|Year : 2018 | Volume
| Issue : 54 | Page : 207-213
Evidence for the involvement of COX-2/VEGF and PTEN/Pl3K/AKT pathway the mechanism of oroxin B treated liver cancer
Nan-Nan Li1, Xian-Sheng Meng2, Yong-Rui Bao2, Shuai Wang2, Tian-Jiao Li2
1 School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
2 School of Pharmacy, Liaoning University of Traditional Chinese Medicine; Component Medicine Engineering Research Center of Liaoning Province; Liaoning Province Modern Chinese Medicine Research Engineering Laboratory, Dalian 116600, China
|Date of Submission||23-Mar-2017|
|Date of Acceptance||13-Jun-2017|
|Date of Web Publication||10-Apr-2018|
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 18 of Dd5 Street, Dalian, Liaoning Province 116600
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Oroxin B (OB) is one of flavonoids isolated from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent. Recent studies suggest that flavonoids have obvious anti-liver tumors effect, but the precise molecular mechanism is still unclear. Objective: The current study was performed to investigate the antitumor effects of OB on human hepatoma cell line SMMC-772 and explore the part of molecular mechanisms in this process. Materials and Methods: MTT method, terminal deoxynucleotidyl transferase dUTP nick end labeling assay and flow cytometry were utilized to detect the inhibition of proliferation and the apoptosis after treating OB in of SMMC-7721 cells. The mRNA and proteins expressions of COX-2, vascular endothelial growth factor (VEGF), phosphatidylinositol-3-kinase (PI3K), p-AKT, and PTEN were measured by a real-time polymerase chain reaction and Western Blot method. Results: The results showed that OB inhibited proliferation of SMMC-7721 cell in a dose-dependent manner, and induced its apoptosis. Moreover, OB unregulated PTEN and downregulated COX-2, VEGF, p-AKT, and PI3K. Conclusion: Our results demonstrated that OB significantly inhibits proliferation and induce apoptosis, which may be strongly associated with the inhibiting COX-2/VEGF and PTEN/PI3K/AKT pathway signaling pathway in SMMC-7721 cells, OB potentially be used as a novel therapeutic agent for liver cancer.
Abbreviations used: OB: Oroxin B; MTT: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide; COX-2: cyclooxygenase-2; PI3K: phosphatidylinositol 3 kinase; PTEN: Phosphatase and tensin homolog deleted on chromosome ten; VEGF: Vascular endothelial growth factor; RT-PCR: Reverse transcription polymerase chain reaction; DAPI: Diamidino 2 phenylindole; PBS: Phosphate buffer saline; FITC: Fluorescein isothiocyanate; PI: Propidium Iodide; RIPA: Radio immunoprecipitation assay lysis buffer; PMSF: Phenylmethanesulfonyl fluoride; PAGE: Polyacrylamide gel electrophoresis.
Keywords: Anti-liver cancer, COX-2/vascular endothelial growth factor, Oroxin B, PTEN/phosphatidylinositol-3-kinase/AKT, SMMC-7721 cells
|How to cite this article:|
Li NN, Meng XS, Bao YR, Wang S, Li TJ. Evidence for the involvement of COX-2/VEGF and PTEN/Pl3K/AKT pathway the mechanism of oroxin B treated liver cancer. Phcog Mag 2018;14:207-13
|How to cite this URL:|
Li NN, Meng XS, Bao YR, Wang S, Li TJ. Evidence for the involvement of COX-2/VEGF and PTEN/Pl3K/AKT pathway the mechanism of oroxin B treated liver cancer. Phcog Mag [serial online] 2018 [cited 2019 Jul 23];14:207-13. Available from: http://www.phcog.com/text.asp?2018/14/54/207/229675
- OB (Oroxin B) is one of the effective flavonoid components of traditional Chinese medicine O. indicum (L.)
- OB can inhibite the proliferation and promoted apoptosis of the human hepatoma cell line SMMC 7721
- OB plays a role of antitumor effect may to regulate COX 2/VEGF and PTEN/PI3K/AKT pathways directly or indirectly.
| Introduction|| |
Liver cancer is the common malignant tumor, cause of cancer deaths due to its high incidence and the lack of effective treatments. Now the clinical use of chemotherapy drugs has a lot of side effects for patients, such as liver and kidney toxicity, immunosuppressive effect, and drug resistance. For treating liver cancer, it is significant to look for effective and low toxic drugs, investigate the molecular mechanisms of liver cancer pathogenesis, and develop beneficial therapeutic treatments.
Modern research has shown that the antitumor action of medicine might be attributed to inducing cell apoptosis, inhibiting tumor angiogenesis, adjusting the cell signal transduction, regulating the body's immune function, etc., Moreover, numbers of targets and pathways have been proposed to taking part in the antitumor mechanisms, such as COX-2/vascular endothelial growth factor (VEGF) and PTEN/phosphatidylinositol 3-kinase (PI3K)/AKT pathway.,, COX-2/VEGF pathway is an important pathway related to tumor angiogenesis. COX-2, which is highly induced in response to cell apoptosis, tumor invasiveness, and angiogenesis, is a rate-limiting enzyme and have been found overexpression in varieties of cancers., VEGF as a pro-angiogenic factor is increased in many tumor types is associating with tumor vascularity and tumor invasiveness. Recently, research in cancer cells suggests that overexpression of COX-2, which play roles in regulating angiogenesis by modulating the production of many angiogenic factors, including VEGF, is along with growing tumor., In this study, the expression of VEGF and COX-2 was investigated after OB was treated with liver cancer SMMC-7721 cells. The antitumor mechanism was illustrated.
PI3K/AKT signaling pathways is an important metabolic pathway concerning to apoptosis in vivo. PI3K, which is frequently over-activated in human cancers, including hepatoma cancer, is a fundamental enzyme involved in cell growth and proliferation., The signaling is naturally antagonized by the tumor suppressor gene PTEN, PTEN which is a phosphatase that terminates the signaling of PI3K/AKT pathway by dephosphorylating PIP3, PTEN inhibited PI3K and AKT abnormal activation, and stop all downstream regulated by AKT signaling, thus inhibiting tumor cell proliferation and promote tumor cell apoptosis, and play a role for the treatment of liver tumors.,,,,,, In our research, OB effect on liver cancer cell apoptosis and its mechanisms were studied by flow cytometry instrument, reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
Oroxin B (OB, Baicalein7-O-β-gentiobioside, C27H30O15, MW 594.52 Da) is one of the flavonoids isolated from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent. Flavones have been approved to be effective constituents in O. indicum (L.) Vent., Recent studies suggest that flavonoids possess antitumor, anti-inflammatory activities, etc., Although the current research shows that flavones have obvious anti liver tumors effect, the precise molecular mechanism is still unclarified. Accordingly, systematic study on the anti-liver cancer effect and molecular mechanisms of OB are particularly important for the development of new therapeutic strategies. In this research, the anti-liver tumors effects of OB using in vitro (human liver cancer SMMC-7721 cells) models were explored. Our results demonstrated that OB possessed obvious inhibitory effect and induced cell apoptosis on liver cancer cells in vitro through upregulation of PTEN, downregulation of COX-2, VEGF, PI3K, and p-AKT. This experiment was based on related genes and proteins to interpret the mechanism of antitumor effect on SMMC-7721 cells after treated with OB. Proving that OB possessed treatment of liver cancer by taking part in multiple metabolic pathways and targets directly or indirectly, such as PI3K-Akt signaling pathway, and COX-2/VEGF signaling pathway.
| Materials and Methods|| |
The human hepatoma cell line SMMC-7721 was supplied by the Shanghai Cell Bank, Cell Bank, Chinese Academy of Science (Shanghai, China). This cell line was culture in RPMI 1640 medium (Gibco, GrandIsland, USA) supplemented with 10% fetal bovine serum (Gibco, GrandIsland, USA) and 1% penicillin/streptomycin (Gibco, GrandIsland, USA) at 37°C in a humidified incubator containing 5% CO2.
Cell viability was determined through MTT assays. Briefly, cells were plated in 96-well plates at a density of 1 × 104 per well. After overnight culture, different concentrations of OB (0.34, 1.01, 1.68 μM) were added to the wells and cells were incubated for 48 h. After drug treatment, the culture medium was replaced with MTT (Sigma St. Louis, MO, USA) fresh serum-free medium (final concentration, 2.5 mg/ml), followed by a 4 h incubation. In addition, 150 μl of dimethyl sulfoxide (Sigma St. Louis, MO, USA) was added to each well. Absorbance was measured with a Spectra Max Plus microplate reader (Molecular Devices, CA, USA) at a wavelength of 492 nm.
The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed according to the manufacturer's instructions. The cells were treated with OB for 12 h, then 1% paraformaldehyde in PBS fixed 30 min, 0.2% Triton X-100 in PBS permeabilized for 5 min, rinsed with PBS, and incubated with PE buffer (10 mM Tris–HCl, pH 7.5, 80% ethanol) at 37°C for 1 h. Then, they were stained with 4',6-diamidino-2-phenylindole (DAPI; Sigma, St. Louis, MO, USA) at 37°C for 5 min. Finally, fluorescence was recorded on an inverted fluorescence microscope (Nikon Eclipse Ti). The flow cytometer was used FITC/PI double-staining method, each group cells including treated cells (cells were treated with OB for 48 h) were collected and rinsed twice with cold PBS, mixed with 500 μl of 1 × binding buffer (1 × 106/ml), 5 μl Annexin V-FITC, 5 μl propidium iodide, and incubated in the dark for 15 min and finally sent to the BD Accuri C6 flow cytometer to analyze the cell apoptosis.
RNA isolation and real-time quantitative polymerase chain reaction analysis
Total RNA of each group cells was extracted using TRIzol (Ambion, Texa, USA) according to the manufacturer's recommendations. Total RNA was reverse-transcribed using the TransScript First-Strand cDNA Synthesis SuperMix (TransGen Biotech, China). Quantitative real-time PCR was performed using TransStart Top Green qPCR SuperMix (TransGen Biotech, China) with Piko Thermal Cycler 96-well system (Piko, Hawaii State, USA). Each sample was analyzed in triplicate. Relative levels of mRNA expression were normalized for β-actin mRNA expression and calculated according to the formula 2−(ΔCtsample−ΔCtcontrol). Primer sequences for the genes: h-PTEN F: 5'-CCCAGTCAGAGGCGCTATG-3', R: 5'-GGCAGACCACAAACT GAGGATT-3'; h-COX2 F: 5'-AGCAGGCAGATGAAAT ACCAGTCT-3', R: 5'-ATACAGCTCCA CAGCATCGATGT-3'; h-pAKT F: 5'-GGAGGAGATCACTGGCAA-3', R: 5'-CATGGTCAGTGT GCCTCTCA-3'; h-PI3K F: 5'-AACGAGA ACGTGT GCCATTTG-3', R: 5'-AGAGATTGGCATG CTGTCGAA-3'; h-VEGF F: 5'-AGGAGTACC CTGATGAGATCGAGTA-3', R: 5'-TGGTGAGGTTTG ATCCGCATA; β-actin F: 5'-CACCCGCGAGTACAACCTTC-3', R: 5'-CCCA TACCCACCATCACACC-3' (Invitrogen, USA). All primers final concentration is 100 μM/l. The reaction system and reaction time are shown in [Table 1], [Table 2], [Table 3], [Table 4].
|Table 3: Reverse transcription polymerase chain reaction synthesis system|
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|Table 4: Reverse transcription polymerase chain reaction synthesis conditions|
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Western blotting analysis
Protein extracts were isolated from each group cells in using RIPA protein lysis buffer containing 1mM PMSF. Total protein was separated by 10% SDS-PAGE, transferred with polyvinylidene difluoride membrane, blocked in 5% BSA (Solarbio, Beijing, China) and probed with appropriate primary antibodies against the target proteins, VEGF antibody was purchased from Abcam (Santa Cruz, USA). PTEN, PI3 Kinase p85, p-AKT, COX-2, and β-actin antibodies were purchased from Cell Signaling (Beverly, MA, USA). These were followed by incubation with Goat Anti-Rabbit immunoglobulin G (H + L) (Protein tech, USA), and antigen-antibody complexes were visualized using the chemilucent ECL (TransGen Biotech, China) detection system.
Statistical analysis was performed using t-test or one-way ANOVA with GraphPad Prism 5. The Pvalues were considered statistically significant at P < 0.05. All data are means ± standard deviation for at least three separate experiments.
| Results|| |
Oroxin B inhibits the proliferation in the human hepatoma cell line SMMC-7721
To investigate the effect of OB on the proliferation of human hepatoma cell line SMMC-7721, we set up different concentrations of OB, 0.34, 1.01, and 1.68 μM then continued to incubate for 48 h. The results showed that each concentration has obvious proliferation inhibition on hepatoma cancer SMMC-7721cells in vitro, and the inhibition rate increased with the increase of concentration, it indicated that OB can inhibited the proliferation of SMMC-7721 cells in a dose-dependent manner [Figure 1].
|Figure 1:Oroxin B inhibits the proliferation in the human hepatoma cell line SMMC-7721 (n = 3). X-axis is the inhibition ratio of SMMC-7721 cells, and y-axis is the different concentrations of Oroxin B (0.34, 1.01, and 1.68 μM), **P < 0.01 versus control group|
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Oroxin B induces apoptosis in the human hepatoma cell line SMMC-7721
To identify the effect of OB on apoptosis in SMMC-7721 cells, Annexin V-FITC/PI double fluorescence staining method and TUNEL assay were used. The results of the TUNEL assay, the proportion of apoptotic cells was calculated as the percentage of red cells relative to the total DAPI-positive cells. The statistical analysis showed that the rate of apoptosis in OB group was 21.55% ± 2.58%, (P < 0.01 compared with the Control group, [Figure 2]c). The rate of apoptosis in positive group was 25.13% ± 2.74%, (P < 0.01 compared with the Control group, [Figure 2]c), but no significant difference was found between OB and positive groups. In the flow cytometry analysis, the statistical analysis showed that the rate of apoptosis in OB group was 43.03% ± 3.07%, (P < 0.01 compared with the Control group, [Figure 2]c), the statistical analysis showed that the rate of apoptosis in positive group was 58.87% ± 4.86%, (P < 0.01 compared with the Control group, [Figure 2]c), and the difference between two groups was that OB can induce early stage of apoptosis clearly, and positive group can induce late apoptosis, and difference from the control group was obvious [Figure 2]b.
|Figure 2: The effect of Oroxin B on cell apoptosis. (a) Cell apoptosis profiles by terminal deoxynucleotidyl transferase dUTP nick end labeling assay of SMMC-7721 cells treated with Oroxin B (1.68 μM) and Cisplatin (positive control group) for 12 h. (b) cell apoptosis profiles of SMMC-772 cells, treated with Oroxin B (1.68 μM) and Cisplatin for 48 h, were subjected to Annexin-V-FITC/PI binding and analyzed by flow cytometry. (c) Statistical analysis results of cell apoptosis ratio by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. (d) Statistical analysis results of cell apoptosis ratio by flow cytometry. **P < 0.01 versus control group|
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Effect of Oroxin B on COX-2, vascular endothelial growth factor, p-Akt, phosphatidylinositol 3-kinase, PTEN mRNA, and protein expression in SMMC-7721 cells
To explore the OB's influence on the COX-2/VEGF and PI3K/AKT signaling pathway, RT-PCR method was used to study the effects on the expression of COX-2, VEGF, PI3K, p-AKT, and PTEN mRNA in SMMC-7721 cells when treated with OB. We observed that COX-2, VEGF, PI3K, and p-AKT mRNA high expression in SMMC-7721 cells, but the levels were greatly decreased when treated with OB [Figure 3]. We also found that the expression of PTEN mRNA a was increased compared with control group (**P < 0.01) after being treated by OB. Western blot analyses were further confirmed that whether lower levels of COX-2, VEGF, p-AKT, PI3K protein and higher levels of PTEN were observed in the OB-treated cells. As shown in the [Figure 4], OB downregulated COX-2, VEGF, PI3K, and p-AKT protein's expression obviously when compared with Control group *P < 0.05 [Figure 4]b, [Figure 4]c, [Figure 4]d, [Figure 4]e, the expression of PTEN protein is significantly higher in OB-treated cells when compared to the control group (**P < 0.01) [Figure 4]f. Western blot results consistent with the results of RT-PCR.
|Figure 3: The effect of OB on COX-2, VEGF, p-Akt, PI3K, and PTEN mRNA expression in SMMC-7721 cells. mRNA expression levels of COX-2, VEGF, PI3K, p-Akt, and PTEN in SMMC-7721 cells following OB treatment for 48 h. The mRNA levels of mRNAs were detected by real-time PCR and measured using Piko Real Software with β-actin as an internal reference. All data, repeated by three independent experiments, are presented as mean ± standard deviation. *P < 0.05 and **P < 0.01 versus control group. (a) The expression of COX-2, VEGF, PI3K, p-Akt, and PTEN mRNA in SMMC-7721 cells. (b) Expression of COX-2 in each group. (c )Expression of VEGF in each group. (d) Expression of p-AKT in each group. (e )Expression of PI3K in each group. (f ) Expression of PTEN in each group. VEGF: Vascular endothelial growth factor; PI3K: Phosphatidylinositol-3-kinase; OB: Oroxin B|
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|Figure 4: The effect of OB on COX-2, VEGF, p-Akt, PI3K, PTEN protein expression in SMMC-7721 cells. Protein expression levels of COX-2, VEGF, PI3K, p-Akt, and PTEN in SMMC-7721 cells following OB treatment for 48 h. The protein levels were detected by Western blotting analyses. All data, repeated by three-independent experiments, are presented as mean ± standard deviation. *P < 0.05 and **P < 0.01 versus control group. (a) Western blotting analyses of COX-2, VEGF, PI3K, p-Akt, and PTEN protein expression in SMMC-7721 cells. (b) COX-2 protein expression in cells. (c) VEGF protein expression in cells. (d) PI3K protein expression in cells. (e) p-Akt protein expression in cells. (f) PTEN protein expression in cells. VEGF: Vascular endothelial growth factor; PI3K: Phosphatidylinositol 3-kinase; OB: Oroxin B|
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| Discussion|| |
OB, monomer composition, is a flavonoid compound originated from traditional Chinese medicine O. indicum (L.). Recent studies have suggested that flavonoids in O. indicum (L.) are beneficial to treat liver cancer, but the mechanisms of their antitumor activity remain incompletely understood. In this research, OB treatment possessed obvious inhibition of the proliferation in the human liver cancer SMMC-7721 cells, and the inhibition was enhanced with the increasing dose. The result of TUNEL assay showed that, OB-induced cell apoptosis in the human liver cancer SMMC-7721 cells [Figure 2]. We selected cisplatin as positive control which is a conventional chemotherapy drug used in clinical lines when defensed against tumors, especially lung and hepatoma tumor. The flow cytometry results showed that cisplatin could obviously induce late apoptosis and OB-induced early apoptosis of SMMC-7721 cells [Figure 3] and [Figure 4]. Compared with cisplatin, OB had a good curative effect, could induce early apoptosis rather than late apoptosis, which laid the foundation for us to study on related targets. Therefore, OB as an antitumor drug had great value for further development.
For investigating the potential mechanism of OB antitumor effects, we evaluated the regulating effect of OB on COX-2, VEGF, PI3K, AKT, and PTEN expression in the human liver cancer SMMC-7721 cells. Recent research suggests that highly expressed COX-2 and VEGF indicates a poor prognosis, and overexpression of COX-2 in the growth and diffusion of tumor is triggered by its regulation of several angiogenic factors, such as VEGF,, which is widely accepted as a primary inducer of angiogenesis.
In many physiologic and pathologic processes, angiogenesis is the basis of chronic inflammation, ischemic disease and cancer. It is a multistep process occurring in preexisting blood vessels, including newly blood vessels migration, extracellular matrix remodeling, and endothelial cell proliferation and migration., The results of the present study, COX-2 and VEGF expression were decreased significantly after treated with OB [Figure 4], it suggested that OB could play a potential role for treating liver cancer through the regulation of COX-2/VEGF signaling pathway, cutting off the blood supply a neoplastic lesion could be potentially starved into remission, then indirectly play antitumor effect.
PI3K/AKT signaling participates in multiple signaling pathways which modulate cell proliferation, differentiation, migration, and apoptosis. PI3K is located at the cell membrane; when it is activated by a number of receptor tyrosine kinases (such as VEGFR), it initiates the signaling cascade through phosphorylation, leads PIP2 into PIP3. However, the role of PTEN is just the opposite., PTEN has been identified to participate in the PI3K/AKT apoptotic pathways and related to cellular apoptosis or cell proliferation. Therefore, PTEN, PI3K, and AKT have been demonstrated to be the targets for intervening liver cancer.,,,, Our study proved that OB could down regulate VEGF, PI3K, and p-AKT both in mRNA and protein level, meanwhile, it could upregulate the expression of PTEN mRNA and protein [Figure 4]. This study indicated that OB could decrease VEGF protein expression to reduce the activation of PI3K, thus inactive the PI3K/AKT signaling pathway. It could also be concluded that PTEN served as a negative regulatory factor in the PI3K/AKT signaling pathway, thus inhibiting the abnormal activation of PI3K and AKT could contribute to cellular apoptosis thus play a role of antitumor [Figure 5]. The results suggested that OB was participated in PI3K/AKT signaling pathways, influenced the expression of VEGF, PTEN, PI3K, and p-AKT, thus induced the apoptosis and proliferation of cancer cell.
|Figure 5: The relationship between VEGF and PI3K/Akt/PTEN signaling pathways. VEGF: Vascular endothelial growth factor; PI3K: Phosphatidylinositol 3-kinase|
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In conclusion, our findings revealed that OB effectively inhibited proliferation, and it also could induce early apoptosis of human liver cancer cells. COX-2, VEGF, PI3K, and p-AKT expression levels were found to be downregulated, while PTEN was upregulated after OB treatment. We could infer that OB inhibited cell proliferation and reduced angiogenesis partly through regulation of COX-2/VEGF pathways, and OB induced cancer cell apoptosis may relate to PTEN/PI3K/AKT pathways directly or indirectly. Thus, we can infer that OB plays a role of antitumor effect may to regulate these targets, and this paper laid the foundation of OB as antitumor targets drugs development and the research of antitumor mechanism. In addition, for developing nontoxic OB as a potential of liver cancer, clinical trials will be necessary.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin 2012;62:10-29.
Mierlo KM, Schaap FG, Dejong CH, Olde Damink SW. Liver resection for cancer: New developments in prediction, prevention and management of postresectional liver failure. J Hepatol 2016;16:30268-9.
He J, Liang X, Luo F, Chen X, Xu X, Wang F, et al.
P53 is involved in a three-dimensional architecture-mediated decrease in chemosensitivity in colon cancer. J Cancer 2016;7:900-9.
Jiang Y, Xu H, Wang J. Alantolactone induces apoptosis of human cervical cancer cells via reactive oxygen species generation, glutathione depletion and inhibition of the Bcl-2/Bax signaling pathway. Oncol Lett 2016;11:4203-7.
Kuramochi H, Nakamura A, Nakajima G, Kaneko Y, Araida T, Yamamoto M, et al.
PTEN mRNA expression is less pronounced in left- than right-sided colon cancer: A retrospective observational study. BMC Cancer 2016;16:366.
Liu Y, Cao Y, Sun S, Zhu J, Gao S, Pang J, et al.
Transforming growth factor-beta1 upregulation triggers pulmonary artery smooth muscle cell proliferation and apoptosis imbalance in rats with hypoxic pulmonary hypertension via the PTEN/AKT pathways. Int J Biochem Cell Biol 2016;77:141-54.
Xu CQ, Liu BJ, Wu JF, Xu YC, Duan XH, Cao YX, et al.
Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt and NF-kappaB signaling pathway. Eur J Pharmacol 2010;642:146-53.
Setia S, Nehru B, Sanyal SN. The PI3K/Akt pathway in colitis associated colon cancer and its chemoprevention with celecoxib, a Cox-2 selective inhibitor. Biomed Pharmacother 2014;68:721-7.
Millanta F, Andreani G, Rocchigiani G, Lorenzi D, Poli A. Correlation between cyclo-oxygenase-2 and vascular endothelial growth factor expression in canine and feline squamous cell carcinomas. J Comp Pathol 2016;154:297-303.
Mohammed SI, Khan KN, Sellers RS, Hayek MG, DeNicola DB, Wu L, et al.
Expression of cyclooxygenase-1 and 2 in naturally-occurring canine cancer. Prostaglandins Leukot Essent Fatty Acids 2004;70:479-83.
Liu N, Huang J, Sun S, Zhou Z, Zhang J, Gao F, et al.
Expression of matrix metalloproteinase-9, cyclooxygenase-2 and vascular endothelial growth factor are increased in gastrointestinal stromal tumors. Int J Clin Exp Med 2015;8:6495-501.
Peng N, Gao S, Guo X, Wang G, Cheng C, Li M, et al.
Silencing of VEGF inhibits human osteosarcoma angiogenesis and promotes cell apoptosis via VEGF/PI3K/AKT signaling pathway. Am J Transl Res 2016;8:1005-15.
Jung I, Gurzu S, Turdean S, Ciortea D, Sahlean DI, Golea M, et al.
Relationship of endothelial area with VEGF-A, COX-2, maspin, c-KIT, and DOG-1 immunoreactivity in liposarcomas versus non-lipomatous soft tissue tumors. Int J Clin Exp Pathol 2015;8:1776-82.
Wang T, Gong X, Jiang R, Li H, Du W, Kuang G, et al.
Ferulic acid inhibits proliferation and promotes apoptosis via blockage of PI3K/Akt pathway in osteosarcoma cell. Am J Transl Res 2016;8:968-80.
Cianciulli A, Calvello R, Porro C, Trotta T, Salvatore R, Panaro MA, et al.
PI3k/Akt signalling pathway plays a crucial role in the anti-inflammatory effects of curcumin in LPS-activated microglia. Int Immunopharmacol 2016;36:282-90.
Sun GW, Qiu ZD, Wang WN, Sui X, Sui DJ. Flavonoids extraction from propolis attenuates pathological cardiac hypertrophy through PI3K/AKT signaling pathway. Evid Based Complement Alternat Med 2016;2016:6281376.
Daragmeh J, Barriah W, Saad B, Zaid H. Analysis of PI3K pathway components in human cancers. Oncol Lett 2016;11:2913-8.
Mao Y, Xi L, Li Q, Cai Z, Lai Y, Zhang X, et al.
Regulation of cell apoptosis and proliferation in pancreatic cancer through PI3K/Akt pathway via polo-like kinase 1. Oncol Rep 2016;36:49-56.
Qiu FN, Huang Y, Chen DY, Li F, Wu YA, Wu WB, et al.
Eukaryotic elongation factor-1α 2 knockdown inhibits hepatocarcinogenesis by suppressing PI3K/Akt/NF-κB signaling. World J Gastroenterol 2016;22:4226-37.
Li YN, Zhou YZ, Zhang N, Wang HW. Expression of phosphatase and tensin homology deleted on chromosometen (PTEN) in squamous-cell lung cancer and its clinical significance. Zhonghua Jie He He Hu Xi Za Zhi 2016;39:450-3.
Chen LJ, Games DE, Jones J. Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum
by high-speed counter-current chromatography. J Chromatogr A 2003;988:95-105.
Mat Ali R, Houghton PJ, Raman A, Hoult JR. Antimicrobial and antiinflammatory activities of extracts and constituents of Oroxylum indicum
(L.) Vent. Phytomedicine 1998;5:375-81.
Zhai XY, Xiao W, Yang B, Meng ZQ, Wang ZZ, Huang WZ, et al.
Study on chemical constituents from seed of Oroxylum indicum
. Zhongguo Zhong Yao Za Zhi 2015;40:3013-6.
Wei XN, Lin BB, Xie GY, Li JW, Qin MJ. Chemical constitunents of seeds of Oroxylum indicum
. Zhongguo Zhong Yao Za Zhi 2013;38:204-7.
Deori K, Yadav AK. Anthelmintic effects of Oroxylum indicum
stem bark extract on juvenile and adult stages of Hymenolepis diminuta
(Cestoda), an in vitro
and in vivo
study. Parasitol Res 2016;115:1275-85.
Singh J, Kakkar P. Oroxylin A, a constituent of Oroxylum indicum
inhibits adipogenesis and induces apoptosis in 3T3-L1 cells. Phytomedicine 2014;21:1733-41.
Yang P, Fu S, Cao Z, Liao H, Huo Z, Pan Y, et al.
Oroxin B selectively induces tumor-suppressive ER stress and concurrently inhibits tumor-adaptive ER stress in B-lymphoma cells for effective anti-lymphoma therapy. Toxicol Appl Pharmacol 2015;288:269-79.
Yang S, Gao Q, Jiang W. Relationship between tumour angiogenesis and expression of cyclo-oxygenase-2 and vascular endothelial growth factor-A in human renal cell carcinoma. J Int Med Res 2015;43:110-7.
Labanca V, Bertolini F. A combinatorial investigation of the response to anti-angiogenic therapy in breast cancer: New strategies for patient selection and opportunities for reconsidering anti-VEGF, anti-PI3K and checkpoint inhibition. EBioMedicine 2016;10:13-4.
Huang F, Cao J, Liu Q, Zou Y, Li H, Yin T, et al.
MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1β induced in human endometriosis stromal cells in vitro
. Int J Clin Exp Pathol 2013;6:2129-36.
Xia P, Xu XY. PI3K/Akt/mTOR signaling pathway in cancer stem cells: From basic research to clinical application. Am J Cancer Res 2015;5:1602-9.
Zheng L, Gong W, Liang P, Huang X, You N, Han KQ, et al.
Effects of AFP-activated PI3K/Akt signaling pathway on cell proliferation of liver cancer. Tumour Biol 2014;35:4095-9.
Li B, Wang Z, Zhong Y, Lan J, Li X, Lin H, et al.
CCR9-CCL25 interaction suppresses apoptosis of lung cancer cells by activating the PI3K/Akt pathway. Med Oncol 2015;32:66.
Yang S, Han H. Effect of cycloxygenase-2 silencing on the malignant biological behavior of MCF-7 breast cancer cells. Oncol Lett 2014;8:1628-34.
Wang L, Wang J, Xiong H, Wu F, Lan T, Zhang Y, et al.
Co-targeting hexokinase 2-mediated Warburg effect and ULK1-dependent autophagy suppresses tumor growth of PTEN- and TP53-deficiency-driven castration-resistant prostate cancer. EBioMedicine 2016;7:50-61.
Liu Y, Dong M, Yang Z, Pan S. Anti-diabetic effect of citrus pectin in diabetic rats and potential mechanism via PI3K/Akt signaling pathway. Int J Biol Macromol 2016;89:484-8.
Mendes Sdos S, Candi A, Vansteenbrugge M, Pignon MR, Bult H, Boudjeltia KZ, et al.
Microarray analyses of the effects of NF-kappaB or PI3K pathway inhibitors on the LPS-induced gene expression profile in RAW264.7 cells: Synergistic effects of rapamycin on LPS-induced MMP9-overexpression. Cell Signal 2009;21:1109-22.
Zhou J, Ling J, Song J, Wang Y, Feng B, Ping F, et al.
Interleukin 10 protects primary melanocyte by activation of stat-3 and PI3K/Akt/NF-κB signaling pathways. Cytokine 2016;83:275-81.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
[Table 1], [Table 2], [Table 3], [Table 4]