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ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 54  |  Page : 248-252

Rapid screening and characterization of acetylcholinesterase inhibitors from Yinhuang oral liquid using ultrafiltration-liquid chromatography-electrospray ionization tandem mass spectrometry


1 College of Acupuncture and Massage, Changchun University of Chinese Medicine, Changchun, PR China
2 The Affiliated Hospital of Changchun University of Traditional Medicine, Changchun University of Chinese Medicine, Changchun, PR China
3 Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun, PR China
4 Pharmacy College, Changchun University of Chinese Medicine, Changchun, PR China

Correspondence Address:
Jiaming Sun
Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun
PR China
Ying Gao
Pharmacy College, Changchun University of Chinese Medicine, Changchun
PR China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_166_17

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Background: At present, approximately 17–25 million people in the world suffer from Alzheimer's disease (AD). The most efficacious and acceptabletherapeutic drug clinically are the acetylcholinesterase inhibitors (AChEIs). Yinhuang oral liquid is a Chinese medicine preparation which contains AChEIs according to the literatures. However, no strategy has been presented for rapid screening and identification of AChEIs from Yinhuang oral liquid. Objective: To develop a method for rapid screening and identification of AChEIs from Yinhuang oral liquid using ultrafiltration–liquid chromatography–electrospray ionization tandem mass spectrometry (UF-LC-ESI-MS/MS). Materials and Methods: In this study, UF incubation conditions such as enzyme concentration, incubation time, and incubation temperature were optimized so as to get better screening results. The AChEIs from Yinhuang oral liquid were identified by high-performance liquid chromatography-ESI-MS and the improved Ellman method was used for the AChE inhibitory activity test in vitro. Results: The results showed that Yinhuang oral liquid can inhibit the activity of AChE. We screened and identified seven compounds with potential AChE inhibitory activity from Yinhuang oral liquid, which provided experimental basis for the treatment and prevention of AD. Conclusion: The current technique was used to directly screen the active ingredients with acetylcholinesterase inhibition from complex traditional Chinese medicine, which was simple, rapid, accurate, and suitable for high-throughput screening of AChEI from complex systems. Abbreviations used: (AD): Alzheimer's disease; (UF-LC-ESI-MS/MS): ultrafiltration–liquid chromatography–electrospray ionization tandem mass spectrometry; (AChEIs): acetylcholinesterase inhibitors.


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