Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2017  |  Volume : 13  |  Issue : 49  |  Page : 56-62

Exploratory studies of (-)-Epicatechin, a bioactive compound of Phyllanthus niruri, on the antioxidant enzymes and oxidative stress markers in D-galactosamine-induced hepatitis in rats: A study with reference to clinical prospective


1 Division of Molecular Biology and Ethanopharmacology, Department of Zoology, Sri Venkateswara University, Tirupati; Department of Zoology, T.R.R. Government Degree College, Kandukur, Andhra Pradesh, India
2 Department of Zoology, T.R.R. Government Degree College, Kandukur, Andhra Pradesh, India
3 Division of Molecular Biology and Ethanopharmacology, Department of Zoology, Sri Venkateswara University, Tirupati, India
4 Mineral Resources Research Division, Korea Institute of Geoscience, Gwahak-ro, Yuseong-gu, Daejeon, South Korea
5 Institute of Nutrition (16F), China Medical University, Hsueh-Shih Road, North District, Taichung, Taiwan

Correspondence Address:
Dr. Kondeti Ramudu Shanmugam
Department of Zoology, T.R.R. Government Degree College, Kandukur, Andhra Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.203973

Rights and Permissions

Background: Hepatitis is a health problem affecting millions of people worldwide and it is the major risk factor for liver cirrhosis. In India, many plants are used to treat hepatitis. But little is known about the effects of (-)-epicatechin a bioactive compound of Phyllanthus niruri (PN) in hepatitis rats. Objective: The present study was designed to explore the antioxidant property of (-)-epicatechin isolated from PN in D-Galactosamine (D-GalN) induced hepatitis rats. Materials and Methods: The rats are divided into five groups as per the experimental design. (-)-Epicatchin pretreatment was given to the hepatitis rats for 21 days and biochemical analysis was carried out. The hepatic antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), glutathione S-transferase (GST), reduced glutathione (GSH), and malondialdehyde (MDA) and serum markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin, and bilirubin are estimated. Results: All the antioxidant enzymes activities and albumin levels are depleted in hepatitic rats. Whereas GST, ALP, AST, ALT activities and MDA, and bilirubin levels are elevated in hepatitis rats, (-)-epicatechin pretreatment increased all the antioxidant enzymes and decreased the GST, ALP, AST, ALT, and MDA levels in hepatitis rats. However, histopatholoigic studies also proves that (-)-epicatechin pretreatment decreased the tissue damage in hepatitis condition. This is the first report on the antioxidant enzymes and hepatoprotective effect of (-)-epicatechin in hepatitis rats. Conclusion: From this study, we conclude that (-)-epicatechin treatment decreased the oxidative damage in hepatitis rats. Abbreviations used: Phyllanthus niruri (PN), D-Galactosamine (D-GalN),superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), glutathione s transferase (GST), reduced glutathione (GSH) and malondialdehyde (MDA) aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), World Health Organisation (WHO), Indian Institute of Science (IISc), Nicotinamide adenine dinucleotide phosphate (NADPH), thiobarbituric acid reactive substances (TBARS).


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1057    
    Printed30    
    Emailed0    
    PDF Downloaded16    
    Comments [Add]    

Recommend this journal