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ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 53  |  Page : 22-26

Optimization of microwave-assisted extraction of silymarin from Silybum marianum straws by response surface methodology and quantification by high-performance liquid chromatograph method


1 College of China Medicine, Zhejiang Pharmaceutical College, Ningbo, PR China
2 College of Pharmacy and Food Science, Tonghua Normal University, Tonghua, PR China

Correspondence Address:
Kun Teng
College of Pharmacy and Food Science, Tonghua Normal University 134000, Yucai Road No. 950 Tonghua
PR China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_556_16

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Background: Silybum marianum, a member of the Aster family, is a well-known Chinese herb and the source of a popular antioxidant that is extensively used in Asia. The abundant S. marianum straws are still underutilized and wastefully discarded to pollute the environment. Objective: To solve the above problem and better utilize S. marianum straws, the objective of this study was to optimize the conditions for extraction of silymarin from S. marianum straws. Materials and Methods: A combination of microwave-assisted extraction and response surface methodology (RSM) was used for silymarin from S. marianum straws and yield assessment by high-performance liquid chromatography method. The RSM was based on a five-level, four-variable central composite design (CCD). Results: The results indicated that the optimal conditions to obtain highest yields of silymarin were microwave power of 146 W, extraction time of 117 s, liquid-to-solid ratio of 16:1 mL/g, and ethanol concentration of 43% (v/v). Validation tests indicated that under the optimized conditions, the actual yield of silymarin was 6.83 ± 0.57 mg/g with relative standard deviation of 0.92% (n = 5), which was in good agreement with the predicted yield. Conclusions: The exploitation of the natural plant resources present very important impact for the economic development. The knowledge obtained from this work should be useful to further exploit and apply this material. Abbreviations used: MAE: Microwave-assisted extraction, RSM: Response surface methodology, HPLC: High-performance liquid chromatography, CCD: Central composite design, ANOVA: Analysis of variance.


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