next, 3% H2O2 and 3% normal horse serum were added to the slides to block endogenous peroxidase activity for 5 min and block nonspecific binding for 20 min, respectively. The primary antibody used for PPAR-γ, a monoclonal antibody against the γ subunit of PPAR, was then applied at a dilution of 1:50 for 1 h at room temperature and incubated with the secondary antibody for 30 min. When the development of the color with DAB was detected, the slides were counterstained with hematoxylin. Under light microscopy, the positive stained cells presented dark brown in the nucleus. The results were expressed as the number of positive stained cells per high-power field.
All data were presented as mean ± standard deviation. The means were compared by one-way analysis of variance (one-way) followed by LSD Post hoc test. All statistical tests were performed using SPSS for Windows version 17.0 (SPSS Inc., Chicago, IL, United States). A P < 0.05 was considered statistically significant.
| Results|| |
Changes of serum tumor necrosis factor-alpha level
The serum TNF-α level was significantly different between NASH and control groups (3.87 ± 3.46 vs. 0.19 ± 0.30 pg/mL, P = 0.001). However, in L. plantarum 1.8 × 109 CFUs/mL treatment group, there was a significant decrease of serum TNF-α level compared with the NASH group (0.45 ± 0.07 vs. 3.87 ± 3.46 pg/mL, P = 0.003). A bar graph of serum TNF-α level of all groups is shown in [Figure 1].
|Figure 1: (a) Serum level of tumor necrosis factor alpha (b) Hepatic malondialdehyde level (c) Peroxisome proliferator activated receptors gamma positive stained cells (aP = 0.001; bP = 0.000; cP = 0.032 vs control group. dP = 0.000; eP = 0.003 vs non-alcoholic steatohepatitis (NASH).Group 1: Control; 2: NASH; 3: NASH + L. plantarum)|
Click here to view
Changes of hepatic malondialdehyde level
The level of gastric MDA increased significantly in the NASH group compared with the control group (12.41 ± 7.98 vs. 6.48 ± 4.03 nmol/mg protein, P = 0.032). After treatment for 6 weeks with 1.8 × 109 CFUs/mL of L. plantarum, there were significant decreases in elevated gastric MDA level in the NASH + L. plantarum group compared with the NASH group (1.66 ± 0.19 vs. 12.41 ± 7.98 nmol/mg protein, P = 0.000). A bar graph of hepatic MDA level of all groups is shown in [Figure 1].
There were no steatosis, hepatocyte ballooning, or lobular inflammation revealed on histology in the control group. In the NASH group, steatosis is predominantly macrovesicular, with ballooned hepatocytes and lobular inflammation noted as compared with the control group. L. plantarum 1.8 × 109 CFUs/mL treatment resulted in a significant improvement in liver histopathology of the NASH + L. plantarum group when compared with the NASH group. Liver sections from this group showed mild steatosis, hepatocyte ballooning, and lobular inflammation. Most rats in the NASH group developed steatosis and necroinflammation scores, while the NASH + L. plantarum group improved. Histological scores of steatosis and necroinflammation are summarized in [Table 1] and photomicrograph of liver histopathology is shown in [Figure 2].
|Table 1: Summary of scores of steatohepatitis and necroinflammation levels in all experimental groups and graded using Bacon et al.|
Click here to view
|Figure 2: Liver histopathology in rats with non-alcoholic steatohepatitis (H and E, ×20). (a) Control group showed normal histopathology; (b) nonalcoholic steatohepatitis group showed predominantly macrovesicular steatosis (arrowheads), hepatocytes ballooning (asterisks), and lobular inflammation (arrows); (c) nonalcoholic steatohepatitis + Lactobacillus plantarum group showed improvement of histopathology|
Click here to view
Proliferator-activated receptors-gamma protein expression
The percentage of PPAR-γ positive stained cells using the immunohistochemistry method was significantly decreased in the NASH group when compared with the control group (36.11% ± 13.57% vs. 54.34% ± 5.78%, P = 0.001). After treatment for 6 weeks with 1.8 × 109 CFUs/mL of L. plantarum, the percentage of PPAR-γ positive stained cells were significantly increased in the NASH + L. plantarum group when compared with the NASH group (75.04% ± 7.57% vs. 36.11% ± 13.57%, P = 0.000). The average percentage of PPAR-γ of all groups is shown in [Figure 1] and the immunohistochemical staining of PPAR-γ is shown in [Figure 3].
|Figure 3: Peroxisome proliferator-activated receptors-gamma positive stained cells in rats with non-alcoholic steatohepatitis (Immunohistochemistry, ×20). (a) Control group; (b) non-alcoholic steatohepatitis group showed dark brown stain in their nuclei (arrows); (c) non-alcoholic steatohepatitis + Lactobacillus plantarum group showed improvement of immunohistochemistry|
Click here to view
| Discussion|| |
Steatohepatitis is a morphological pattern of liver injury that, in non-alcoholic patients, may represent a form of chronic liver disease currently known as NASH. It is accepted that this pattern may occur in a variety of clinical settings including, but not limited to, diabetes and obesity, but in many cases, the etiology is unknown.,,,, The distinctive morphological features of steatohepatitis, regardless of the clinical background, include some “alcohol hepatitis-like” finding: steatosis; lobular inflammation, which includes polymorphonuclear leukocytes; and perisinusoidal fibrosis in zone 3 of the acinus. Other common features are hepatocellular ballooning, pooly formed Mallory's hyaline, and glycogenated nuclei.,,
To study the pathogenesis of or therapeutic options for NASH, there are many models that can be used, including a genetic model (obese rats), a model of methionine and choline deficient diet, a model of a high-fat liquid diet, and a 100% fat diet., 5, ,,,,, We showed that a 100% fat-diet fed rat was able to induce NASH; the hepatic lesions of NASH were apparent within 6 weeks. Histopathological examination showed macrovesicular steatosis, hepatocyte ballooning, and lobular inflammation.
Free fatty acid (FFA) causes oxidative stress that has the potential to induce NASH. FFA in the body is increased and this is associated with state of starvation. Stored FFA can be mobilized from adipose tissue through lipolysis. FFA metabolism increases the production of reactive oxygen species, which activated lipid peroxidation. Consequences are, the disruption of membranes and the production of reactive metabolites such as MDA. Peroxidation of phospholipids generates MDA and other MDA-like aldehydes and ketones, however, MDA is the major product that reacts with thiobarbituric acid. A high-fat diet-induced an increase in the amount of hepatic MDA.,,,,, This study found high hepatic MDA levels in 100% fat-diet fed rats in accordance with studies by others.,,,,,
Among inflammatory cytokines, TNF-α, interleukin-6, and interleukin-1 β plays a major role in the pathogenesis of the disease, contributing to systemic and hepatic insulin resistance and cellular injury, and hepatic stellate cell activation.,,, In this study, TNF-α was chosen to study inflammation in rats with 100% fat diet in the NASH model. Here, we found a significant increase in TNF-α in serum from this group.
Peroxisome PPARs-γ, members of the nuclear hormone receptor subfamily of transcription factors, form heterodimers with RXRs. These heterodimers regulate the transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism, and inflammation. PPAR-γ is implicated in diseases including obesity, diabetes, atherosclerosis, and cancer. PPAR-γ activators include prostanoid, fatty acids, thiazolidinediones, and N-(2-benzoylphenyl) tyrosine analogs. PPAR-γ is a key component in adipocyte differentiation and fat-specific gene expression.,,,,,, These suggest that PPAR-γ may play an important role in the development of hepatocellular inflammation, necrosis, and fibrosis in rats with a high-fat diet model. We found a significant decrease in PPAR-γ expression in rats with NASH. As previously reported, in a mouse model of steatohepatitis, the activation of another PPAR subtype, PPAR-α, prevented the induction of Cyclooxygenase-2 expression.
Lactobacilli are probiotics which, when administered in adequate amounts, may confer a benefit to the host. The most commonly used organisms in probiotics are Lactobacillus sp. and Bifidobacterium sp. L. plantarum is commonly found in the human GI-tract. It is important in the production of a variety of fermented foods such as sauerkraut, Korean Kimchi, cheese, sausages and stockfish, and is also used as a probiotic. Importantly, L. plantarum is acid and bile tolerant, survives passage through the GI-tract, and is safe in humans and animals.
A recent meta-analysis in adult patients suggests that probiotics could useful in NAFLD and that further research elucidating the mechanisms of such effects is needed. Preliminary data obtained in rat models of alcohol and NASH showed that treatment with probiotics could be effective in limiting liver damage,,, but the exact mechanism of these effects is still largely undefined. Interestingly, all of these studies were concordant with our results. In 100% fat-diet fed rat model, we found that L. plantarum treatments resulted in improving liver pathology, PPAR-γ expression, decreasing serum TNF-α level and hepatic MDA level. However, the mechanisms of action were unclear; these need further investigations. Our results strongly support the anti-inflammatory and anti-oxidative activity of L. plantarum probiotic, which is responsible for the preventive effect of the early onset of NASH. Another possible mechanism of the protective effect of L. plantarum may include the maintenance of gut integrity.
| Conclusion|| |
The high-fat diet induced NASH accompanied by an increased oxidative stress, inflammation, and liver histopathology. Probiotic, L. plantarum, treatment in rats with NASH could attenuate oxidative stress, inflammation, and liver histopathology.
The authors would like to thank the grant of Ratchadaphiseksomphot, Chulalongkorn University, Bangkok, Thailand.
Financial support and sponsorship
The Grant of Ratchadaphiseksomphot, Chulalongkorn University, Bangkok, Thailand.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Chalasani N, Deeg MA, Crabb DW. Systemic levels of lipid peroxidation and its metabolic and dietary correlates in patients with nonalcoholic steatohepatitis. Am J Gastroenterol 2004;99:1497-502.
Te Sligte K, Bourass I, Sels JP, Driessen A, Stockbrugger RW, Koek GH, et al.
Non-alcoholic steatohepatitis: Review of a growing medical problem. Eur J Intern Med 2004;15:10-21.
DeFronzo RA. Insulin resistance: A multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidaemia and atherosclerosis. Neth J Med 1997;50:191-7.
Medina J, Fernández-Salazar LI, García-Buey L, Moreno-Otero R. Approach to the pathogenesis and treatment of nonalcoholic steatohepatitis. Diabetes Care 2004;27:2057-66.
Campfield LA, Smith FJ, Burn P. The OB protein (leptin) pathway – A link between adipose tissue mass and central neural networks. Horm Metab Res 1996;28:619-32.
Weltman MD, Farrell GC, Liddle C. Increased hepatocyte CYP2E1 expression in a rat nutritional model of hepatic steatosis with inflammation. Gastroenterology 1996;111:1645-53.
Koteish A, Diehl AM. Animal models of steatosis. Semin Liver Dis 2001;21:89-104.
Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C, et al.
Model of nonalcoholic steatohepatitis. Am J Clin Nutr 2004;79:502-9.
Boden G, She P, Mozzoli M, Cheung P, Gumireddy K, Reddy P, et al.
Free fatty acids produce insulin resistance and activate the proinflammatory nuclear factor-kappaB pathway in rat liver. Diabetes 2005;54:3458-65.
Day CP. Pathogenesis of steatohepatitis. Best Pract Res Clin Gastroenterol 2002;16:663-78.
Farrell GC, Larter CZ. Nonalcoholic fatty liver disease: From steatosis to cirrhosis. Hepatology 2006;43:S99-112.
McCullough AJ. Pathophysiology of nonalcoholic steatohepatitis. J Clin Gastroenterol 2006;40 Suppl 1:S17-29.
Duvnjak M, Lerotić I, Barsić N, Tomasić V, Virović Jukić L, Velagić V, et al.
Pathogenesis and management issues for non-alcoholic fatty liver disease. World J Gastroenterol 2007;13:4539-50.
Kojima H, Sakurai S, Uemura M, Fukui H, Morimoto H, Tamagawa Y, et al.
Mitochondrial abnormality and oxidative stress in nonalcoholic steatohepatitis. Alcohol Clin Exp Res 2007;31:S61-6.
Huang JT, Welch JS, Ricote M, Binder CJ, Willson TM, Kelly C, et al.
Interleukin-4-dependent production of PPAR-gamma ligands in macrophages by 12/15-lipoxygenase. Nature 1999;400:378-82.
Yuan H, Upadhyay G, Yin Y, Kopelovich L, Glazer RI. Stem cell antigen-1 deficiency enhances the chemopreventive effect of peroxisome proliferator-activated receptorγ activation. Cancer Prev Res (Phila) 2012;5:51-60.
Cai Y, Fan C, Yan J, Tian N, Ma X. Effects of rutin on the expression of PPARγ in skeletal muscles of db/db mice. Planta Med 2012;78:861-5.
Siersbæk MS, Loft A, Aagaard MM, Nielsen R, Schmidt SF, Petrovic N, et al.
Genome-wide profiling of peroxisome proliferator-activated receptor γ in primary epididymal, inguinal, and brown adipocytes reveals depot-selective binding correlated with gene expression. Mol Cell Biol 2012;32:3452-63.
Alimirah F, Peng X, Yuan L, Mehta RR, von Knethen A, Choubey D, et al.
Crosstalk between the peroxisome proliferator-activated receptor γ (PPARγ) and the Vitamin D receptor (VDR) in human breast cancer cells: PPARγ binds to VDR and inhibits 1α,25-dihydroxyvitamin D3 mediated transactivation. Exp Cell Res 2012;318:2490-7.
Soares VM, Garcia-Souza EP, Lacerda-Miranda G, Moura AS. Early life overfeeding decreases acylated ghrelin circulating levels and upregulates GHSR1a signaling pathway in white adipose tissue of obese young mice. Regul Pept 2012;174:6-11.
Hervouet E, Nadaradjane A, Gueguen M, Vallette FM, Cartron PF. Kinetics of DNA methylation inheritance by the dnmt1-including complexes during the cell cycle. Cell Div 2012;7:5.
Mach T. Clinical usefulness of probiotics in inflammatory bowel diseases. J Physiol Pharmacol 2006;57 Suppl 9:23-33.
Ulisse S, Gionchetti P, D'Alò S, Russo FP, Pesce I, Ricci G, et al.
Expression of cytokines, inducible nitric oxide synthase, and matrix metalloproteinases in pouchitis: Effects of probiotic treatment. Am J Gastroenterol 2001;96:2691-9.
O'Hara AM, Shanahan F. Mechanisms of action of probiotics in intestinal diseases. ScientificWorldJournal 2007;7:31-46.
Loguercio C, De Simone T, Federico A, Terracciano F, Tuccillo C, Di Chicco M, et al.
Gut-liver axis: A new point of attack to treat chronic liver damage? Am J Gastroenterol 2002;97:2144-6.
Loguercio C, Federico A, Tuccillo C, Terracciano F, D'Auria MV, De Simone C, et al.
Beneficial effects of a probiotic VSL#3 on parameters of liver dysfunction in chronic liver diseases. J Clin Gastroenterol 2005;39:540-3.
Lirussi F, Azzalini L, Orando S, Orlando R, Angelico F. Antioxidant supplements for non-alcoholic fatty liver disease and/or steatohepatitis. Cochrane Database Syst Rev 2007:CD004996.
Fuller R. Probiotics in man and animals. J Appl Bacteriol 1989;66:365-78.
Saxelin M, Tynkkynen S, Mattila-Sandholm T, de Vos WM. Probiotic and other functional microbes: From markets to mechanisms. Curr Opin Biotechnol 2005;16:204-11.
Nanji AA, Khettry U, Sadrzadeh SM. Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver (disease). Proc Soc Exp Biol Med 1994;205:243-7.
Solga SF, Diehl AM. Non-alcoholic fatty liver disease: Lumen-liver interactions and possible role for probiotics. J Hepatol 2003;38:681-7.
Li Z, Yang S, Lin H, Huang J, Watkins PA, Moser AB, et al.
Probiotics and antibodies to TNF inhibit inflammatory activity and improve nonalcoholic fatty liver disease. Hepatology 2003;37:343-50.
Brunt EM, Janney CG, Di Bisceglie AM, Neuschwander-Tetri BA, Bacon BR. Nonalcoholic steatohepatitis: A proposal for grading and staging the histological lesions. Am J Gastroenterol 1999;94:2467-74.
Ludwig J, Viggiano TR, McGill DB, Oh BJ. Nonalcoholic steatohepatitis: Mayo clinic experiences with a hitherto unnamed disease. Mayo Clin Proc 1980;55:434-8.
Ludwig J, McGill DB, Lindor KD. Review: Nonalcoholic steatohepatitis. J Gastroenterol Hepatol 1997;12:398-403.
Bacon BR, Farahvash MJ, Janney CG, Neuschwander-Tetri BA. Nonalcoholic steatohepatitis: An expanded clinical entity. Gastroenterology 1994;107:1103-9.
Powell EE, Cooksley WG, Hanson R, Searle J, Halliday JW, Powell LW, et al.
The natural history of nonalcoholic steatohepatitis: A follow-up study of forty-two patients for up to 21 years. Hepatology 1990;11:74-80.
Abdelmalek M, Ludwig J, Lindor KD. Two cases from the spectrum of nonalcoholic steatohepatitis. J Clin Gastroenterol 1995;20:127-30.
Neuschwander-Tetri BA, Bacon BR. Nonalcoholic steatohepatitis. Med Clin North Am 1996;80:1147-66.
Diehl AM, Goodman Z, Ishak KG. Alcohollike liver disease in nonalcoholics. A clinical and histologic comparison with alcohol-induced liver injury. Gastroenterology 1988;95:1056-62.
Lee RG. Nonalcoholic steatohepatitis: A study of 49 patients. Hum Pathol 1989;20:594-8.
Itoh S, Yougel T, Kawagoe K. Comparison between nonalcoholic steatohepatitis and alcoholic hepatitis. Am J Gastroenterol 1987;82:650-4.
Ludwig J, Dickson ER, McDonald GS. Staging of chronic nonsuppurative destructive cholangitis (syndrome of primary biliary cirrhosis). Virchows Arch A Pathol Anat Histol 1978;379:103-12.
Thong-Ngam D, Samuhasaneeto S, Kulaputana O, Klaikeaw N. N-acetylcysteine attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis. World J Gastroenterol 2007;13:5127-32.
Esposito E, Iacono A, Bianco G, Autore G, Cuzzocrea S, Vajro P, et al.
Probiotics reduce the inflammatory response induced by a high-fat diet in the liver of young rats. J Nutr 2009;139:905-11.
Benzie IF. Lipid peroxidation: A review of causes, consequences, measurement and dietary influences. Int J Food Sci Nutr 1996;47:233-61.
Fan JG, Zhong L, Xu ZJ, Tia LY, Ding XD, Li MS, et al.
Effects of low-calorie diet on steatohepatitis in rats with obesity and hyperlipidemia. World J Gastroenterol 2003;9:2045-9.
Leclercq IA, Farrell GC, Field J, Bell DR, Gonzalez FJ, Robertson GR, et al.
CYP2E1 and CYP4A as microsomal catalysts of lipid peroxides in murine nonalcoholic steatohepatitis. J Clin Invest 2000;105:1067-75.
Kirsch R, Clarkson V, Shephard EG, Marais DA, Jaffer MA, Woodburne VE, et al.
Rodent nutritional model of non-alcoholic steatohepatitis: Species, strain and sex difference studies. J Gastroenterol Hepatol 2003;18:1272-82.
George J, Pera N, Phung N, Leclercq I, Yun Hou J, Farrell G, et al.
Lipid peroxidation, stellate cell activation and hepatic fibrogenesis in a rat model of chronic steatohepatitis. J Hepatol 2003;39:756-64.
Cortez-Pinto H, de Moura MC, Day CP. Non-alcoholic steatohepatitis: From cell biology to clinical practice. J Hepatol 2006;44:197-208.
Arkan MC, Hevener AL, Greten FR, Maeda S, Li ZW, Long JM, et al.
IKK-beta links inflammation to obesity-induced insulin resistance. Nat Med 2005;11:191-8.
Ding WX, Yin XM. Dissection of the multiple mechanisms of TNF-alpha-induced apoptosis in liver injury. J Cell Mol Med 2004;8:445-54.
Yu J, Ip E, Dela Peña A, Hou JY, Sesha J, Pera N, et al.
COX-2 induction in mice with experimental nutritional steatohepatitis: Role as pro-inflammatory mediator. Hepatology 2006;43:826-36.
[Figure 1], [Figure 2], [Figure 3]