Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 


 
  Table of Contents  
ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 46  |  Page : 350-352  

Cocculus hirsutus: Molecular docking to identify suitable targets for hepatocellular carcinoma by in silico technique


1 Department of Pharmacognosy, PSG College of Pharmacy, Peelamedu, Coimbatore, Tamil Nadu, India
2 Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasargod, Kerala, India
3 Department of Phytopharmacy and Phytomedicine, JSS College of Pharmacy (JSS University, Mysore), Ooty, Tamil Nadu, India

Date of Submission16-Jun-2015
Date of Decision17-Aug-2015
Date of Web Publication7-Jul-2016

Correspondence Address:
B Samuel Thavamani
Department of Pharmacognosy, PSG College of Pharmacy, Peelamedu, Coimbatore, Tamil Nadu
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.185769

Rights and Permissions
   Abstract 

Background: Protein–ligand interaction plays a major role in identification of the possible mechanism by which a ligand can bind with the target and exerts the pharmacological action. Objective: The aim is to identify the best candidate of Cocculus hirsutus which binds with the hepatocellular carcinoma (HCC) targets by docking studies. Materials and Methods: The reported phytoconstituents such as coclaurine, hirsutine, cohirsine, cohirsinine, lirioresinol, cohirsitinine, haiderine, jamtinine, isotrilobine, shaheenine, jamtine, and cocsoline present in the plant, C. hirsutus were docked with the HCC targets such as Aurora kinase, c-Kit, fibroblast growth factor, nuclear factor kappa B (NF-kB), B-cell lymphoma-extra large, and vascular endothelial growth factor (VEGF) using in silico technique with the software Grid-Based Ligand Docking with Energies. Results: Haiderine, shaheenine, and coclaurine had good interaction with Aurora kinase with the glide score and glide energy of − 7.632, −7.620, −7.464; and − 56.536, −55.203, −52,822, respectively. Coclaurine, lirioresinol, and haiderine possess good binding with c-Kit with the glide score and glide energy of − 8.572, −6.640, −6.478; and − 56.527, −57.138, −20,522, respectively. Lirioresinol, hirsutine, and coclaurine exhibit good binding with c-Kit with the glide score and glide energy of − 5.702, −5.694, −5.678; and − 48.666, −35.778, −41,673, respectively. Similarly, coclaurine, haiderine, and hisutine had good interaction with NF-kB. Haiderine, jamtinine, and coclaurine had good binding with VEGF receptors (VEGFR) and coclaurine, lirioresinol, and haiderine exhibit good bonding with VEGFR. Conclusion: Coclaurine, haiderine, and lirioresinol exibited good hydrogen bonding interactions and binding energy with the select targets. Hence, these compounds have to be taken up for experimental work against hepatocellular carcinoma.

Keywords: Cocculus hirsutus, hepatocellular carcinoma, molecular docking, phytoconstituents


How to cite this article:
Thavamani B S, Mathew M, Dhanabal S P. Cocculus hirsutus: Molecular docking to identify suitable targets for hepatocellular carcinoma by in silico technique. Phcog Mag 2016;12, Suppl S3:350-2

How to cite this URL:
Thavamani B S, Mathew M, Dhanabal S P. Cocculus hirsutus: Molecular docking to identify suitable targets for hepatocellular carcinoma by in silico technique. Phcog Mag [serial online] 2016 [cited 2019 Jul 16];12, Suppl S3:350-2. Available from: http://www.phcog.com/text.asp?2016/12/46/350/185769



Summary

  • Compounds of interest showed good interaction and binding with the selected targets. Hence these compounds has to be explored further to study their anticancer potentials.



   Introduction Top


Hepatocellular carcinoma (HCC), the third common cancer, accounts for more than 626,000 new cases per year worldwide.[1] This study aimed to perform docking of ligands, which were reported in the plant Cocculus hirsutus with the macromolecular targets which involves in various apoptosis cell signaling pathways.[2] Our laboratory findings indicate that this plant is found to have in vitro cytotoxic activity in MCF-7, Hep-2, and Hela cancer cell line [3] and in vivo anticancer activity against DLA cells in mice.[4] To find out the best candidate of the plant constituents, the reported phytoconstituents of C. hirsutus were docked with the various targets responsible for different signaling pathways such as Aurora kinase, proto-oncogene c-Kit (c-Kit), B-cell lymphoma-extra large (Bcl-xL), nuclear factor kappa B (NF-kB), fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF).

Plants are rich in secondary metabolites such as alkaloids, glycosides, tannins, terpenoids, flavanoids, etc. C. hirsutus (L.) Diels is a climbing shrub belongs to the family Menispermaceae is rich in alkaloid, flavanoids, and phenolic compounds. C. hirsutus is widely used in the indigenous system of medicine for curing various ailments due to its different medicinal properties. The plants are reported to have antioxidant, cytotoxic,[5] hepatoprotective,[6] anticancer, and hypotensive.[7] The reported phytoconstituents of Cocculus hirustus are coclaurine, cocsoline, cohirsine, cohirsinine, cohirsitinine, haiderine, hirsutine, isotrilobine, Jamtine, jamtinine, lirioresinol, and shaheenine.[8],[9] Attempt has been made to dock these plant contituents with the HCC targets such as Aurora kinase, c-Kit, FGF, NF-kB, Bcl-xL, and VEGF using in silico technique with the software Grid-Based Ligand Docking with Energies (GLIDE).


   Materials and Methods Top


Previously reported phytoconstituents of C. hirsutus [Table 1] were downloaded from the database and subjected to docking studies. Maestro build panel was used for building the ligands. LigPrep is a utility of Schrodinger software (USA) that generates three-dimensional structures from two-dimensional representation. The active protein site with which the ligand will be docked was prepared using the wizard, protein preparation of Schrodinger software. Ligand and protein were docked using the software GLIDE. The prepared ligands were docked against such as Aurora kinase, c-Kit, FGF, NF-kB, Bcl-xL, and VEGF cancer targets.
Table 1: Compounds of Cocculus hirsutus taken for docking studies

Click here to view


GScore = b × Coul + a × vdW + Metal + Lipo + RotB + Hbond + BuryP + Site

Where Coul is Coulomb's energy, vdW is Van der Waal's energy, Metal is metal-binding term, Lipo is lipophilic contact term, RotB is penalty for freezing rotatable bonds, Hbond is hydrogen-bonding term, BuryP is penalty for buried polar groups, site is polar interactions at the active site, and the coefficients of vdW and Coul are a = 0.065 and b = 0.130, respectively. All docking computations were carried out using Linux is an operating system manufactured by Linus Torvalds.


   Results Top


The results of the best candidate with good binding efficiency of the phytoconstituent with good G-score, glide energy, and amino acid interactions along with the bond distance are summarized in [Table 2].
Table 2: Phytoconstituents having good interactions with cancer targets

Click here to view



   Discussion Top


From the results, it is observed that out of 12 compounds of C. hirsutus, coclaurine, haiderine, and lirioresinol are found to have good glide score and glide energy toward Aurora kinase, c-Kit, FGF, NF-kB, Bcl-xL, and VEGF.

Aurora kinase which is crucial for cell cycle control is overexpressed in tumor cells.[10] Inhibiting Aurora kinase in such increased levels will control the rapid cell growth. c-Kit receptor is present in hematopoietic cells and other tissue cells. c-Kit signaling plays a vital role in regulation of the red blood cell production, lymphocyte proliferation, etc. Downstream signal transduction regulates cell growth, proliferation, and differentiation.[11] Overexpression of Bcl-xL relates with resistance to chemotherapy and radiation therapy in multiple cancer.[12] NF-kB, i.e. NF-kB is a transcription factor, which will play a vital role in carcinogenesis. Therefore, inhibition of NF-kB activation plays a tumor suppressor role in Liver.[13] FGF target is the FGF, which will increase in solid, and metastatic tumors and gives drug resistance.[14] Thus, inhibitions of FGF will results in the entry of chemical compounds into the target cell and thereby cell toxicity and retardation of cell growth.[15] VEGF-receptors (VEGFRs) are useful of the angiogenesis (development of blood vessels from pre-existing vasculature) and vasculogenesis (development of the circulatory system). Overexpression of VEGF will lead to the abnormal growth of tumor cells.[16] Therefore, an inhibitor of VEGFR will control the growth of new cells binding with the above targets and inhibiting their activity will show a significant tumor control in liver tissues. Thus, this study suggests that these phytoconstituents, especially the best candidates such as coclaurine, haiderine, and lirioresinol, will exhibit its activity by effective binding with the targets as explained by GLIDE software [Figure 1].
Figure 1: (i). Docking of Shaheenine with Arorakinase, (ii) Docking of Coclaurine with C- kit, (iii) Docking of Lirioresinol with Bcl-xL, (iv) Docking of Coclaurine with NF-κB, (v) Docking of Jamtinine with FGF, (vi) Docking of Lirioresinol with VEGF

Click here to view



   Conclusion Top


From this study, it is clear that out of 12 compounds docked with various targets of HCC, coclaurine, haiderine, and lirioresinol have found to have good interaction and binding efficiency with all targets. Therefore, these best candidates for HCC have to be taken up for further research.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
   References Top

1.
Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, 2002. CA Cancer J Clin 2005;55:74-108.  Back to cited text no. 1
[PUBMED]    
2.
Mabrouk MS. Discovering best candidates for hepatocellular carcinoma (HCC) by in-silico techniques and tools. Int J Bioinform Res Appl 2012;8:141-52.  Back to cited text no. 2
[PUBMED]    
3.
Thavamani BS, Mathew M, Dhanabal SP.In vitro cytotoxic activity of menispermaceae plants against HeLa cell line. Anc Sci Life 2013;33:81-4.  Back to cited text no. 3
[PUBMED]    
4.
Thavamani BS, Mathew M, Palaniswamy DS. Anticancer activity of Cocculus hirsutus against Dalton's lymphoma ascites (DLA) cells in mice. Pharm Biol 2014;52:867-72.  Back to cited text no. 4
[PUBMED]    
5.
Mon MM, Maw SS, Oo ZK. Screening of antioxidant, anti-tumor and antimicrobial herbal drugs/diets from some Myanmar traditional herbs. Int J Biosci Biochem Bioinforma 2011;1:142-7.  Back to cited text no. 5
    
6.
Sagar PT, Hitesh NJ, Savita DP, Umesh MU. Hepatoprotective effect of Cocculus hirsutus on bile duct ligation induced liver fibrosis in albino wistar rats. Banglad J Pharmacol 2009;4:126-30.  Back to cited text no. 6
    
7.
Khare CP. Indian Medicinal Plants. New Delhi: Springer Science, Business Media LCC, Springer India; 2007. p. 162-3.  Back to cited text no. 7
    
8.
Viqar UA, Faryal VM, Rasheed T. Hirsudiol, a triterpenoid from Cocculus hirsutus . Phytochemistry 1987;26:793-4.  Back to cited text no. 8
    
9.
Viqar UA, Iqbal S. Jamtinine, an alkaloid from Cocculus hirsutus . Phytochemistry 1993;33:735-6.  Back to cited text no. 9
    
10.
Tatsuka M, Katayama H, Ota T, Tanaka T, Odashima S, Suzuki F, et al. Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res 1998;58:4811-6.  Back to cited text no. 10
[PUBMED]    
11.
Liang J, Wu YL, Chen BJ, Zhang W, Tanaka Y, Sugiyama H. The C-kit receptor-mediated signal transduction and tumor-related diseases. Int J Biol Sci 2013;9:435-43.  Back to cited text no. 11
[PUBMED]    
12.
Schwartz PS, Manion MK, Emerson CB, Fry JS, Schulz CM, Sweet IR, et al. 2-methoxy antimycin reveals a unique mechanism for Bcl-x(L) inhibition. Mol Cancer Ther 2007;6:2073-80.  Back to cited text no. 12
[PUBMED]    
13.
Lee CH, Jeon YT, Kim SH, Song YS. NF-kappaB as a potential molecular target for cancer therapy. Biofactors 2007;29:19-35.  Back to cited text no. 13
[PUBMED]    
14.
Korc M, Friesel RE. The role of fibroblast growth factors in tumor growth. Curr Cancer Drug Targets 2009;9:639-51.  Back to cited text no. 14
[PUBMED]    
15.
Zhao H, Liu H, Chen Y, Xin X, Li J, Hou Y, et al. Oligomannurarate sulfate, a novel heparanase inhibitor simultaneously targeting basic fibroblast growth factor, combats tumor angiogenesis and metastasis. Cancer Res 2006;66:8779-87.  Back to cited text no. 15
[PUBMED]    
16.
Saha S, Islam MK, Shilpi JA, Hasan S. Inhibition of VEGF: A novel mechanism to control angiogenesis by Withania somnifera's key metabolite withaferin A. In Silico Pharmacol 2013;1:11.  Back to cited text no. 16
[PUBMED]    

 
   Authors Top


Dr. B. Samuel Thavamani is working as associate professor in the department of Pharmacognosy, PSG College of Pharmacy, Coimbatore. He has been working in the area of phytochemical studies of plants especially of anticancer property. To his credit he has national and international publication in this area.


    Figures

  [Figure 1]
 
 
    Tables

  [Table 1], [Table 2]



 

Top
   
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
   Introduction
    Materials and Me...
   Results
   Discussion
   Conclusion
    References
    Authors
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed1037    
    Printed18    
    Emailed0    
    PDF Downloaded22    
    Comments [Add]    

Recommend this journal