|Year : 2018 | Volume
| Issue : 55 | Page : 167-174
6-Gingerol prevents free transition metal Ion [Fe (Ii)] Induced free radicals mediated alterations by In vitro and Ndv growth in chicken eggs by In ovo
Ganjikunta Venkata Subbaiah1, Kesireddy Sathyavelu Reddy1, Yagani JayavardhanaRao2, Bhasha Shanmugam1, Sahukari Ravi1, Kondeti. Ramudu Shanmugam1, Golla Narasimha2
1 Department of Zoology, Division of Molecular Biology and Ethnopharmacology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India
2 Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India
|Date of Submission||31-Aug-2017|
|Date of Acceptance||19-Oct-2017|
|Date of Web Publication||28-Jun-2018|
Ganjikunta Venkata Subbaiah
Department of Zoology, Division of Molecular Biology and Ethnopharmacology, Sri Venkateswara University, Tirupati - 517 502, Andhra Pradesh
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: The study of free radicals and their reactions is implicated in the pathogenesis of various human diseases. Therefore, antioxidants treatment plays a pivotal role in preventing the free radical-induced diseases. Objective: The objectives of this study were to examine the protective role of 6-gingerol from free transition metal ion-induced alterations by in vitro and Newcastle disease virus (NDV) in chicken eggs by in ovo. Materials and Methods: In this study, lipid peroxidation, DNA sugar oxidation, chelation of ferrous ions, DNA fragmentation, Cytochrome c-oxidation, ferric ion reducing activities and DPPH, superoxide and hydroxyl radicals scavenging activity by ESR studies were examined with 6-gingerol by in vitro. Furthermore, antiviral activity was screened with 6-gingerol by in ovo. Results: In vitro results showed that 254 μM/ml of 6-gingerol firmly inhibited erythrocyte membrane lipid peroxidation by 85.28% and DNA sugar oxidation by 91.26% when compared with butylated hydroxyanisole 74.42% (554 μM/ml) and 78.30% (824 μM/ml), respectively. Whereas, chelating activity of 6-gingerol was 80.2% which nearly similar to the EDTA activity (50 μg/ml) shown 83.93%. In addition, 6-gingerol (254μM/ml) scavenged the superoxide, hydroxyl, DPPH radicals by 84.96%, 90.45%, and 94.63% respectively and the same was strongly supported with ESR studies. Further, 254 μM/ml of 6-gingerol inhibited the DNA fragmentation, Cytochrome c-oxidation and reducing the ferric ion by in vitro and antiviral activity shown on NDV at 100 μg/ml. Conclusion: This study could enlighten that the 6-gingerol has a good metal sequester property and it could prevent the free radicals by in vitro and NDV growth in chicken eggs by in ovo.
Abbreviations used: NDV: Newcastle Disease Virus; ESR: Electron Spin resonance; SOD: Super oxide Dismutase; HA: Hemagglutination.
Keywords: 6-Gingerol, antioxidants, cytochrome c, DNA fragmentation, lipid peroxidation
|How to cite this article:|
Subbaiah GV, Reddy KS, JayavardhanaRao Y, Shanmugam B, Ravi S, Shanmugam KR, Narasimha G. 6-Gingerol prevents free transition metal Ion [Fe (Ii)] Induced free radicals mediated alterations by In vitro and Ndv growth in chicken eggs by In ovo. Phcog Mag 2018;14, Suppl S1:167-74
|How to cite this URL:|
Subbaiah GV, Reddy KS, JayavardhanaRao Y, Shanmugam B, Ravi S, Shanmugam KR, Narasimha G. 6-Gingerol prevents free transition metal Ion [Fe (Ii)] Induced free radicals mediated alterations by In vitro and Ndv growth in chicken eggs by In ovo. Phcog Mag [serial online] 2018 [cited 2020 May 30];14, Suppl S1:167-74. Available from: http://www.phcog.com/text.asp?2018/14/55/167/235271
- 6-gingerol prevents free transition metal ion induced alterations
- 6-gingerol protects the DNA from metal ions induced free radicals
- 6-gingerol inhibits the NDV growth in chicken eggs.
| Introduction|| |
The study of free radicals and their reactions has been led to increase the interest in the pathogenesis of human diseases. However, free transition metal ions induced free radicals interact the biomolecules and caused the oxidative damage. Thus, they play a major role in the contribution of oxidative stress-related diseases. However, all aerobic animals have antioxidant defense system to counteract the harmful effects induced by free radicals. Sometimes, these protective mechanisms are disrupted by various pathological conditions due to an increase of oxidative stress.
Newcastle disease virus (NDV) causes Newcastle disease in all domestic and wild avian species. Its effect the egg production, hemorrhagic intestinal lesions, nervous disorders and respiratory distress. Consequently, its effect on economic status of food production sectors in all around the world because of high mortality and morbidity was associated with disease. Moreover, viral infections are accompanied with profound changes in cell/tissue metabolism, which lead to generate the reactive oxygen species. In this scenario, antioxidant supplementations were needed to overcome the oxidative stress. Although a number of synthetic antioxidants accomplish to be promising, their toxicity and side effects were restricted on their extensive prescription.
The phenolic compounds of plants have gained much attention due to their free radical scavenging and antioxidant activities with potential beneficial implications in human and animals health. When they consumed with food, antioxidants minimize rancidity, retard the formation of toxic oxidation products, maintain nutritional quality, and increase shelf life. Many medicinal plants have shown good antimicrobial activity. Several epidemiological studies have also been reported that the consumption of natural antioxidants reduced the risk of a number of chronic diseases.,
Ginger (Zingiber officinale) belongs to Zingiberaceae family. Since over 2000 years, ginger rhizome has been used worldwide as a spice and flavoring agent. Ginger has a long history of medicinal use for various diseases including dementia, fever, infectious diseases, arthritis, and diabetes. Ginger rhizome contains biological active constituents including gingerol. Gingerols are found in greater abundance in fresh ginger. Gingerol exhibit anti-inflammatory, antihyperglycemic, and anticancer activity. To the best of our knowledge, there is no report related to ginger volatile compound 6-gingerol effect on free transition metal ion-induced free radical-mediated alterations and antiviral activity against NDV by in vitro and in ovo conditions. Therefore, we investigated the transition metal ion-induced free radical-mediated alterations and antiviral activity against NDV by 6-gingerol.
| Materials and Methods|| |
6-Gingerol was purchased from TCI chemicals, China. 5,5'-dimethyl-l-pyrroline-N-oxide (DMPO), xanthine oxidase, cytochrome c, superoxide dismutase (SOD), and calf thymus DNA were purchased from Sigma-Aldrich, USA; remaining all chemicals were analytical grade and purchased from Sigma and HiMedia.
In vitro studies
Erythrocyte membrane lipid peroxidation
Lipid peroxidation was induced in erythrocyte ghost by adopting the method of Dodge et al. Lipid peroxidation was measured by the assessment of thiobarbituric acid reactive substances using Dahle et al.'s  method. Briefly, 6-gingerol was added at various concentrations ranging from 10 to 100 μg/ml to the reaction mixture. Butylated hydroxyanisole (BHA) was used as a positive control. The absorbance was measured calorimetrically at 535 nm. The percent inhibition of lipid peroxidation was calculated by comparing the absorbance of the test samples with negative control.
Fenton reactant-induced DNA sugar damage
Oxidative DNA sugar damage was measured by the following Sultan et al.'s  method. Briefly, 6-gingerol at various concentrations ranging from 10 to 100 μg/ml was added to the reaction mixture and absorbance was read at 535 nm using a Shimadzu ultraviolet (UV)-1800 Spectrophotometer (Tokyo, Japan). BHA was used as a positive control. The percent inhibition of DNA oxidation was measured by comparing the absorbance of the test sample with a negative control.
Hydroxyl radical-mediated DNA damage
DNA damage was measured on agarose gel by deoxyribose assay. Briefly, 100 μg of calf thymus DNA was added to the 100 μl of reaction mixture and 10 μl of loading buffer was run on 1% agarose with ethidium bromide. The electrophoresis was conducted and DNA was visualized under a UV transilluminator (Bio-Rad, Sydney, Australia). BHA (50 and 100 μg/ml) was used as positive control.
Cytochrome c reduction
The cytochrome c-reducing capacity of 6-gingerol was measured using the method of Suter and Richter. Briefly, 6-gingerol was added (10–100 μg) to the oxidative cytochrome c and absorbance was assessed at 550 nm using a Shimadzu UV-1800 Spectrophotometer. BHA was used as positive control.
Test for ferric ion-reducing capacity (Fe 3+ to Fe 2+)
The ferric ion-reducing capacity of 6-gingerol was assessed using the method followed by Wang et al. Briefly, 6-gingerol was added at various concentrations ranging from 10 to 100 μg to the reaction mixture. The absorbance was recorded at 700 nm using Shimadzu UV-1800 spectrophotometer. Absorbance increases with an increase in ferric ion-reducing capacity.
Test for ferrous ion-chelating activity
Ferrous ion-chelating activity of 6-gingerol was assessed by the method proposed by Suter and Richter. 6-gingerol at various concentrations ranging from 10 to 100 μg was added to the reaction mixture. The absorbance was measured at 700 nm using Shimadzu UV-1800 Spectrophotometer. Ethylenediaminetetraacetic acid (EDTA) (10–100 μg) was used as positive control. The percent of ferrous ion-chelating activity was measured by comparing the absorbance of the test samples with that of the negative control.
Superoxide radical (O −2)-scavenging activity was measured using the method followed by Lee et al. Briefly, 6-gingerol was added at various concentrations ranging from 10 to 100 μg to the reaction mixture. The absorbance was measured calorimetrically at 560 nm. SOD (10–100 μg/3 ml) was served as positive control. The percent superoxide-scavenging activity was determined accordingly by comparing the absorbance of test samples with the negative control.
Hydroxyl radical-scavenging activity
Hydroxyl radical-scavenging activity was measured by deoxyribose assay. Briefly, 6-gingerol was added at various concentrations ranging from 10 to 100 μg to the reaction mixture and absorbance was measured at 532 nm. BHA was used as positive control. The percent inhibition of hydroxyl radical-scavenging activity of 6-gingerol was evaluated by comparing with negative control.
1,1-Diphenyl-2-picrylhydrazyl radical-scavenging activity
1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity was evaluated by the method of Shimada et al. Briefly, 6-gingerol was added to the reaction mixture at various concentrations ranging from 10 to 100 μg/ml. The absorbance was read at 517 nm in a Shimadzu UV-1800 Spectrophotometer. BHA at 400 μM was used as positive control. The percent DPPH radical-scavenging activity of 6-gingerol was calculated by decrease in absorbance at 517 nm in comparison with the negative control.
Free radical-scavenging activity of 6-gingerol by electron spin resonance
Free radical-scavenging activity of 6-gingerol was determined by electron spin resonance (ESR) spectrophotometer. In this study, JES-FA200 model of X-band ESR (Japan) was used.
Hydroxyl radical scavenging assay
ESR studies were carried out by the method of Morales et al. Briefly, 10 μl of 6-gingerol at various concentrations (10–100 μg/ml) was added to the reaction mixture. Ascorbic acid was used as a reference antioxidant. The ESR spectrum was recorded for every 60 s after the addition of H2O2.
Superoxide anion radical scavenging assay
ESR studies were carried out using Morals et al.'s  method. Briefly, 10 μl of 6-gingerol at various concentrations (10–100 μg/ml) was added to the reaction mixture. SOD was used as a reference antioxidant. The ESR spectrum was recorded for every 120 s after the addition of xanthine oxidase at ambient temperature.
Antiviral activity by in ovo
Komarov mesogenic stain of NDV was obtained from Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India. In our study, 1024 hemagglutination (HA) units of NDV stock was prepared and it was preserved at −40°C until use. Embryonic infective dose (EID50) was evaluated by the method of Reed and Muench  and it was found 109.7 units/ml in chick embryos.
Embryonated chicken eggs
Embryonated chicken eggs were obtained from the Poultry Department, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India.
Inoculation of eggs
After 9–12 days incubation of embryonated eggs, they were used for NDV infection and this was followed by the treatment with 6-gingerol. Eggs were mainly divided into six groups in which 2nd, 4th, 5th, and 6th groups were again divided each into three subgroups (n = 5) based on the inoculation of 6-gingerol concentrations, 25, 50, and 100 μg/ml, respectively. They were perforated and immediately inoculated by the NDV and 6-gingerol as follows: Group-1 eggs were inoculated with 0.1 ml of phosphate buffered saline, Group-2 eggs were inoculated with 6-gingerol, and Group-3 eggs were treated with virus stock (1024 HA units), Group-4, i.e., pretreated group, where eggs were infected with 0.1 ml of stock virus after 1 h inoculation of 0.1 ml 6-gingerol, Group-5, i.e., posttreatment group, where eggs were treated with 6-gingerol after 1 h inoculation of virus, and Group-6 simultaneously treated group where eggs were treated with 0.1 ml of viral stock along with 6-gingerol. Embryo survival rate was observed daily for 5 days. Allantoic fluid was collected from all the groups for HA test to determine the viral titer in the eggs.
HA test was carried out according to OIE manual (1996) standard diagnostic test. HA test was performed and the observations were recorded. All tests were repeated in triplicates.
Data were represented as mean ± standard deviation by Microsoft corporation, Redmond, Washington, United States GraphPad Software, San Diego, California, USA.
| Results and Discussion|| |
Some of the ginger nonvolatile pungent compounds (gingerols, shogaols, and zingerone) have antioxidant, anti-inflammatory, and gastroprotective activity,, and anticancer activity might be due to it's free radical scavenging activity. The phenolic bioactive compounds could reduce the viral growth may due to their antioxidant activity., However, the redox active metals play a major role in the generation of free radicals. In stress condition, excess of superoxide release the iron from iron-containing molecules which generate the highly reactive hydroxyl radicals and make the changes in cell membrane, DNA, and other cellular molecules and which induce the diseases. Therefore, in this study, we investigated the effect of 6-gingerol on free transition metal ion-induced free radical-mediated alterations by in vitro and antiviral activity on NDV by in ovo.
Inhibition of ghost lipid peroxidation by 6-gingerol
Lipid peroxidation is a complex process occurring in cells and it induces the alterations in the membrane and generates potentially toxic products which are covalently interacting with proteins and DNA  bases and damaged. The inhibitory effect of 6-gingerol against ferrous sulfate and ascorbic acid induced ghost lipid peroxidation as shown in [Figure 1]a. 6-gingerol inhibits the lipid peroxidation at high level about 85.28% at low concentration (75 μg/ml) while BHA showed 73.42% at 100 μg/ml. The levels of lipid peroxidation products may use as biomarkers for the measurement of oxidative stress. Our in vitro results suggested that 75 μg/ml concentration of 6-gingerol significantly broke down the chain reaction of lipid peroxidation maybe by donating proton or electrons to the free radicals or chelation of ferrous ions in the Fenton reaction which prevents the generation of free radicals.
|Figure 1: (a) Inhibition of rat red blood cell membrane lipid peroxidation by various concentration of 6-gingerol. (b) Inhibition of DNA sugar damage at various concentrations of 6-gingerol compared with butylated hydroxyanisole. The negative control was without any antioxidant or extract. Results are shown as mean ± standard deviation (n = 3)|
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Inhibitory effect of 6-gingerol on Fenton reactant-induced DNA sugar damage
The results shown in [Figure 1]b exhibit a positive relationship between the concentration of 6-gingerol and inhibition of oxidative DNA sugar damage. The maximum inhibition of DNA sugar damage by 91.26% at 75 μg/ml of 6-gingerol was observed when compared with BHA, which showed 78.30% at 125 μg/ml. Our results suggested that 6-gingerol may have the ability to combat against free radical-mediated degradation of DNA sugar moiety by their antioxidant and metal sequester property.,
Protective effect of 6-gingerol on hydroxyl radical-mediated DNA damage
Increased production of reactive oxygen species concerned to increase the oxidative stress by deterioration of antioxidant mechanism which can lead to proliferate the DNA damage and it has been linked to various pathological disorders. In the present study, calf thymus DNA was subjected to the Fenton reactants for 30 min; as shown in [Figure 2]a, high mobility of DNA (lane-2) was observed on agarose gel when compared with untreated DNA (lane-1) due to extensive DNA fragmentation by hydroxyl radical oxidation. Inhibition of DNA fragmentation of 6-gingerol was observed at various concentrations ranging from 25 to 100 μg/ml. The maximum inhibition of DNA fragmentation was observed at 75 μg/ml (lane 7) which was higher than the known synthetic antioxidant BHA (100 μg/ml) (lane 4). This effectiveness of the 6-gingerol to prevent oxidative DNA damage was concomitant to its hydroxyl radical-scavenging activity. This suggests that 6-gingerol might have the ability to combat free radical-mediated oxidative damage.
|Figure 2: (a) Inhibition of hydroxyl radicals-mediated DNA degradation by 6-gingerol. Lane 1: calf thymus DNA untreated; lane 2: Calf thymus DNA + Fenton reactants lanes 3–7 show the results for calf thymus DNA + Fenton reactants solution + butylated hydroxyanisole (50 μg and 100 μg), 6-gingerol at 25, 50, and 75 μg, respectively. Butylated hydroxyanisole is a standard antioxidant. (b) Cytochrome c reduction capacity of 6-gingerol. The positive control was ascorbic acid. Results are shown as mean ± standard deviation (n = 3)|
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6-Gingerol cytochrome c reduction
In this study, cytochrome c has been used as a model protein. It is an electron transporter in the respiratory chain. 6-gingerol significantly reduced the oxidized cytochrome c in a concentration-dependent manner (25–100 μg/ml). The maximum cytochrome c-reducing capacity of 6-gigenrol was observed at 75 μg/ml which is higher than the known synthetic antioxidant BHA (100 μg/ml) [Figure 2]b. The earlier studies were shown similar results with the ethyl alcohol and water (1:1) extract of curry leaves which contain reducing compounds that reduce ferric-cytochrome c to ferro-cytochrome c. Reduction of cytochrome c by 6-gingerol might also implemented to maintain the cytochrome c redox state which is an important to regulate the apoptosis.
6-Gingerol ferric ion-reducing capacity
It was mainly depended on the substances those have dropping the electrons or hydrogen atoms for reducing the Fe +3/ferricyanide into ferrous form and consequently terminate radical chain reaction. The previous studies have been reported that the extract of spices and herbs showed ferric ion-reducing activity., In the present study, as shown in [Figure 3]a, the maximum reducing capacity of 6-gingerol (1.77 ± 0.10) was observed at lower concentration (75 μg/ml) when compared with BHA at 125 μg/ml concentration (0.912 ± 0.02). The reducing capacity of 6-gingerol might be due to their hydrogen-donating ability.
|Figure 3: (a) Dose-dependent ferric ion-reducing capacity of 6-gingerol. Results are shown as mean ± standard deviation (n = 3). The positive control was butylated hydroxyanisole. (b) Dose-dependent ferrous iron-chelating activity of 6-gingerol. The negative control was without any antioxidant or extract. Results are shown as mean ± standard deviation (n = 3). Positive control was ethylenediaminetetraacetic acid|
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6-Gingerol ferrous ion-chelating activity
Iron is an essential mineral for normal physiology; when it is excess, it facilitates the formation of free radicals via Fenton reaction. The substances those were chelate the iron and removal of free iron from circulation could have valuable antioxidant capability. This can be a promising approach to prevent oxidative stress-induced diseases. Iron pro-oxidant property was last when it is chelated. Hence, we examined the chelation of Fe + 2 by 6-gingerol in a competitive way with potassium ferricyanide. Interestingly, as shown in [Figure 3]b, maximum (80.2%) Fe + 2 binding capability of 6-gingerol was found at 75 μg/ml as evidenced by the low absorption at 700 nm and its chelating effect is comparable with EDTA (50 μg/ml) which showed a chelating effect of 83.93%.
6-Gingerol superoxide radical-scavenging activity
The aerobic animals have able to suppressed superoxide anions by their antioxidant defense system when they are produced in certain limits. However, in excess, they cause cell damage. In the present in vitro study, superoxide anions were generated via nitroblue tetrazolium (NBT) assay system. The results in [Figure 4]a showed superoxide anion-scavenging activity of 6-gingerol at various concentrations ranging from 10 to 100 μg/ml. The maximum superoxide anion-scavenging activity of 6-gingerol (84.96%) was observed at 75 μg/ml while it was 78.27% at 100 μg/ml of BHA. SOD was used as a positive control that showed 87.23% at 25 μg/ml concentration. The active inhibition of the NBT reduction by 6-gingerol suggests that 6-gingerol is a potential scavenger of superoxide anion. The previous studies have also been supported to our results; the ginger significantly scavenges the superoxide anions and acts as a dietary antioxidant in vivo.
|Figure 4: (a) The superoxide-scavenging activity of 6-gingerol. The negative control was without any antioxidant or extract. Positive control superoxide dismutase. (b) Hydroxyl radical-scavenging activity of various concentration of 6-gingerol in comparison with butylated hydroxyanisole. The negative control was without any antioxidant or extract. (c) Inhibition of 1,1-diphenyl-2-picrylhydrazyl radicals at various concentrations of 6-gingerol in comparison with butylated hydroxyanisole. Results are shown as mean ± standard deviation (n = 3)|
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6-Gingerol hydroxyl radical-scavenging activity
Hydroxyl radicals are highly reactive substances and are easily to initiate the cell damage in vivo when compared with other reduced forms of dioxygen. However, from our results [Figure 4]b, 6-gingerol shown maximum hydroxyl radical-scavenging activity by 90.45% at 75 μg/ml which was higher than a well-known synthetic antioxidant BHA (77.25%) at 100 μg/ml. This implies that 6-gingerol may able to reduce the Fe +3–EDTA reactions which was initiated the OH radicals using H2O2. The earlier studies have demonstrated that phenolic compounds showed high antioxidant activity by donating electrons to the free radicals and suppress their activity.
6-Gingerol 1,1-Diphenyl-2-picrylhydrazyl radical-scavenging activity
DPPH analysis is one of the tests used to prove the ability of 6-gingerol to act as donor of hydrogen atoms or electrons. The obtained results shown in [Figure 4]c explain 6-gingerol exhibited a significant effect on inhibition of DPPH radicals in dose-dependent manner. The maximum activity of 6-gingerol (94.66%) was observed at 75 μg/ml and it was higher than the known synthetic antioxidant BHA (79.19%) at 100 μg/ml. The results indicated that 6-gingerol is a powerful free radical scavenger compared with known antioxidant. The hydrogen-donating abilities of the phenolic compounds of the ginger are thought to be responsible for the inhibition of DPPH-free radical.
6-Gingerol hydroxyl radical-scavenging activity by electron spin resonance
The ESR spectrum of DMPO-OH adducts showed a typical 1:2:2:1 quarted line. The ESR signal is proportional to the concentration of spin adducts in the solutions. The ESR signal significantly diminished with increase of 6-gingerol concentrations and it was higher at 75 μg/ml concentration [Figure 5]a,[Figure 5]b,[Figure 5]c,[Figure 5]d,[Figure 5]e,[Figure 5]f,[Figure 5]g when compared with other concentrations. Consequently, this activity was similar to the reference antioxidant ascorbic acid (75 μg/ml). This activity shown by gingerol may be due to (a) suppression of hydroxyl radicals formation by 6-gingerol in the Fenton reaction, (b) donation of proton or electron to radical spin adduct, and (c) competition between 6-gingerol and the spin trap agent for the hydroxyl radical.,
|Figure 5: Quenching effect of various concentrations of 6-gingerol using 5,5'-dimethyl-l-pyrroline-N-oxide to trap the OH radical. Examples of electron paramagnetic resonance spectra signal ranging from 326–346 mT: (a) Control; (b-g) Effects of concentrations ranging from 10 to 75 μg/ml. The positive control was ascorbic acid|
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Superoxide anion radical-scavenging activity of 6-gingerol by electron spin resonance
The results obtained are as shown in [Figure 6]a,[Figure 6]b,[Figure 6]c,[Figure 6]d,[Figure 6]e,[Figure 6]f, the intensity of the signals virtually abolished with increasing of 6-gingerol concentrations from 10 to 100 μg/ml, and it was higher at 75 μg/ml of 6-gingerol concentration when compared with other concentrations. However, this activity was similar with the antioxidant enzyme SOD at 25 μg/ml concentration.
|Figure 6: Quenching effect of various concentrations of 6-gingerol using 5,5'-dimethyl-l-pyrroline-N-oxide to trap the O2 radical. Examples of electron paramagnetic resonance spectra signal ranging from 326 to 346 mT: (a) = control; (b-f) Effects of concentrations ranging from 10 to 75 μg/ml. The positive control was superoxide dismutase|
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In this study, ESR signals of DMPO-OOH were gradually decreased when increased the concentration of 6-gingerol, it may be due to (a) the fact that 6-gingerol inhibits the xanthine oxidase activity and (b) transformation of superoxide anion to oxygen.,
Inhibition of Newcastle disease virus growth in chicken eggs by 6-gingerol
NDV has a single-stranded negative probing filamentous RNA. At present, there is no specific treatment for NDV, and it was only controlled by the vaccination. Recently, there has been a growing interest in plants as natural sources for the treatment of viral diseases as therapeutics.
The results from our in ovo study, as shown in [Table 1], showed that normal control and 6-gingerol alone treated embryonic eggs were not shown any agglutination in HA test and mortality whereas untreated NDV-infected eggs have shown agglutination in HA test (1/1024 virus titer) and all infected eggs died within 48 h. Six-gingerol pretreated viral infected eggs shown 80%, 40%, and 20% mortality rate at concentrations of 25, 50, and 100 μg/ml of 6-gingerol, respectively, whereas in HA test, their viral titer values 1/256, 1/32, and 1/8, respectively. Six gingerol posttreated group eggs showed 100%, 80% and 40% mortality rate at various concentrations of 25, 50, and 100 μg/ml 6-gingerol and their viral titer values were 1/512, 1/128, and 1/32, respectively. Virus and 6-gingerol (1:1 v/v) simultaneously treated group showed 80%, 60%, and 40% mortality rate at various concentrations of 25, 50, and 100 μg/ml of 6-gingerol and their titer values were 1/128, 1/64, and 1/32, respectively.
Several studies made a comparative evaluation of the antioxidant and the antiviral activities of plant extracts and naturals substances. The higher amount of phenolic compounds particularly flavonoids and tannins are known to possess good antiviral activities., In our in ovo study, 6-gingerol pretreatment significantly exerts antiviral activity in a dose-dependent manner which indicates that 6-gingerol inhibited the viral growth. Post and simultaneously 6-gingerol treated NDV-infected eggs have shown low titer value in HA test and mortality when compared with NDV control. This could be suggested that 6-gingerol has ability to prevent the NDV growth at some extent. The antiviral effect of 6-gingerol on NDV might be due to killing the virus and/or interfering with viral multiplication  or may it exhibit protease inhibition, and hence interferes with cleavage of hemagglutinin neuraminidase and fusion protein, which are important to glycoproteins for NDV attachment and multiplication or virucidal effect.,
| Conclusion|| |
Our research results clearly demonstrated that 6-gingerol has shown a good metal sequesters property by recuperation of free transition metal ion-induced free radical alterations by in vitro and it also inhibits the NDV growth in embryonic chicken eggs by in vivo. Thus, this study provides a basis for developing a valuable food additive to protect from the free radical-induced alterations and NDV in chickens
The corresponding author acknowledges the DST-SERB for financial support in the form of DST-SERB/YSS Project (Ref. No. YSS/2014/000945 dated 3/07/2015), a part of work has been carried out with the help of Prof. K. Sathyavelu Reddy, Department of Zoology, S. V. University, in his laboratory out of the grants support received for UGC project (Lr. F. No. 43-586/2014 (SR) dated 30/10/2015), and The Director, Central Instruments Laboratories, Hyderabad Central University, Hyderabad, for providing facilities to carry out the ESR studies.
Financial support and sponsorship
Financial support in the form of DST-SERB/YSS Project (Ref. No.YSS/2014/000945 dated 3/07/2015) and UGC project sanctioned (Lr.F. No. 43-586/2014 (SR) dated 30/10/2015) was obtained.
Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]