Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 46  |  Page : 303-306

Storing of extracts in polypropylene microcentrifuge tubes yields contaminant peak during ultra-flow liquid chromatographic analysis


1 Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi, Karnataka, India
2 Plant Biotechnology and Tissue Culture Division; Traditional and Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi, Karnataka, India

Correspondence Address:
Sandeep R Pai
Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi - 590 010, Karnataka
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.185719

Rights and Permissions

Background and Aim: This study was designed to understand the effect of storage in polypropylene microcentrifuge tubes and glass vials during ultra-flow liquid chromatographic (UFLC) analysis. Materials and Methods: One ml of methanol was placed in polypropylene microcentrifuge tubes (PP material, Autoclavable) and glass vials (Borosilicate) separately for 1, 2, 4, 8, 10, 20, 40, and 80 days intervals stored at −4°C. Results: Contaminant peak was detected in methanol stored in polypropylene microcentrifuge tubes using UFLC analysis. The contaminant peak detected was prominent, sharp detectable at 9.176 ± 0.138 min on a Waters 250–4.6 mm, 4 μ, Nova-Pak C18 column with mobile phase consisting of methanol:water (70:30). Conclusion: It was evident from the study that long-term storage of biological samples prepared using methanol in polypropylene microcentrifuge tubes produce contaminant peak. Further, this may mislead in future reporting an unnatural compound by researchers.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed990    
    Printed11    
    Emailed0    
    PDF Downloaded22    
    Comments [Add]    

Recommend this journal