Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 57  |  Page : 384-392

Identification of phytoconstituents of Memecylon sisparense gamble leaf and evaluation against cisplatin-induced oxidative renal damage in mice


1 Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER-Hyderabad), Hyderabad; Department of Biotechnology, VFSTR (Deemed to be University), Guntur, Andhra-Pradesh, India
2 Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER-Hyderabad), Hyderabad, Telangana, India
3 Metabolomics Facility, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, India
4 Department of Microbiology, Sri Shivani College of Pharmacy, Warangal, Telangana, India
5 Department of Biotechnology, VFSTR (Deemed to be University), Guntur, Andhra-Pradesh, India

Correspondence Address:
Asha Syed
Department of Biotechnology, VFSTR (Deemed to be University), Guntur, Andhra Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_116_18

Rights and Permissions

Background: Memecylon sisparense Gamble (MSG) belongs to Melastomataceae family, having a wide range of pharmacological activities such as antioxidant, hepatoprotective, and anti-inflammatory. Objective: The present study aimed for the first time toward the identification of biologically active compounds in MSG leaf ethyl acetate extract (MSGLEAE) by gas chromatography–mass spectrometry (GC-MS) analysis and compared with docking studies along with its nephroprotective activity against cisplatin (CP)-induced nephrotoxicity in mice. Materials and Methods: MSGLEAE was subjected to GC-MS analysis and molecular docking studies. Swiss albino male mice were treated with MSGLEAE (250, 500 mg/kg, PO) against CP 12 mg/kg IP evaluated for nephroprotective activity. The changes in renal tissue were assessed from serum biochemical renal toxicity, antioxidant stress markers along with histopathological studies. Results: Out of 41 compounds identified, 20 were found having biological activities such as nephroprotective, hepatoprotective, anticancer, antioxidant, and antimicrobial and inhibition of uric acid production. The nephroprotective active compounds (N,N,O-triacetylhydroxylamine, 2(4H)-benzofuranone, 5,6,7,7a-tetrahydro-4,4,7a-trimethyl-, N-{(4-hydroxy-3-methoxyphenyl) methyl}-8-methyl-6-nonenamide) had shown binding energy of −5.27, −5.98, −5.27 (ΔG(Kcal/mol)), respectively, in docking studies. MSGLEAE showed a significant protective effect against CP-induced nephrotoxicity because of the identified compounds by reducing the oxidative stress in renal tissue evident by histopathological studies. Conclusion: This is the first ever report in terms of identification of bioactive constituents in MSGLEAE. Pretreatment has a significant therapeutic benefit during CP therapy by inhibiting oxidative stress, enhancing nephroprotective activity. Abbreviations used: MSGLEAE: Memecylon sisparense Gamble leaf ethyl acetate extract; CP: Cisplatin; BUN: Blood urea nitrogen; SOD: Superoxide dismutase; CAT: Catalase; GSH: Glutathione; NO: Nitric oxide; MDA: Malondialdehyde; IAEC: Institutional Animal Ethics Committee; TCA: Trichloroacetic acid; DTNB: 5,51-dithiobis-2-nitrobenzoic acid; FC: Folin–Ciocalteu reagent; SNP: Sodium nitroprusside; H and E: Hematoxylin and eosin.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed834    
    Printed29    
    Emailed0    
    PDF Downloaded5    
    Comments [Add]    

Recommend this journal