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ORIGINAL ARTICLE
Year : 2017  |  Volume : 13  |  Issue : 49  |  Page : 90-94

Osteoblasts proliferation and differentiation stimulating activities of the main components of Epimedii folium


1 The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu; Xuzhou Traditional Chinese Medicine Hospital, Xuzhou, Jiangsu, China
2 The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China
3 Xuzhou Central Hospital, Xuzhou, Jiangsu, China

Correspondence Address:
Guicheng Huang
The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.197654

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Background: Osteoporosis is a disease of bones that leads to an increased risk of fracture. Epimedii Folium is commonly used for treating bone fractures and joint diseases for thousands of years in China. Methods: This study was aimed to screen active components, which might have the potency to stimulate osteoblasts proliferation and differentiation in Epimedii Folium. An HPLC method was established to analyze the main components in Epimedii Folium. The MTT and ALP methods were utilized for the assay of osteoblasts proliferation and differentiation activity. Bavachin, a flavonoid compound was treated as the positive control. Results: Totally eight compounds have been identified by comparing their retention time with correspondent standard substances. Icariside I and icariside II significantly stimulated cell proliferation and osteoblasts differentiation. All these compounds were found with a characterized flavonoid structure in each of their molecule backbones. Conclusion: These results lead to a hypothesis that flavonoid monoglycoside structure might be crucial to exhibit the activity. The structure–effect relationship of these compounds with flavonoid monoglycoside structure in mouse primary calvarial osteoblasts needs to be explored in further research. Abbreviations used: HPLC: High performance liquid chromatography, MTT: Methylthiazolyldiphenyl - tetrazolium bromide, ALP: Alkaline phosphatase


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