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ORIGINAL ARTICLE
Year : 2017  |  Volume : 13  |  Issue : 51  |  Page : 392-400  

Bioactive constituents, radical scavenging, and antibacterial properties of the leaves and stem essential oils from Peperomia pellucida (L.) kunth


SAMRC Microbial Water Quality Monitoring Centre, Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Mail Bag X1314 Alice 5700, Eastern Cape, South Africa

Date of Submission14-Mar-2017
Date of Acceptance25-Apr-2017
Date of Web Publication11-Oct-2017

Correspondence Address:
Sunday O Okoh
Department of Biochemistry and Microbiology, SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Applied and Environmental Microbiology Research Group, Alice
South Africa
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_106_17

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   Abstract 


Background: Peperomia pellucida is an annual herbaceous ethnomedicinal plant used in the treatment of a variety of communicable and noncommunicable diseases in the Amazon region. Objective: The study aimed at profiling the bioactive constituents of the leaves and stem essential oils (LEO and SEO) of P. pellucida, their in vitro antibacterial and radical scavenging properties as probable lead constituents in the management of oxidative stress and infectious diseases. Materials and Methods: The EOs were obtained from the leaves and stem P. pellucida using modified Clevenger apparatus and characterized by a high-resolution gas chromatography-mass spectrometry, while the radicals scavenging and antibacterial effects on four oxidants and six reference bacteria strains were examined by spectrophotometric and agar diffusion techniques, respectively. Results: The EOs exhibited strong antibacterial activities against six bacteria (Escherichia coli [180], Enterobacter cloacae, Mycobacterium smegmatis, Listeria ivanovii, Staphylococcus aureus, Streptococcus uberis, and Vibrio paraheamolyticus) strains. The SEO antibacterial activities were not significantly different (P < 0.05) from the LEO against most of the test bacteria with minimum inhibitory concentration ranging between 0.15 and 0.20 mg/mL for both EOs. The two oils were bactericidal at 0.20 mg/mL against S. aureus while the minimum bactericidal concentration (0.15 mg/mL) of LEO against L. ivanovii was lower than of SEO (0.20 mg/mL) after 24 h. The LEO IC50value (1.67 mg/mL) revealed more radical scavenging activity than the SEO (2.83 mg/mL) and reference compounds against 2,2-diphenyl-1-picrylhydrazyl radical. The EOs also scavenged three other different radicals (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical , lipid peroxyl radical, and nitric oxide radical) in concentration-dependent manner. Conclusion: Our results suggest that apart from the indigenous uses of the plant extracts, the EO contains strong bioactive compounds with antibacterial and radicals scavenging properties and may be good alternative candidates in the search for novel potent antibiotics in this present era of increasing multidrug-resistant bacterial strains as well as effective antioxidants agents.
Abbreviations used: GC-MS: Gas chromatography-mass spectrometry, DPPH: 2,2-diphenyl-1-picrylhydrazyl, ABTS: 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, DMSO: Dimethyl sulfoxide, LP: Lipid peroxide radical, NO: Nitric oxide radical, LEO: Leaf essential oil, SEO: Stem essential oil, RC: Reference compound, TBARS: Thiobarbituric acid

Keywords: β-caryophyllene, antibacterial, limonene, radicals scavenging, Peperomia pellucida


How to cite this article:
Okoh SO, Iweriebor BC, Okoh OO, Okoh AI. Bioactive constituents, radical scavenging, and antibacterial properties of the leaves and stem essential oils from Peperomia pellucida (L.) kunth. Phcog Mag 2017;13, Suppl S3:392-400

How to cite this URL:
Okoh SO, Iweriebor BC, Okoh OO, Okoh AI. Bioactive constituents, radical scavenging, and antibacterial properties of the leaves and stem essential oils from Peperomia pellucida (L.) kunth. Phcog Mag [serial online] 2017 [cited 2017 Oct 23];13, Suppl S3:392-400. Available from: http://www.phcog.com/text.asp?2017/13/51/392/216344



Summary

  • Established gas chromatography-mass spectrometry technique was applied to quantitatively and qualitatively analyze the volatile constituents in Peperomia pellucida essential oil.(EO)
  • The Clinical and Laboratory Standards Institute (2014) guidelines were employed to evaluate the antibacterial effects of the EOs
  • Among the known prominent bioactive terpenoids, linalool 17.09%, limonene 14.25%, β-caryophyllene 12.52%, and linalyl acetate 10.15% were the main constituents of the EOs in this current study
  • The leaf and stem EOs were bactericidal at a concentration below 0.23 mg/mL against three multidrug-resistant bacteria and significantly scavenged known free radicals reported to be associated with contagious and oxidative stress-related disorders.



   Introduction Top


Infectious and noncommunicable diseases, particularly those due to multidrug-resistant microorganisms such as Staphylococcus, Escherichia coli, Enterococcus species, and reactive oxygen species, are almost impossible to combat.[1] The resistant rate of pathogens to vast synthetic antimicrobial agents coupled with rising side effects of antibiotics deserves novel therapies for efficient public health care.[2] Accordingly, some articles on bioactive phytochemical including alkaloids, polyphenol, flavonoids, and essential oil (EO) constituents have been suggested in recent years as possible option.[3],[4],[5] Evidence from the previous studies suggest that EO has therapeutic properties and could stand as alternative of antibiotics against certain pathogenic bacteria species, besides filamentous fungi and yeasts.[5],[6] Some plants' volatile oils (EOs) have been shown to speedily diffuse cell membrane of bacteria owing to their permeability properties across biological lipid barriers.[4],[6] This interaction can lead to membrane instability and consequently leakage of the bacterial important intracellular components and ultimate cell death. Cell wall, cell membrane, intracellular proteins, nucleic acids or enzymes, and few others are vital target sites for drug design, and some volatile oil compounds have these specialized sites of the cell as their target.[7],[8],[9]

Enzymatic antiradical defense systems made up of glutathione peroxidase, catalase, superoxide dismutase, as well as other endogenous antiradical molecules, especially glutathione, do scavenge oxygen-derived-free radicals produced in physiological and pathological processes.[10] However, the scavenging of oxidants generated including superoxide radical, lipid peroxyl radical (LP ), nitric oxide radical (NO ), and hydroxyl (HO•) produced during metabolic activities and environmentally induced radicals overwhelms the naturally produced antiradicals.[11] Man has used spices, fruits, vegetables, and plant's decoction now acknowledged containing potent secondary metabolites against diseases for more than 20 decades. In recent time, some studies have shown secondary metabolites including phenols, flavonoids, and alkaloids from plants and their EOs are potent antiradicals.[12],[13],[14] EO could function as a credible option to synthetic antibiotics due to its property to penetrate the cell membrane as well as radical scavenger.[15] The European Commission has approved EO constituents including limonene, linalool, menthol, and caryophyllene that possess such properties as food flavors and additives in cosmetics products.[9],[15],[16]

Peperomia pellucida (shiny bush, silver bush) of the family Piperaceae is an annual herbaceous plant. It grows in rainy (often in the spring) season to height of 15–46 cm in humid loose soil, especially under the trees. It is commonly found in West African rainforest belt including Southeast and Southwest Nigeria and many tropical Asian and South American countries.[17],[18] Ethnomedical reports of P. pellucida shows that the leaf uses vary depending on the region where it is found. In the Amazon region, it has been used to reduce cholesterol level, as a diuretic, dementia disorder, and in treating cardiac arrhythmia.[18],[19] In Ayurvedic records, the leaves and stem aqueous mixture is used in treating hemorrhage, fever, headache, abdominal pain, wounds dressing and as cough suppressant.[17],[20] The decoction of the whole plant in India served as potent medication in rheumatism, renal disorders, breast cancer, boils, and small pox.[18],[21],[22] Previous pharmacological studies revealed that the solvents' crude extracts exhibit significant analgesics, antimicrobial, anti-inflammatory, and anti-protozoa activities and cytotoxic to breast cancer cell lines.[19] Another study by Xu et al.[23] of the solvents leaves crude extracts indicated alkaloids, sterols, flavonoids, and styrene as dominant bioactive compounds of P. pellucida. Previous investigation of the leaf essential oil (LEO) revealed apiole and β-caryophyllene as the major volatile compounds.[24] Nevertheless, information on radical scavenging effects on a variety of free radicals and antibacterial activity on multidrug-resistant bacteria strains is scanty, while comparative studies on the LEO and stem EO (SEO) constituents of P. pellucida are lacking. This information is imperative for thorough understanding of the plant bioactive value and economic evaluation. We aimed in this present study to characterize the bioactive constituents and to evaluate the radical scavenging and antibacterial effects of the LEO and SEO of P. pellucida.


   Materials and Methods Top


Analytical reagents

The chemicals and reagents used included the following: Mueller-Hinton agar from Oxford Ltd (Hampshire, England), dimethyl sulfoxide (DMSO) from Fluka Chemicals (Buchs, Switzerland). 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) from Sigma-Aldrich (St. Louis, USA). All chemicals and reagents were of analytical grade.

Plant material

P. pellucida was collected in August 2016 from Southwest Nigeria at the Forest Research Institute of Nigeria, Ibadan. A plant taxonomist authenticated the plant, and the sample was kept in the Lagos University Herbarium (LUH) with voucher specimen number LUH 6956. The leaves were left to air-dry at an ambient room temperature for 5 days, while the stem was cut into smaller pieces and air-dried for 7 days. They were pulverized and the EO extracted for 3 h from each (200 g) using modified Clevenger-type apparatus as previously described.[25] The hydrodistillation experiment was carried out thrice on the leaf and stem separately to obtain enough oil for bioactivity assays. The two EOs were dried with anhydrous sodium sulfate and stored in tinted vials at 4°C. The EO yield was then computed per gram (w/w%) of the plant sample.

Characterization of the essential oils

We utilized a gas chromatography/mass spectrometry (GC/MS) to analyze and identify the EO constituents. The analysis was carried out on Agilent 5977A mass spectra data (MSD) and 7890 GC system, Chemetrix (Pty) Ltd, Agilent Technologies, DE (Germany), with a Zebron-5MS (ZBMS 30 m × 0.25 mm × 0.25 um) (5% - phenyl methyl polysiloxane). The temperature and column conditions were applied: the injector, source, and oven temperature set at 280°C, 280°C, and 70°C, respectively. GC grade helium at a flow rate of 2 mL/min and splitless 1 mL injection was used. The ramp settings were 15°C/min to 120°C, then 10°C/min to 180°C, then 20°C/min to 270°C, and held to for 3 min. Subsequently, identification of each constituent was ascertained using agreement of their MSD with the reference held in the computer library (Wiley 275, New York). Furthermore, matching the retention index of each compound with those in literature was also employed in identifying the compounds. The peak areas were used to obtain total percentage composition of oil.

Antibacterial activity

Bacteria suspensions test

Four multidrug-resistant reference bacterial strains and two bacteria isolates from our laboratory stock culture confirmed to be multidrug-resistant bacteria [26],[27] were used for the antibacterial test. The reference and laboratory bacterial strains consist of four Gram-positive bacteria: Staphylococcus aureus (NCINB 50080), Listeria ivanovii (ATCC19119), Mycobacterium smegmatis (ATCC 19420), Streptococcus uberis (ATCC 29213) and three Gram-negative bacteria: Enterobacter cloacae ( ATCC 13047), E. coli 180, and Vibrio paraheamolyticus. All the test strains were confirmed to be resistant to ampicillin, cefuroxime, tetracycline, nalidixic, cephalexin, sulfamethoxazole, and streptomycin [27] were tested against the oils and ciprofloxacin following Clinical and Laboratory Standards Institute (2014) guidelines. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) potentials of the EOs and controls were determined.

Minimum inhibitory concentration and minimum bactericidal concentration evaluation

The microdilution technique was used to evaluate the minimum inhibitory concentrations (MICs). Eight hundred, 900, 950, 975, and 987.5 μL of Mueller-Hinton Broth (MHB) were added into each Eppendorf tube. Five hundred milligrams of both SEO and LEO stocks after evaporation of n-hexane was separately dissolved in DMSO (500 μL) and each solution vortexed. Thereafter, aliquots of 200, 100, 50, 25, and 12.5 μL were added, respectively, into each tube containing MHB to bring the final volume in each to 1 mL, and the mixtures were properly vortexed. The inoculum suspension (20 μL) of each test bacterial isolate (0.5 McFarland, ~1 × 108 CFU/mL) was added subsequently, vortexed to permit adequate mixing of the EO and broth. Ciprofloxacin and DMSO served as the positive and negative controls, respectively. The tubes were then subjected to incubation for 24 h at 37°C. The lowest concentration without visible growth was reported as the MIC. MBC was examined by pour plate method of all tube content without visible growth in the MIC technique above onto fresh nutrient agar plates; thereafter, plates were incubated at 37°C for 24 h. The lowest amount of concentration of EO that does not yield any culture growth on the solid medium at the end of incubation period was recorded as MBC.[28] The experiment was carried out in parallel triplicate and average value was recorded.

Antiradical assays

DPPH , ABTS , NO , and LP inhibiting tests were performed to determine the antiradical effects of the two EOs.

2,2-diphenyl-1-picrylhydrazyl assay

The DPPH test was performed as described by Liyana-Pathirana and Shahidi [29] with a slight modification (DMSO used instead of methanol). Concisely, a solution of DPPH (2.7 mM) in DMSO was prepared; afterward, 1 mL of it was added to the EO (1 mL) dissolved in DMSO which holds double-fold concentration (0.025–0.50 mg/mL) of the EO as well as the reference standard (RC). All mixtures were then vortexed and reacting solutions were then incubated in a dark chamber at ambient temperature for 30 min. Thereafter, absorbance of the reaction solution was read against a reference blank containing DMSO at 517 nm. EO's potency to reduce DPPH to neutral molecule was computed as radical scavenging percentage using the following formula:

% radical scavenging of DPPH by EO or RC = {(Absccontrol − Abscsample)}/(Absccontrol) × 100 (a)

Where Absccontrol is the absorbance of the DPPH radical + DMSO and Abscsample is the absorbance of DPPH radicals + essential oil/RC. The assay was carried out in parallel triplicate.

The IC50 is that concentration of the EO or reference compound (RC) (positive control) required reducing 50% of the DPPH radicals. This precise value was obtained from the regression equation generated from standard curve produced with increasing concentrations against inhibitions and results compared to that of RC.

2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assay

We used the cation radicals scavenging (ABTS •+) procedure of Re et al.[30] with some modification according to Witayapan et al.[31] by mixing 1:1 volumes of ABTS 7.00 mM and 4.90 mM of K2S2O8 solution. The mixed solution was kept in a dark chamber at ambient temperature for 12 h. Thereafter, the generated ABTS •+ was equilibrated at 734 nm with DMSO to its absorbance (0.705 ± 0.001). To carry out the assay, 1 mL of 0.025–0.50 mg/mL solutions of the test samples (SEO and LEO) in DMSO was mixed with 1 mL ABT •+ solution, bringing final volume of each mixture to 2 mL. The reaction solution was kept in dark chamber for 7 min. Subsequently, its absorbance was measured spectrophotometrically at 760 nm. The EO as well as RC radical scavenging effects on ABTS •+ was expressed in terms of percentage (%) inhibition of ABTS •+ using equation (a) described in DPPH radical test. The test was performed in parallel triplicates and average value calculated.

Thiobarbituric acid assay

The EOs and RCs radical scavenging effects on LP were measured using the technique as presented by Badmus et al.[32] utilizing egg yolk as lipid-rich source. The test samples (0.1 mL) at increasing concentration (0.025, 0.05, 0.10, 0.20, and 0.5 mg/mL in DMSO) were added to 10% egg yolk homogenate (0.5 mL) and the reaction mixture made up to 1 mL. Thereafter, 50 μL of 0.07 M iron (11) sulfate heptahydrate was added to induce lipid peroxidation and the solution incubated for 30 min at ambient temperature. Thereafter, 1.5 mL of acetic acid (10%) (pH 3.50) and 1.5 mL of 0.08% 2-thiobarbituric acid, plus 20% trichloroacetic acid, and 1.1% sodium dodecyl sulfate were added and the mixture was heated at 65°C for 1 h. Upon cooling, the solution was vortexed and n-butanol (0.5 mL) was added to the solution. The solution was then centrifuged at 3000 rpm for 10 min. The resultant upper layer was then aspirated and its absorbance at 532 nm read. The equation (a) described in DPPH radical test was thereafter used to calculate the scavenging effects (%) of the EO and RC on the LP generated. The test was carried out in parallel triplicate and average calculated.

Measurement of the inhibition of nitric oxide radicals assay

The radical scavenging effect of the EO on NO was investigated according to the modified method described by Makhija et al.[33] Sodium nitroprusside molecule in aqueous solution at physiological pH (7.2) decomposed to produce NO radicals. In aerobic conditions, the radical reacts with oxygen molecule producing nitrite and nitrate as stable molecules and applying Griess reagent these resultant molecules are measured.[34] To 1.0 mL of the EO at increasing doses (0.025, 0.05, 0.10, 0.20, and 0.5 mg/mL prepared in DMSO) was added to 1.0 mL (10 mM) of sodium nitroprusside solution. The solutions were incubated for 110 min at ambient temperature. Thereafter, 1 mL of the aliquot was added to Griess solution (1%, sulfanilamide, 1% N-naphthyl-ethylenediamine hydrochloride in 2% orthophosphoric acid). Subsequently, absorbance of the color developed was then measured spectrophotometrically at 546 nm against the reagent blank. The scavenging effect (%) was then obtained using equation (a) described in DPPH radical test. The assay was carried out in parallel triplicates and mean value calculated.

Statistical analysis

All experiments involving quantitative test were performed in parallel triplicate (n = 3). All results expressed as means ± standard deviation. Percentage scavenging of radical was concentration-dependent and regression equation generated from the standard curve for each radical scavenger was used to calculate its IC50 value. t-test correlation analysis was employed to test significant differences between the concentrations versus percentage of radical scavenging effect, carried out using SPSS 15.0 for windows (IBM SPSS Inc., OLRAC SPS registration number 2012/1786646/07). At P < 0.05 confidence level, result was considered being significantly different.


   Results Top


Constituents of the essential oils

The yields, constituents of the EOs extracted, molecular formula as well as of methods of identification each constituent from the leaves and stem oils of P. pellucida, are presented in [Table 1]. The LEO yield of 0.51% was significantly (P < 0.05) higher than the SEO 0.32%, while the color and aroma of the two oils were similar. Compared to the yields (0.05%–0.58%) of EOs extracted by hydrodistillation from China and elsewhere,[13],[35] the LEO of P. pellucida plant could be considered as rich in EO. The identified bioactive constituents of the plant's EO predominantly monoterpenoid alcohols, sesquiterpenes, aromatic and aliphatic aldehydes [Figure 1] might be responsible for bioactivity of the EOs. Eighteen constituents were found in the SEO with one unidentified constituents, while the LEO contained 16 constituents representing 86.02% and 80.36% of the total oil content, respectively [Table 1]. In the SEO, monoterpenoids and monoterpenes content accounted for 60.27%, followed by sesquiterpenoids 11.71%, while the diterpenoid content was phytol 3.41%. Among the dominant monoterpenoids constituents, linalool (12.60%) was the highest, followed by d-limonene (10.7l%) and α-terpineol (10.57%), while β-caryophyllene (11.47%) was major sesquiterpene. In the LEO, d-limonene (14.25%) and β-caryophyllene (12.52%) were the major constituents. In addition to similar monoterpenoids (35.04%) identified in the SEO, the quantity of aldehydes (18.90%) constituents found in the LEO was higher than those in the SEO. The chemical profiles of the two oils significantly differed; there were traces of borneol and phytol in LEO, while 9-octadecenoic acid and terpen-4-ol were not among the constituents LEO. Conversely, these components were present in significant amount in the SEO of P. pellucida. The percentage content of caryophyllene and limonene was higher in LEO than in the SEO. Among the previously reported bioactive compounds in this study are borneol, (+)-4-carene, camphene, β-caryophyllene, 9-octadecenoic acid, phytol, and pinene.[19],[34],[36] In this present study, more constituents (16) were found in EO compared to those from P. pellucida leafmethanol extract in Wei et al.'s [19] study. However, some of the constituents reported by Rajendra et al.[37] in EO of P. pellucida from India including carotol, apiol, and camphor were not among those we identified. The discrepancies observed in P. pellucida EOconstituents grown in Nigeria, China, and elsewhere may be due to climatic, seasonal, and environmental variation. Other factors including the age of the plant, humidity of the harvested plant material, and the existence of chemotype, may also influenced EO's constituents.[13],[38] To the best of our knowledge, this is the first report of comparative investigation of the bioactive constituents and bioactive properties in the LEO and SEO of P. pellucida and HMOS was reported in Kumaradevan et al.[39] and Parmar et al.[40] studies as a strong antimicrobial phytochemical compound.
Table 1: Essential oils constituents of Peperomia pellucida

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Figure 1: Structures of some major bioactive constituents in Peperomia pellucida essential oil

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Antibacterial activity of essential oils

The leaves and stem oils of P. pellucida effectively exhibited inhibitory activity against the three reference strains bacteria (S. aureus, E. cloacae, and L. ivanovii, as well as two isolates – E. coli 180 and V. paraheamolyticus) confirmed to be multidrug-resistant bacteria from our laboratory stock culture.[26] The SEO antibacterial activities were not significantly different from the LEOagainst most of the test bacteria with MIC ranging between 0.15 and 0.20 mg/mL for EOs. The LEO and SEO were bactericidal at 0.15 and 0.20 mg/mL against L. ivanovii, respectively, while the MBCs against S. aureus for both EOs were similar (0.20 mg/mL) after 24 h. The LEO also exhibited bactericidal effect against E. coli 180 at 0.20 mg/mL [Table 2]. However, the two oils displayed more bacteriostatic activity at higher dose (>0.20 mg/mL) against the Gram-negative test bacteria (V. paraheamolyticus and E. cloacae)except E. coli 180 and exhibited lower antibacterial activity than the positive control drug (ciprofloxacin). The differences in antibacterial activity may be linked to net repulsion of the complex outer membrane in Gram-negative bacteria. This has been shown in the previous studies to contain hydrophilic lipopolysaccharide (a two-lipid bilayer).[8],[41] Consequently, higher tolerance is created toward hydrophobic antibacterial terpene molecules common in EOs.[8] The activity of the stem and leaves oils of P. pellucida against the bacteria also differed; the variation observed in the constituent's profiles of two oils [Table 1] may account for their varied bioactivity in this present study.
Table 2: Antibacterial activities of the essential oils Peperomia pellucida

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Essential oils radical scavenging activities

The radical scavenging activities of the P. pellucida EOs (LEO and SEO) were studied in vitro in four different oxidants (DPPH , ABTS •+, LP , and NO ) models. The scavenging effect of both EOs and RCs (Vitamin C and β-carotene) on the test radicals were concentration dependent (0.025–0.5 mg/mL). The radical scavenging effects of LEO on DPPH at increasing doses (0.025, 0.05, 0.10, 0.2, and 0.5 mg/mL) were significantly different (P < 0.05) than the SEO as well as the two RCs (++). The SEO and Vitamin C demonstrated comparable effect (ss) at low (0.025–0.10 mg/mL), while at high doses (0.2 and 0.5 mg/mL), scavenging effects of SEO on DPPH were better (++) than Vitamin C [Figure 2]. The DPPH scavenging protocol is based on the premise that a substance donating an atom of hydrogen or an electron is an antioxidant or radical scavenger and its property is demonstrated as DPPH color changes (purple to yellow) in the test sample due to formation of neutral DPPH-H molecule upon receiving H atom from an antiradical.[42] However, DPPH model is not a specific radical species test but general radicals scavenging potency of an antioxidant.[43] Therefore, to evaluate the precise antiradical efficacy of LEO and SEO of P. pellucida, we quantitatively investigated the presumed radical scavenging effects using different specific radical (LP and NO ) and a cation radical (ABTS •+).
Figure 2: Radical scavenging effects of Peperomia pellucida essential oil and reference compounds on 2,2-diphenyl-1-picrylhydrazyl radicals

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In the four-radical scavenging in vitro assays, the LEO and SEO of P. pellucida showed effective radicals scavenging potencies against the different radicals, indicating that they are good electron or H atom donors to DPPH, ABTS radicals, and exhibited valuable scavenging property against lipid and NO radicals [Figure 1],[Figure 2],[Figure 3],[Figure 4]. Assessing the IC50 values from regression equations generated from standard curves as well as t-test analysis for significant difference of % scavenging effects versus concentrations, both oils reduced the DPPH to a neutral DPPH-H molecule attaining 50% decrease with IC50 value of 1.67 ± 0.01 mg/mL for LEO and 2.82 ± 0.11 mg/mL for the SEO. The RCs radical scavenging effects on DPPH (Vitamin C 2.86 ± 0.01 and β-carotene 2.02 ± 0.12 mg/mL) values were significantly lower than LEO (P < 0.05) [Table 3].
Figure 3: Radical scavenging effects of Peperomia pellucida essential oil and reference compounds on 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radicals

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Figure 4: Antiradical effects of Peperomia pellucida extracts and reference compounds on lipid peroxyl radicalswith

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Table 3: Radical scavenging capacity of essential oils extracted from Peperomia pellucida IC50 (mg/mL)

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The scavenging effects on the ABTS radicals by the SEO and Vitamin C were lower compared to results obtained in DPPH assay. However, LEO and β-carotene exhibited high effects especially at low concentrations [Figure 3]. The LEO IC50 value of 1.94 ± 0.11 mg/mL further confirmed its higher radical scavenging strength over the SEO (2.34 mg/mL) and Vitamin C (2.70 mg/mL) indicated in DPPH model. However, unlike in the DPPH assay, the radical scavenger completely decolorized the blue color of the oxidant (ABTS •+) solution, turning into neutral molecules (colorless form) from the lowest to highest concentrations (0.025–0.50 mg/mL). The difference observed in activities of SEO and LEO against the two different oxidants (DPPH and ABTS •+) could be attributed to many factors including the complexity, polarity and isomers selectivity of the radicals. In addition, the ease at which the oils solvate the radical's medium may differ and these variables have been suggested to influence potency of volatile constituents in scavenging species of radicals.[43]

The LP scavenging effects of P. pellucida of the two EOs and the RC were concentration-dependent [Figure 4] as in DPPH and ABTS assays. Remarkably, at low concentrations (0.05–0.025 mg/mL), scavenging effects of LEO were above 40% and higher than the RC. However, as the concentration increases (0.2–0.5 mg/mL), SEO exhibited moderate scavenging effects of on LP , while β-carotene and LEO demonstrated higher effects than SEO and Vitamin C. Interestingly, the assessed IC50 values from the regression equation generated from each standard curve, indicated a higher scavenging strength (1.61 ± 0.02 mg/mL) for LEO than the SEO (1.88 mg/mL) as well as the RC. Notable in the lipid peroxidation model is the significant difference between the radical scavenging capacity of EOs and the Vitamin C (2.9 ± 0.00 mg/mL) [Table 3]. This may be ascribed to the bioactive constituents [Figure 1], predominantly aliphatic and aromatic alcohols that might have donated H atoms to H2O2, thus reducing it to 2H2O.

In the NO test, the LEO was significantly more (++) effective in scavenging NO than the SEO and RC at different doses (0.50, 0.20, 0.10, and 0.025 mg/mL) [Figure 5]. Unlike in ABTS at low doses (0.05 and 0.025 mg/mL), the two EOs and RCs demonstrated higher radical scavenging effects. The effects of LEO and SEO were significantly different (++) and superior to RC at 0.05 mg/mL. However, as the concentrations increase to 0.2 mg/mL, scavenging effect differences between the LEO, SEO, and RC were significant (++) with LEO having the highest, followed by β-carotene, then SEO, while Vitamin C had the least effect in scavenging NO generated [Figure 5]. The LEO IC50 value of 2.10 ± 0.11 mg/mL indicated that it has higher radical scavenging strength over β-carotene (2.39 mg/mL) and Vitamin C, while the IC50 for SEO (2.40 mg/mL) and β-carotene does not differ significantly (SS) P < 0.05 [Table 3].
Figure 5: Radical scavenging effects of Peperomia pellucida essential oil and reference compounds on nitric oxide radicals

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   Discussion Top


In recent years, few studies on some of the EO constituents we found in the LEO and SEO of P. pellucida have reported that some of them are potent bioactive secondary metabolites. For example, limonene,[16] camphene,[44] α-pinene,[45] borneol,[46] and linalool [47] are known to be strong bioactive compounds.[48] Furthermore, the presence of phytol in the LEO and SEO might have enhanced the bioactivity. Phytol, a bioactive diterpenoid alcohol, is often used as a precursor to produce synthetic forms of Vitamin E and Vitamin K1. Santos et al.reported phytolto demonstrate good antioxidant effect in vivo as well as its high capacity to scavenge HO , NO and prevent the formation of LP radicals.[49]In addition to phytol, other bioactive terpenoids, including linalyl acetate (10.15%), citronellol (3.40%), phenyl ethyl alcohol (3.18%), and phenylpropanoic acid (3.15%), found in the LEO and SEO might have enhanced the bioactivity of both EOs in this study suggesting synergetic or additive interaction of these constituents in LEO and SEO, especially in scavenging radicals and inhibitory effects on test bacteria.[50],[51] Furthermore, the dominant constituent (linalool 12.60%–17.09%) identified in the SEO and LEO could have reacted with DPPH , ABTS , LP , and NO radicals through various mechanisms suggested by Foti and Amorati.[52] The result in this current study agrees with other reports that have implicated aliphatic terpene with radical scavenging properties, while effect of sesquiterpene (C15), for example, β-caryophyllene (11.47%–12.52%), found in SEO and LEO, is similar to the property of phenolic compounds or alpha tocopherol.[7],[13],[15],[53] The potential to scavenge different radicals and exhibit inhibitory activity against four referencebacterial strains and two bacteria isolates from our laboratory stock culture confirmed to be multidrug-resistant bacterial strains as observed in this current study is quite remarkable. This observation may suggest that LEO of P. pellucida could possibly be a new potential candidate for managing infectious diseases as well as oxidative stress-related disorders such as cancers, diabetic nephropathy, Alzheimer's disease, and arteriosclerosis.[53],


   Conclusion Top


This present study indicates that apart from the traditional uses of P. pellucida, the LEO and SEO contained strong bioactive constituents; thus, they could be good candidates as new antimicrobial agents in this present era of increasing multidrug-resistant bacterial strains, also an option to synthetic antioxidant and may be used as food preservatives.

Acknowledgement

We are grateful to the South Africa Medical Research Council, GMRDC, and the University of Fort Hare for financial support.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Dickson RA, Houghton PJ, Hylands PJ, Gibbons S. Antimicrobial, resistance-modifying effects, antioxidant and free radical scavenging activities of Mezoneuron benthamianum baill. Securinega virosa roxb. & Wlld. and Microglossa pyrifolia lam. Phytother Res 2006;20:41-5.  Back to cited text no. 1
    
2.
Aly M, Balkhy HH. The prevalence of antimicrobial resistance in clinical isolates from gulf corporation council countries. Antimicrob Resist Infect Control 2012;1:26.  Back to cited text no. 2
    
3.
Van VS, Kamatou GP, Viljoen AM. Volatile composition and antimicrobial activity of twenty commercial frankincense essential oil samples. S Af J Bot 2010;76:686-91.  Back to cited text no. 3
    
4.
Prakash D, Gupta C, Sharma G. Importance of phytochemicals in nutraceuticals. J Chin Med Res Dev 2011;1:70-8.  Back to cited text no. 4
    
5.
Valente J, Zuzarte M, Gonçalves MJ, Lopes MC, Cavaleiro C, Salgueiro L, et al. Antifungal, antioxidant and anti-inflammatory activities of Oenanthe crocata L. essential oil. Food Chem Toxicol 2013;62:349-54.  Back to cited text no. 5
    
6.
Rammanee K, Hongpattarakere T. Effects of tropical citrus essential oils on growth, afflatoxin production and ultrastructure alterations of Aspergillus flavus and Aspergillus parasiticus. Food Bioproc Tech 2011;4:1050-9.  Back to cited text no. 6
    
7.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils – A review. Food Chem Toxicol 2008;46:446-75.  Back to cited text no. 7
    
8.
Helander IM, Akakomi H, Latva-Kala K, Mattila-Sandholm T, Pol I, Eddy J. Characterization of the action of selected essential oil components on gram-negative bacteria. J Agric Food Chem 1998;46:3590-5.  Back to cited text no. 8
    
9.
Hyldgaard M, Mygind T, Meyer RL. Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Front Microbiol 2012;3:12.  Back to cited text no. 9
    
10.
Murray AP, Rodriguez S, Frontera MA, Tomas MA, Mulet MC. Antioxidant metabolites from Limonium brasiliense (Boiss.) Kuntze. Z Naturforsch C 2004;59:477-80.  Back to cited text no. 10
    
11.
Nagendrappa CG. An appreciation of free radical chemistry-3, free radicals in diseases and health. Resona 2005;10:65-73.  Back to cited text no. 11
    
12.
Saikat S, Chakraborty R, Sridhar CY, Reddy SR, Biplab D. Free radicals, antioxidants diseases and phytomedicine: Current status and future prospect. Int J Pharm Sci Rev Res 2010;3:91-100.  Back to cited text no. 12
    
13.
Wang W, Wu N, Zu YG, Fu YJ. Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. Food Chem 2008;108:1019-22.  Back to cited text no. 13
    
14.
Nweze EI, Okafor JI. Activities of a wide range of medicinal plants and Essential oil vs. Scedospaorium isolates. Am Eur J Res 2010;5:161-9.  Back to cited text no. 14
    
15.
Kotamballi N, Chidambara M, Jayaprakasha GK, Bhimanagouda SP. Antiinflammatory mediated applications of monoterpenes found in fruits. Am Chem Soc 2013;10:121-31.  Back to cited text no. 15
    
16.
Penoel MD, Penoel R. Natural Health Care Using Essential Oils. Abundant Health. New Zealand; 2012. Available from: http://www.abundanthealth4u.com.  Back to cited text no. 16
    
17.
Habsah M, Yosie A, Baker K, Siang CC, Syamsumir DF, Alias A, et al. Effect of drying method on antimicrobial, antioxidant and isolation of bioactive from P. pellucida. J Chem Pharm Res 2015;7:578-84.  Back to cited text no. 17
    
18.
Oloyede KG, Onocha AP, Olaniran BB. Phytochemical, toxicity, antimicrobial and antioxidant screening of leaf extracts of P. pellucida. Adv Environ Biol 2011;5:3700-9.  Back to cited text no. 18
    
19.
Wei LS, Wee W, Siong JY, Syamsumir DF. Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract. Acta Med Iran 2011;49:670-4.  Back to cited text no. 19
    
20.
Aziba PI, Adedeji A, Ekor M, Adeyemi O. Analgesic activity of Peperomia pellucida aerial parts in mice. Fitoterapia 2001;72:57-8.  Back to cited text no. 20
    
21.
Khan A, Rahman M, Islam MS. Neuropharmacological effects of Peperomia pellucida leaves in mice. DARU 2008;16:35-40.  Back to cited text no. 21
    
22.
de Fátima Arrigoni-Blank M, Dmitrieva EG, Franzotti EM, Antoniolli AR, Andrade MR, Marchioro M, et al. Anti-inflammatory and analgesic activity of Peperomia pellucida (L.) HBK (Piperaceae). J Ethnopharmacol 2004;91:215-8.  Back to cited text no. 22
    
23.
Xu S, Li N, Ning MM, Zhou CH, Yang QR, Wang MW, et al. Bioactive compounds from Peperomia pellucida. J Nat Prod 2006;69:247-50.  Back to cited text no. 23
    
24.
Helio LS, Maria GB, Eloisa HA. Andrade, JGS. The essential oils of Peperomia pellucida Kunth and P. circinnata Link. Flavour Fragr J 1999;14:312-4.  Back to cited text no. 24
    
25.
Okoh SO, Asekun OT, Familoni OB, Afolayan AJ. Composition and antioxidant activities of leaf and root volatile oils of Morinda lucida. Nat Prod Commun 2011;6:1537-41.  Back to cited text no. 25
    
26.
Iweriebor BC, Iwu CJ, Obi LC, Nwodo UU, Okoh AI. Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern cape of South Africa. BMC Microbiol 2015;15:213.  Back to cited text no. 26
    
27.
Iwu CJ, Iweriebor BC, Obi LC, Basson AK, Okoh AI. Multidrug-resistant Salmonella isolates from swine in the Eastern Cape Province, South Africa. J Food Prot 2016;79:1234-9.  Back to cited text no. 27
    
28.
Ramalivhana JN, Iweriebor BC, Obi CL, Samie A, Uaboi-Egbenni P, Idiaghe JE, et al. Antimicrobial activity of honey and medicinal plant extracts against Gram negative microorganisms. Afr J Biotechnol 2014;13:616-25.  Back to cited text no. 28
    
29.
Liyana-Pathirana CM, Shahidi F. Antioxidant properties of the essential oils from lemon, grape, coriander, clove and their mixtures. J Sci Food Agric 2006;86:477.  Back to cited text no. 29
    
30.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231-7.  Back to cited text no. 30
    
31.
Witayapan N, Sombat C, Siriporn O. Antioxidant and antimicrobial activities of Hyptis suaveolens essential oil. Sci Pharm 2007;75:35-46.  Back to cited text no. 31
    
32.
Badmus JA, Odunola OA, Obuotor E. Phytochemical and in vitro antioxidant potentials of Holarrhena floribunda leaf. Afr J Biotechnol 2010;9:340-6.  Back to cited text no. 32
    
33.
Makhija IK, Aswatha RH, Shreedhara CS, Vijay KS, Devkar R. In-vitro antioxidant studies of Sitopaladi churna, a polyherbal ayurvedic formulation. Free Radic Antioxid 2011;1:37-41.  Back to cited text no. 33
    
34.
Giustarini D, Rossi R, Milzani A, Dalle-Donne I. Nitrite and nitrate measurement by Griess reagent in human plasma: Evaluation of interferences and standardization. Methods Enzymol 2008;440:361-9.  Back to cited text no. 34
    
35.
Okoh OO, Afolayan AJ. The effects of hydrodistillation and solvent free microwave extraction methods on the chemical composition and toxicity of essential oils from the leaves of Mentha longifolia L. Afr J Pharm Pharm 2011;5:2474-8.  Back to cited text no. 35
    
36.
Potzernheim MC, Bizzo HR, Silva JP, Vieira RF. Chemical characterization of essential oil constituents of four populations of P. aduncum L. from Distrito Federal, Brazil. Biochem Syst Ecol 2012;42:25-31.  Back to cited text no. 36
    
37.
Rajendra CP, Prakash G, Amit C. Essential oil composition of Peperomia pellucida (L.) Kunth from India. J Essent Oil Res 2015;27:89-95.  Back to cited text no. 37
    
38.
Valero D. The addition of essential oils to MAP as a tool to maintain the overall quality of fruits. Trends Food Sci Tech 2008;19:464-71.  Back to cited text no. 38
    
39.
Perussi JR. Photodynamic inactivation of microorganisms. Quim Nova 2007;30:988-94.  Back to cited text no. 39
    
40.
Guerrini A, Sacchetti G, Rossi D, Paganetto G, Muzzoli M, Andreotti E, et al. Bioactivities of Piper aduncum L. and Piper obliquum ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador. Environ Toxicol Pharmacol 2009;27:39-48.  Back to cited text no. 40
    
41.
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.  Back to cited text no. 41
    
42.
Vallianou I, Peroulis N, Pantazis P, Hadzopoulou-Cladaras M. Camphene, a plant-derived monoterpene, reduces plasma cholesterol and triglycerides in hyperlipidemic rats independently of HMG-CoA reductase activity. PLoS One 2011;6:e20516.  Back to cited text no. 42
    
43.
Elanur A, Hasan T, Fatime G. Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells. Biologia 2013;68:1004-9.  Back to cited text no. 43
    
44.
Horvathova E, Katarina K, Srancikova A, Hunakova L, Galova E, Sevcovicova A, Slamenova D. Borneol administration protects primary rat hepatocytes against exogenous oxidative DNA damage. Oxf J Life Sci 2012;27:581-8.  Back to cited text no. 44
    
45.
Ganesan P, Phaiphan A, Mourugan Y, Baharin B. Comparative study of bioactive compounds in curry and coriander leaves – An update. J Chem Pharm Res 2013;5:590-4.  Back to cited text no. 45
    
46.
Duke JA. Phytochemical and Ethno-Botanical Databases. Florida USA: CRS Press, Inc.; 2008.  Back to cited text no. 46
    
47.
Santos CC, Salvadori MS, Mota VG, Costa LM, de Almeida AA, de Oliveira GA, et al. Antinociceptive and antioxidant activities of phytol in vivo and in vitro models. Neurosci J 2013;2013:949452.  Back to cited text no. 47
    
48.
Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005;49:2474-8.  Back to cited text no. 48
    
49.
Probst IS, Sforcin JM, Rall VL, Fernandes AA, Fernandes A. Antimicrobial activity of propolis and essential oils and synergism between these natural products. J Vernom Anim Toxins Incl Trop Dis 2011;17:157-69.  Back to cited text no. 49
    
50.
Naito Y, Uchiyama K, Yoshikawa T. Oxidative stress involvement in diabetic nephropathy and its prevention by astaxanthin. Oxid Stress Disord 2006;21:235-42.  Back to cited text no. 50
    
51.
Foti MC, Amorati R. Non-phenolic radical-trapping antioxidants. J Pharm Pharmacol 2009;61:1435-48.  Back to cited text no. 51
    
52.
Paz-Elizur T, Sevilya Z, Leitner-Dagan Y, Elinger D, Roisman LC, Livneh Z. DNA repair of oxidative DNA damage in human carcinogenesis: Potential application for cancer risk assessment and prevention. Cancer Lett 2008;266:60-2.  Back to cited text no. 52
    
53.
Fini MA, Johnson RJ, Stenmark KR, Wright RM. Hypertension, nitrite, xanthine oxidoreductase catalyzed nitric oxide generation: Pros and cons. Hypertension 62, e9. floribunda leaf. Afr J Biotech 2013;9:340-6.  Back to cited text no. 53
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

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