Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 57  |  Page : 284-293

Evaluation of antiangiogenic potential of Psidium guajava leaves using In-Ovo chick chorioallantoic membrane assay


1 Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, University of Delhi, New Delhi, India
2 Department of Pharmacology, Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India

Correspondence Address:
Rajani Mathur
Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, University of Delhi, Pushp Vihar, Sector-3, MB Road, New Delhi - 110 017
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_133_18

Rights and Permissions

Background: Angiogenesis is the process of formation of new blood vessels from the existing one. Pathological angiogenesis is widely implicated in many diseases, including cancer, diabetic neuropathy, retinopathy, obesity, and arthritis. Objective: The present study was aimed to evaluate the in vitro antioxidant and in ovo antiangiogenic activity of aqueous extract of Psidium guajava leaves (AEPG). Materials and Methods: Psidium guajava commonly known as guava reported to contain polyphenols and flavonoids such as gallic acid, epigallocatechin, catechin, rutin, and quercetin in glycosidic forms in its leaves. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), nitric oxide, hydrogen peroxide, hydroxyl, and superoxide radical scavenging assays (RSAs) and antiangiogenic activity was evaluated using vascular endothelial growth factor (VEGF)-induced chick chorioallantoic membrane (CAM).The correlation between the antioxidant and antiangiogenic activity was correlated with total phenolic content (TPC) of AEPG. Results: The TPC of AEPG was found to be 493.8 ± 8.9 mg of GAE/g. The total flavonoid content of AEPG was found to be 254.9 ± 13.7 mg of CE/g. In vitro antioxidant activity of AEPG showed IC50 values of 19.4 ± 1.9, 25.5 ± 0.2, 4.9 ± 0.5, 29.9 ± 2.06, 39.5 ± 2.07, and 29.9 ± 0.9 μg/ml, respectively, for DPPH, ABTS, nitric oxide, hydrogen peroxide, hydroxyl, and superoxide RSAs. Significant reduction in angiogenesis in the AEPG treated groups when compared to untreated VEGF groups and the Pearson's correlation coefficient between TPC of AEPG and total length, area, branches of blood vessels and CAM thickness were −0.9261, −0.9807, −0.9637, and −0.9597, respectively. Conclusion: The results revealed potent antiangiogenic activity of AEPG leaves and exhibit significant correlation between the antioxidant and antiangiogenic activity of AEPG and its TPC. Abbreviations used: EGF: Epidermal growth factor; FGF: Fibroblast growth factor; G-CSF: Granulocyte colony stimulating factor; IL: Interleukin; INF: Interferon; MMP: Matrix metalloproteinases; NOS: Nitric oxide synthase; PAF: Platelet-activating factor; PAI: Plasminogen activator inhibitor; PDGF: Platelet-derived growth factor; PG-E: Prostaglandin E; RSA: Radical scavenging assay; TFC: Total flavonoid content; TPC: Total Phenolic content; TIMP: Tissue inhibitors of metalloproteinases; TNF-α: Tumor necrosis factor alpha; VEGF: Vascular endothelial growth factor.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed184    
    Printed5    
    Emailed0    
    PDF Downloaded5    
    Comments [Add]    

Recommend this journal