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ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 54  |  Page : 220-226

Combination of Garcinia cambogia extract and pear pomace extract additively suppresses adipogenesis and enhances lipolysis in 3T3-L1 cells


1 College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-Gun, Republic of Korea
2 Jeonnam Nano Bio Research Center, Jangseong-gun, Jeollanam-Do, Republic of Korea

Correspondence Address:
Eunyoung Yi
College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, 1666 Yeongsan-Ro, Cheonggye-Myeon, Muan-gun, Jeollanam.do
Republic of Korea
Min-Ho Oak
College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, 1666 Yeongsan-Ro, Cheonggye-Myeon, Muan-gun, Jeollanam-do
Republic of Korea
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_388_17

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Background: Inhibition of adipogenesis has been a therapeutic target for reducing obesity and obesity-related disorders such as diabetes, hypertension, atherosclerosis, and cancer. For decades, anti-adipogenic potential of many herbal extracts has been investigated. One example is Garcinia cambogia extract (GE) containing (-)-hydroxycitric acid as an active ingredient. GE is currently marketed as a weight loss supplement, used alone or with other ingredients. Pear pomace extract (PE), another natural product, has been also shown to have anti-adipogenic activity in a recent report. Objective: It was tested if the mixture of PE and GE (MIX) would produce more effective anti-adipogenic activity than PE or GE alone. Materials and Methods: Differentiation of 3T3-L1 preadipocyte was induced by adding insulin, dexamethasone, and isobutylmethylxanthine and lipid accumulation was measured by Oil Red O staining. Cellular markers for adipogenesis and lipolysis such as CCAAT/enhancer binding protein (C/EBP-α), peroxisome proliferator-activated receptor gamma (PPAR-γ), fatty acid synthase (FAS), and hormone-sensitive lipase (HSL) was measured using immunocytochemistry. Results: MIX, compared to PE or GE alone, showed greater inhibition of lipid accumulation. Furthermore, MIX reduced the expression of adipogenesis-related factors C/EBP-α, PPAR-γ, and FAS more than PE or GE alone did. In contrast, the expression of HSL the enzyme required for lipolysis was further enhanced in MIX-treated adipocytes compared to the PE or GE alone treated groups. Conclusions: Anti-adipogenic effect of PE and GE appears synergistic, and the MIX may be a useful therapeutic combination for the treatment of obesity and obesity-related diseases. Abbreviations used: CEBP-a: CCAT/enhancer binding protein alpha, CI: Combination Index, FAS: Fatty acid synthase, GE: Garcinia cambogia extract, HSL: Hormone sensitive lipase, PE: Pear pomace extract, PPAR-γ: Peroxisome proliferator-activated receptor gamma.


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