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ORIGINAL ARTICLE
Year : 2017  |  Volume : 13  |  Issue : 50  |  Page : 375-379

Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)


1 Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Brazil
2 Escola de Ciências Médicas, Farmacêuticas e Biomédicas, Pontifícia Universidade Católica de Goiás, 74605-010 Goiânia; Câmpus Anápolis de Ciências Exatas e Tecnológicas Henrique Santillo, Universidade Estadual de Goiás, BR-153, Km 98, 75001-970 Anápolis, GO, Brazil
3 Embrapa Hortaliças, Brazil
4 Universidade Federal de Goiás, Faculdade de Farmácia, Brazil

Correspondence Address:
Frederico S Martins
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Avenida do Café, s/n° Ribeirão Preto – SP CEP
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.210127

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Introduction: The objective of this study was to compare three capsaicin extraction methods: Shoxlet, Ultrasound-assisted Extraction (UAE), and Shaker-assisted Extraction (SAE) from Habanero pepper, CNPH 15.192. Materials and Methods: The different parameters evaluated were alcohol degree, time extraction, and solid–solvent ratio using response surface methodology (RSM). Results: The three parameters found significant (p < 0.05) were for UAE and solvent concentration and extraction time for SAE. The optimum conditions for the capsaicin UAE and SAE were similar 95% alcohol degree, 30 minutes and solid–liquid ratio 2 mg/mL. The Soxhlet increased the extraction in 10–25%; however, long extraction times (45 minutes) degraded 2% capsaicin. Conclusion: The extraction of capsaicin was influenced by extraction method and by the operating conditions chosen. The optimized conditions provided savings of time, solvent, and herbal material. Prudent choice of the extraction method is essential to ensure optimal yield of extract, thereby making the study relevant and the knowledge gained useful for further exploitation and application of this resource. Abbreviations used: Nomenclature UAE: Ultrasound-assisted Extraction; SAE: Shaker-assisted Extraction.


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