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ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 55  |  Page : 36-39

Insulinotropic and cytoprotective effect of L-theanine: An in vitro dose dependent study


1 Department of Biochemistry, KPC Medical College, Kolkata, India
2 Department of Biotechnology, Techno India University, Kolkata, India
3 Department of Medicine, KPC Medical College, Kolkata, India
4 Department of Endocrinology, IPGME and R, Kolkata, West Bengal, India

Correspondence Address:
Sandip K Bandyopadhyay
Department of Biochemistry, KPC Medical College, Kolkata - 700 032, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_595_17

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Background: L-theanine is a natural bioactive amino acid present in tea (Camellia sinensis [L.] O. Kuntze) and consumed worldwide through tea decoction. Objective: The present work aims at evaluation of the insulin secretion and cytoprotective efficacy of L-theanine, a bioactive amino acid present in tea, on pancreatic β-cell line, RIN-m5F. Methods: RIN m5F cell were treated with L-theanine alone or prior to hydrogen peroxide (200 um) treatment and subsequently cell viability, cellular morphology, insulin secretion and insulin gene expression were analyzed. Results: Studies have shown that L-theanine dose dependently (0–300 μm) increases the β-cell mass as well as increases insulin production by RIN-m5F cell. It was also observed that pretreatment of the cell with L-theanine partially protected the oxidative stress of β-cells that were evident from cell viability, cellular morphology, and restoration of insulin-secreting ability. Conclusion: Results suggest that L theanine is an insulinotropic agent as well as effective in giving partial protection to pancreatic β-cells in oxidatively stressed condition. L-theanine can be used in the prevention or treatment of diabetes. Abbreviations used: EGCG: Epigallocatechin gallate; FBS: Fetal Bovine Serum; DMEM Dulbecco's Modified Eagle Medium


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