Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 57  |  Page : 417-429

Stimulation of murine immune response by Clerodendrum infortunatum


1 Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India
2 Department of Botany, Molecular Genetics Laboratory, University of North Bengal, Siliguri, West Bengal, India
3 Department of Human Sciences, Ohio State University, Columbus, OH, USA
4 Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India; Department of Human Sciences, Ohio State University, Columbus, OH, USA
5 Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal; Department of Zoology, Bodoland University, Kokrajhar, Assam, India

Correspondence Address:
Tapas Kumar Chaudhuri
Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri - 734 013, West Bengal

Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_549_17

Rights and Permissions

Background: Clerodendrum infortunatum is a medicinal plant found especially in the Sub-Himalayan regions of West Bengal. Various tribal communities of this region have been using these plants as a source of natural medicine. Objective: Medicinal values of C. infortunatum are well established, but the immunomodulatory properties have never been studied. Therefore, we wanted to investigate various immunomodulatory activities of this plant. Materials and Methods: Several parameters such as plaque-forming cell assay, hemagglutination titer, phagocytic activity of macrophages, and inhibition of lipopolysaccharide (LPS) were performed. Moreover, effects of C. infortunatum on the weight of various organs and total serum protein, albumin, and globulin levels were also determined. The phytochemical fingerprints of C. infortunatum extracts were obtained from Fourier transform infrared and gas chromatography-mass spectrometry analysis. Results: The macrophage stimulation and plaque-forming cell numbers (using sheep red blood cell as antigen) were significantly proliferated and found optimum at 100 and 250 mg/kg doses of leaf and root extracts simultaneously. The extent of stimulation in mural humoral immunity was in the order of leaf > root > stem. The anti-inflammatory activity of C. infortunatum was confirmed by the inhibition of LPS-induced nitric oxide synthesis by macrophages. Conclusion: It can be concluded that 70% hydromethanolic extract of C. infortunatum has profound immunomodulation potentials in the murine model, stimulating both humoral and innate form of the immune system. Abbreviations used: PFC: Plaque-forming cell assay; HA titer: Hemagglutination titer; LPS: Lipopolysaccharide; FTIR: Fourier transform infrared; GC-MS: Gas chromatography-mass spectrometry; SDS: Sodium dodecyl sulfate; sRBC: Sheep red blood cell; PBS: Phosphate buffer saline; PC: Phagocytic capacity; PI: Phagocytic index; NaCl: Sodium chloride; CaCl2: Calcium chloride; NBT: Nitro blue tetrazolium; DMSO: Dimethyl sulfoxide; KOH: Potassium hydroxide; H2SO4: Sulfuric acid; HOCl: Hypochlorous acid; H2O2: Hydrogen peroxide; EDTA: Ethylenediaminetetraacetic acid.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed282    
    Printed8    
    Emailed0    
    PDF Downloaded6    
    Comments [Add]    

Recommend this journal