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ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 46  |  Page : 384-388

Efficiency of matK, rbcL, trnH-psbA, and trnL-F (cpDNA) to molecularly authenticate Philippine ethnomedicinal Apocynaceae through DNA barcoding


1 The Graduate School, University of Santo Tomas, España, Manila 1015 and College of Arts and Sciences, Notre Dame of Dadiangas University, General Santos City 9500, Philippines
2 The Graduate School and Research Center for the Natural and Applied Sciences, University of Santo Tomas, España, Manila 1015, Philippines

Correspondence Address:
Vincent Louie Domingo Cabelin
Room 302, Plant Sciences Laboratory, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila
Philippines
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.185780

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Background: The Philippines is home to some ethnomedicinal Apocynaceae that has been used to cure common ailments. They are perceived to be safe, but misidentification can lead to substitution and adulteration. Morphological characters are primarily utilized to identify these species but a new method utilizing molecular characters called DNA barcoding has emerged. In this study, the efficiency of matK, rbcL, trnH-psbA, and trnL-F to molecularly authenticate selected Apocynaceae species were tested. Materials and Methods: Genomic DNA from silica-dried leaf samples were isolated and used as a template for generating DNA barcodes. Pair-wise sequence divergence using Kimura-2-Parameter was used to analyze inter-specific and intraspecific variations among the barcodes, whereas basic local alignment search tool (BLAST) and neighbor-joining (NJ) analyses were employed to examine discrimination success. Results: The results show that matK is the best barcode for Apocynaceae as it has the highest amplification and sequencing success together with rbcL while having high inter-specific and low intra-specific divergence relative to the other candidate barcodes. Furthermore, matK provided the highest discrimination both in BLAST and NJ analyses. Conclusion: This study proposes the use of matK as the principal barcode for Apocynaceae.


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