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ORIGINAL ARTICLE
Year : 2017  |  Volume : 13  |  Issue : 49  |  Page : 175-179

Rapid analysis of components in Coptis chinensis Franch by ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry


1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing; Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China
2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing, China
3 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing; Henan University of Traditional Chinese Medicine, Zhengzhou, China
4 Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding; Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China
5 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, China

Correspondence Address:
Shao-jing Li
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Dongzhimen, Beijing
China
Yu-xin Liu
College of Pharmaceutical Sciences, Hebei University, Baoding
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.197635

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Background: Coptis chinensis Franch is a traditional Chinese medical herb.Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. Materials and Methods: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode. Results: A total of 30 alkaloid and non-alkaloid components of Coptis chinensis Franch were identified in only 14 min.Conclusion: This study helped to provide a basis for the quality control of Coptis chinensis Franch. Abbreviations used: Q-TOF-MS: quadrupole time-of-flight mass spectrometry, UPLC: ultra-performance liquid chromatography, pos: positive, neg: negative.


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