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ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 55  |  Page : 161-166

Pharmacokinetic study on piplartine and piperine after oral administration of Piper chaba root by liquid chromatography-mass spectrometry/mass spectrometry


1 Division of Natural Products Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
2 Department of Pharmaceutical Analysis, A.U. College of Pharmaceutical Sciences, Andhra University, Vishakapatnam, Andhra Pradesh, India
3 Division of Medicinal Chemistry and Biotechnology, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India

Correspondence Address:
Suresh Babu Katragadda
Division of Natural Products Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad - 500 007, Andhra Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_470_17

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Background: Piperaceae family are a well-known source of structurally diverse amides with the wide range of bioactivities such as cytotoxic, stomach aches, insect repellents, anti-inflammatory, insecticidal, and antifeedant activities. It has been reported that piplartine and piperine, alkaloid/amide compounds from Piper species, show antitumor activities. Objective: A rapid, sensitive liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of piplartine and piperine from Piper chaba extract. Materials and Methods: The two analytes, together with internal standard (IS, trichostachine), were separated on a Waters Acquity ethylene bridged hybrid C18(2.1 mm × 100 mm, 1.9 μ) column using a mobile phase of acetonitrile with 0.1% formic acid and water with 0.1% formic acid (70:30, v/v) with isocratic elution. The detection was performed using the positive ion electrospray ionization in multiple reaction monitoring mode with transitions at m/z 318→221 for piplartine, m/z 286→201 for piperine, and m/z 272→201 for the IS. Results: The calibration curves were both linear (r2 > 0.995) over a concentration range of 1.0–2000 ng/mL; the lower limit of detection quantification was 1.0 ng/mL for both piplartine and piperine. The intra-day and inter-day precisions (relative standard deviation %) were <10.9%, and recoveries ranged from 90.3% to 103.0%. Conclusions: The analytes were proven stable in the short-term, long-term, and after three freeze-thaw cycles. The method was successfully applied to pharmacokinetic studies of piplartine and piperine in rats after oral administration of P. chaba extract. Abbreviations used: AUC: Area under the curve; BEH: Ethylene bridged hybrid; CDER: Centre for drug evaluation and research; CID: Collision-induced dissociation; Cmax: Maximum concentration; CTO: Column Temperature Oven; DGU: Degassing Unit; ESI: Electrospray ionization; eV: Electron volt; FCV: Flow control valve; HPLC: High-pressure liquid chromatography; HPTLC: High performance thin layer chromatography; IS: Internal standard; LLOQ: Lower limit of quantitation; LC: Liquid chromatography; LC-MS: Liquid chromatography-Mass Spectrometry; LC-MS/MS: Liquid chromatography-Mass Spectrometry/Mass Spectrometry; LC-HRMS: Liquid chromatography-High resolution mass Spectrometry; LC-NMR-MS: Liquid chromatography-Nuclear magnetic resonance-Mass Spectrometry; MRM: Multiple reaction monitoring; MC: Methyl cellulose; N2: Nitrogen; RSD: Relative standard deviation; RE: Relative error; r2: Regression coefficient; t1/2: Half-life; Tmax: Time to maximum effect; QC: Quality control; UFLC: Ultrafast liquid chromatography; UPLC-qTOF-MS: Ultra pressure liquid chromatography-Time of flight-Mass spectrometry; USFDA: United states Food and Drug Administration


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