|Year : 2016 | Volume
| Issue : 46 | Page : 218-222
Pyrostegia venusta (Ker Gawl.) miers crude extract and fractions: prevention of dental biofilm formation and immunomodulatory capacity
Mayara Brito de Sousa1, José Otávio Carrera Silva2, Wagner Luiz Ramos Barbosa1, Erika da Silva Valério1, Andriele da Mata Lima3, Marlon Heggdorne de Araújo4, Michelle Frazã Muzitano4, Celso Vataru Nakamura5, Joã Carlos Palazzo de Mello5, Francisco Martins Teixeira6
1 Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil
2 Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá; Laboratory of Research and Development in Pharmaceutical and Cosmetic, College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil
3 College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil
4 Laboratory of Bioactive Products, School of Pharmacy, Federal University of Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiros - IMCT, Alcides da Conceiçã Street, 159 Novo Cavaleiros, CEP 27933-378, Brazil
5 Department of Post-Graduation Program in Pharmaceutical Sciences, Health Sciences Center, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná CEP 87020-900, Brazil
6 Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110; Pharmacy Course, Federal University of Rio de Janeiro, Campus Macaé, Av. Aluízio da Silva Gomes, 50 Granja dos Cavaleiros, CEP 27930-560, Macaé, Rio de Janeiro CEP 87020-900, Brazil
|Date of Submission||31-Mar-2015|
|Date of Decision||01-Jul-2015|
|Date of Web Publication||11-May-2016|
Francisco Martins Teixeira
Avenida Aluizio da Silva Gomes, 50 Granja dos Cavaleiros, Macae, Rio de Janeiro
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Caries and periodontal diseases remain as important diseases in the Brazilian population. One important pathogen associated with this situation is Streptococcus mutans and other important factor is this pathogen's ability to adhere firmly to the tooth surface leading to dental biofilm formation and caries development. Objectives: Determine the antibacterial and other biological activities of P. venusta related to its potential to be used in the treatment of caries and periodontal disease. Methods: The growth inhibition by P. venusta of Streptococcus mutans, S. mitis, S. oralis and Candida albicans was determined using the broth microdilution method. In addition, the effect of the samples in adherence and reducing production of acids by S. mutans, and germ-tube formation of C. albicans was analysed. The Nitric Oxide (NO) production and cytotoxicity of P. venusta to peripheral blood mononuclear cells (PBMC) and RAW 264.7 Cell Line Murine Macrophage from Blood were assessed. Results: The crude extract (CE) and ethyl-acetate (AF) and n-butanol (BF) fractions showed antibacterial activity. The ethyl-acetate (AF) fraction showed the highest inhibition percentage against the adherence of S. mutans and C. albicans cells without budding, beyond NO production inhibition. There was not any cytotoxicity in the murine macrophages RAW 264.7 cells. Conclusion: Our results suggest that P. venusta presents potential to be used as a preliminary source of compounds that can provide helpful activity when used in prophylaxis or treatment of caries or periodontal disease.
- Biological activities of Pyrostegia venusta and its potential for use in formulations for the prevention of oral diseases.
Abbreviations used: NO: Nitric oxide, PBMC: Peripheral blood mononuclear cells, CE: Crude extract, AF: Ethyl-acetate fraction, BF: n-butanol fraction, HF: Hexane fraction, WF: Water fraction, MIC: Minimum inhibitory concentration, MBC: Minimum bactericidal concentration, ATCC: American Type Culture Collection, CFU: Colony-forming units, BHI: Brain heart infusion, RPMI: Roswell Park Memorial Institute, MOPS: 3-(N-morpholino)propanesulfonic acid, DMEM: Dulbecco's modified Eagle's médium, LPS: Lipopolysacharide, MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide, OD: Optical density, AC: Acteoside,
VB: β-OH-Verbascoside, TB: Trypan blue
Francisco Martins Teixeira
Keywords: Anti-bacterial agents, Candida spp., caries, periodontal disease, Streptococcus spp
|How to cite this article:|
de Sousa MB, Silva JO, Ramos Barbosa WL, da Silva Valério E, da Mata Lima A, de Araújo MH, Muzitano MF, Nakamura CV, de Mello JC, Teixeira FM. Pyrostegia venusta (Ker Gawl.) miers crude extract and fractions: prevention of dental biofilm formation and immunomodulatory capacity. Phcog Mag 2016;12, Suppl S2:218-22
|How to cite this URL:|
de Sousa MB, Silva JO, Ramos Barbosa WL, da Silva Valério E, da Mata Lima A, de Araújo MH, Muzitano MF, Nakamura CV, de Mello JC, Teixeira FM. Pyrostegia venusta (Ker Gawl.) miers crude extract and fractions: prevention of dental biofilm formation and immunomodulatory capacity. Phcog Mag [serial online] 2016 [cited 2019 Oct 21];12, Suppl S2:218-22. Available from: http://www.phcog.com/text.asp?2016/12/46/218/182150
| Introduction|| |
Caries and periodontal disease are the most prevalent oral diseases in Brazilian population, with dental biofilm being considered the primary etiological factor in the establishment of these pathologies.Streptococcus mutans is one of the most important etiological agents of dental caries in humans, and it is considered co-responsible for the initial phase of cariogenic lesions. The adherence has a well-established role in the virulence of S. mutans since it is capable of synthesizing extracellular glucans through sucrose using glucosyltransferase enzyme. The glucans molecules have the ability to grip to various solid surfaces, which gives them the ability to adhere firmly and irreversibly to the tooth surface and can lead to dental biofilm formation and caries development.,Streptococcus oralis and Streptococcus mitis are also commonly found in dental biofilm, however, due to the absence of acidogenic or aciduric properties in these microorganisms, they are associated only with the early stages of dental biofilm formation, not acting directly on the demineralization of tooth enamel, but only making it more suitable for colonization by S. mutans.,
Another fairly common oral disease is oral candidiasis that occurs due to infection by Candida spp.Candida albicans is the yeast, most frequently isolated from the oral cavity and, in some situations, can behave as an opportunistic pathogen, for example, in situ ations of low immunity, poor oral hygiene, low salivary flow, and use of implants., The biology of C. albicans presents different aspects, including the ability to show distinct morphologies. The unicellular yeast phase can generate a bud and form hyphae. The formation of hyphae or filaments enables the cell to exert mechanical strength helping the microorganism to penetrate epithelial surfaces of the host, and once in the bloodstream, to act on the endothelium. These mechanisms allow C. albicans to insert tissues deeper into the host.
It is well established that pathogenic microorganisms are the primary causative agents of periodontal diseases. However, it is known that cytokines and inflammatory mediators can cause local tissue destruction reflecting clinically in periodontal injuries and alveolar bone loss., The antimicrobial and immunomodulatory activities of medicinal plants have been widely investigated in several experimental models to seek auxiliary or alternative strategies in the treatment of infectious and inflammatory processes in the oral cavity. The species Pyrostegia venusta (Ker Gawl) Miers, belonging to the family Bignoniaceae, is popularly known as “flor-de-São-João” or “cipó-de-São-João” and in folk medicine, it is used for the treatment of cough, bronchitis, colds, diarrhea, vitiligo, erysipelas, jaundice, and in the treatment of uterine and genital tract infections in women and female newborn.,, Consequently, due to the important biological activities attributed to P. venusta in studies at the literature not only as the melanogenic, antitumor, anthelmintic, antinociceptive, and antioxidant but also as antimicrobial and immunomodulatory agent,,,,,, the aim of this work was to evaluate the potential of crude extract (CE) and fractions from P. venusta in the prevention of major oral diseases.
| Materials and Methods|| |
P. venusta flowers were collected in Naviraí, Mato Grosso do Sul, Brazil, in June 2006. A voucher herbarium specimen was identified by Doctor Maria Auxiliadora Milaneze Gutierre and was deposited under number HUEM 11708 at the Universidade Estadual de Maringá, Paraná, Brazil. The fresh flowers were washed, dried at room temperature, and then pulverized. CE was obtained by turbo-extraction (Skymsen) of 200 g of the flower with 30% ethanol in water for 15 min. The organic solvent was eliminated by rotavapor under reduced pressure and lyophilized to yield a CE. Next, the CE (30 g) was suspended in water (300 mL) and partitioned with hexane (300 mL; HF), ethyl-acetate (AF) (300 mL; AF), and n-butanol (300 mL; n-butanol [BF]) to obtain water fraction (WF).
Determination of the minimum inhibitory concentration, minimum bactericidal concentration, and minimum fungicidal concentration
The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined according to the methods of Clinical and Laboratory Standards Institute, using samples of S. mutans (ATCC) American Type Culture Collection 25175, S. mitis ATCC 49456, S. oralis ATCC 10557, and C. albicans ATCC 0175 provided by the Instituto Nacional de Controle de Qualidade em Saúde, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. The concentrations of extracts and fractions were 1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL, 62.5 µg/mL, 31.2 µg/mL, and 15.6 µg/mL. MIC corresponded to the lowest concentration of CE and fractions able to inhibit the visible growth of microorganisms on the broth liquid medium (turbidity) with no more than 10 colony-forming units (CFU) of bacteria or fungus in the agar solid medium. The minimum bactericidal and fungicidal concentrations corresponded to the lowest concentration of CE and fractions able to inhibit the visible growth of microorganisms on the broth liquid medium associated with absence of growth on agar solid medium.,,
Glass cover slip assay
The method of Hamada and collaborators was used, partially modified. S. mutans ATCC cultured for 24 h in Brain-Heart Infusion (BHI) broth at 37°C and in microaerophilia (10% CO2) were centrifuged for 20 min and resuspended with buffer (3.0 mL) adjusted by the McFarland scale (1.5 × 108 cells/mL). From this bacterial suspension, a sample (1.5 mL) was added to buffer (control, 1.5 mL) or to CE/fractions (1.5 mL) in the MIC, previously determined. These were incubated at 37°C in microaerophilia for 4 h, centrifuged and resuspended with BHI + sucrose (3.0 mL). Serial dilutions were made in BHI medium: 10−1 and 10−2. The 10−2 dilution (500 mL) was placed on a 24 well plate with a glass cover slip. The plate was incubated at 37°C in microaerophilia for 2 h. Next, the suspension was removed from each well, and the glass coverslip was removed and rinsed with buffer to be placed into another 24 well plate. Hence, 10% sheep-blood agar in BHI (500 mL) was added on each cover slip, and the plate was incubated. CFU were counted after 24 h of incubation at 37°C in microaerophilia in triplicate. Viable colonies in dishes with 10% sheep-blood agar were also counted.,
Effect of the crude extract and fractions on the production of acids
S. mutans (1 mL of the inoculum, consisting of the equivalent to 1.5 × 108 CFU/mL – 0.5 MacFarland scale tube, was seeded in 100 mL red phenol broth containing 1% glucose and the CE/fractions) then incubated at 37°C in microaerophilia for at least 12 h and, at regular intervals of 1 h, a sample (4 mL) of the culture was removed and its pH measured with a pH meter.,
Effect on germ-tube formation and budding of Candida albicans
The previously determined MIC of extract crude and fractions from P. venusta, diluted in RPMI 1640 medium with MOPS, were tested to assess the effect on budding of C. albicans. The plates were incubated at 37°C for 48 h and observed by a negative-staining technique using 7% aqueous nigrosin in each smear by light microscopy, and the percentage of budding cells was calculated.
Murine macrophages RAW 264.7 were maintained in Dulbecco's modified Eagle's medium (DMEM) (Gibco Invitrogen Corporation, New York, USA) and supplemented with 2 mM L-glutamine, heat-inactivated 10% fetal bovine serum and 50 mg/ml gentamicin and buffered with sodium bicarbonate. The cultures were maintained at 37°C in 5% of CO2/air mixture. RAW 264.7 cells (2 × 105 cells/mL) were plated in 96-well microplates and kept in culture for 3 h for adhesion and stability of the macrophage culture. After this period, the culture supernatants were removed carefully in order to remove nonadherent cells and replaced in DMEM with or without lipopolysacharide (LPS) (1 mg/ml) (Escherichia coli 055:B5; Sigma-Aldrich, USA) in the presence or absence of CE and fractions of P. venusta at the follow concentrations 100 mg/ml, 20 mg/ml, 4 mg/ml, and 0.8 mg/ml. After 24 h, the culture supernatant was collected to evaluate the ability of CE and fractions to inhibit nitric oxide (NO) or it potential of cytotoxicity.
Nitric oxide production
NO production was indirectly estimated by measuring nitrite concentration in the supernatant through a reference curve with sodium nitrite. The supernatants (50 µL) were transferred to a new microplate and 50 µL of Griess reagent (1% sulfanilamide + 0.1 naphthylethylenediaminedihydrochloride in 5% phosphoric acid, Sigma Chemical Co.,) added, freshly prepared. After 10 min, the absorbance was measured at a wavelength of 570 nm. As a negative control, macrophages were stimulated with LPS at a concentration of 1 mg/ml and treated with nonspecific inhibitor of NO synthase NG-Monomethyl-L-Arginine (L-NMMA at 20 mg/ml) and as positive control macrophages stimulated with LPS to 1 mg/ml and untreated were used.
Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors by density centrifugation on a Ficoll-Paque Plus (GE Healthcare Biosciences, Uppsala, Sweden). PBMC (5 × 104) were incubated with CE and fractions (1000 mg/ml) in RPMI 1640 medium (Gibco, Grand Island, NY, U.S.A) supplemented with 2 mM L-glutamine, 100 U/mL penicillin, 100 mg/ml streptomycin, 10 mL/L nonessential aminoacid and 5% heat-inactivated fetal calf serum, for 1 h before the addition of 1 mg/ml LPS. After 24 h of culture, cell viability was determined by Trypan blue exclusion. In the murine macrophages, RAW 264.7 cell cultures cell viability was determined by the diphenyl tetrazolium assay-(MTT). Briefly, MTT (5 mg/mL) was dissolved in DMEM, sterilized through 0.22 mm membranes and added to the plate containing CEs or fractions plus LPS, 10 mL/well, for 4 h at 37°C in a 5% de CO2/air mixture. RAW 264.7 cells were incubated without compounds and used as viability control. Cell viability was directly proportional to OD value. The number of viable cells was expressed as a percentage relative to control cells, measured as 100% OD570, treated/OD570, control.
Data were analyzed using GraphPad Prism 4 software (GraphPad Prism Software® San Diego, California, GraphPad Software, Inc., 7825 Fay Avenue, Suite 230, La Jolla, CA 92037 USA) by analysis of one-way variance followed by multiple comparison tests (Tukey test). Results that showed P < 0.05 were considered statistically significant.
| Results and Discussion|| |
CE, AF, and BF fractions showed 1000 mg/ml MBC against all the bacterial tested but were not able to inhibit C. albicans growth on higher concentration tested (CFM > 1000 mg/ml). WF showed 1000 mg/ml MBC against S. mitis and S. oralis but was not effective against S. mutans and C. albicans. HF showed not to be effective against all the microorganisms tested, with a MBC/minimum fungicidal concentration >1000 mg/ml [Table 1].
|Table 1: Antimicrobial activity of Pyrostegia venusta crude extract, fractions and constituents |
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Some authors proposed a classification for the antimicrobial activity of plant extracts according to MIC, considering strong inhibitors (MIC down or equal to 0.5 mg/ml), moderated inhibitors (MIC between 0.6 and 1.5 mg/ml), and weak inhibitors (MIC up to 1.6 mg/ml). However, there are other authors who recommended another classification according to MIC: If the extract shows MIC lower than 100 mg/ml, the activity is considered good; if the extract shows MIC between 101 and 500 mg/ml, the activity is considered moderated; if the extract shows MIC between 501 and 1000 mg/ml, the activity is considered weak, and if the extract shows MIC greater than 1000 mg/ml, the extract is considered inactive. Our results demonstrated that CE and fractions from P. venusta flowers showed a moderate to strong activity against pathogenic microorganisms associated with caries and periodontal disease, according to the Aligiannins group classification, and they showed a weak to a moderate activity against these same pathogens when the Holetz group classification is considered, with an inhibition MIC from 500 to 1000 mg/ml when tested against the microorganisms. Taking into account that all extracts showing MIC down to 2 mg/ml could be considered having antimicrobial potential against C. albicans, our results corroborate to assume P. venusta as a good plant in the study of new molecules to treat oral diseases, and it is according with many authors who observed moderate activity in fractions of the flowers and leaves from P. venusta against Gram-Positive and Gram-Negative bacteria.,,,
Caries prevention depends on the control of microbial dental biofilm. Thus, current research aims at finding agents which have direct action on cariogenic microorganisms or that can interfere with factors involved in biofilm formation. The adhesion test result shows that all P. venusta compounds used here were able to inhibit the adherence of S. mutans to the smooth surface in the presence of sucrose, with the ethyl-acetate fraction showing the best percentage (76.7%) [Figure 1].
|Figure 1: Adherence inhibition of Streptococcus mutans when using crude extract (CE) and fractions of Pyrostegia venusta. The data represent the mean of triplicate from one of three experiments. *P < 0.0001 when compared to contril; **P < 0.01 when compared CE, AF and BF; #P < 0.01 when compared to AF|
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In association with these results, the production of acid was investigated because it is a good indicator of the fermentation of carbohydrate by bacteria and can lead to a reduction in dental plaque pH triggering demineralization of teeth and the formation of cariogenic lesions. In addition, low pH conditions promote the proliferation of aciduric and acidogenic species, including S. mutans and Lactobacillus spp., important microorganisms associated with oral diseases. Thus, one strategy for preventing caries is the use of an agent able to inhibit acid production in dental plaque.,P. venusta compounds were effective in reducing acid production by S. mutans, showing an effect comparable to that observed with the control chlorhexidine. The decrease of pH was significantly inhibited by CE and all fractions studied [Figure 2].
|Figure 2: pH values observed in Streptococcus mutans culture in red phenol broth treated with crude extract (CE) and fractions of P. venusta|
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Despite being saprophytic yeast, C. albicans can behave as an opportunistic pathogen in some situations., CE and fractions from P. venusta were able to inhibit the budding formation of C. albicans, with AF showing the highest percentage inhibition of cells from bud formation (56.3%). In contrast, WF did not show inhibition, considering its percentage was comparable to the negative control, with only 37.6% of cells without budding [Figure 3].
|Figure 3: Budding of Candida albicans was inhibited by crude extract and fractions of Pyrostegia venusta. The data represent the mean of triplicate from one of three experiments. *P < 0.0001 when compared to control; **P < 0.001 when compared to CE, HF and BF|
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Bacteria causing periodontal diseases and inflammatory cytokines can induce a higher synthesis of NO by macrophages in the periodontal tissues. The local consequence of high NO production is not only involved in the defense mechanisms of the host but also contributes to tissue damage, causing destruction in tooth supporting tissues. NO production was inhibited by AF and BF when the concentration of 100 mg/ml was used. BF was able to inhibit NO production at 20 mg/ml too. However, CE, HF, and WF were not able to inhibit NO production in the concentrations used [Figure 4]. This is promising in the research for new anti-inflammatory agents in addition to having an associated ability to control the growth of pathogenic bacteria, representing an emerging concept in the treatment of periodontitis.,,
|Figure 4: Nitric oxide production inhibition by n-butanol and ethyl-acetate fractions. The data represent the mean of triplicate from one of three experiments. *P < 0.001 when compated to control; **P < 0.001 when compared to the other concentrtions used|
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Viability of PBMC cells was lower than murine macrophage RAW264.7 cells according with the percentage of viable cells against total cells observed after culture with CE and all fractions from P. venusta stimulated with LPS. CE was the most cytotoxic, with 58.3% of PBMC cells being not viable. On the other hand, AF was the less cytotoxic, showing 76.1% of viable PBMC cells. In the murine macrophage RAW 264.7 cells, MTT assay showed a result quite different with no one of the treatments and concentrations used resulting in any cytotoxicity. This can be explained by the concentrations of the compounds used in the two assays, since in the assay by Trypan blue exclusion we used a higher concentration (1000 mg/ml) while in the MTT assay we used the 100 mg/ml concentration. In addition, our research group demonstrated previously that the cytotoxic assay by Trypan blue might present a higher number of unviable cells when compared to the MTT assay as observed in our study [Table 2].
|Table 2: Cell viability in Peripheral blood mononuclear cells (PBMC) and murine macrophages RAW 264.7 |
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Our results suggest that P. venusta presents potential to be used as a preliminary source of compounds that can provide helpful activity when used in prophylaxis or treatment of caries or periodontal disease. The ethyl-acetate fraction showed promising results justifying further studies to investigate the mechanisms of action and the possible development of a new oral antiseptic agent.
Financial support and sponsorship
Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq, Brazil.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Montandon A, Zuza E, Toledo BE. Prevalence and reasons for tooth loss in a sample from a dental clinic in Brazil. Int J Dent 2012;2012:719750.
Shivakumar KM, Vidya SK, Chandu GN. Dental caries vaccine. Indian J Dent Res 2009;20:99-106.
Sasaki EY, Ito LA, Canteli VC, Ushirobira TM, Ueda-Nakamura T, Dias Filho BP, et al.
Antioxidant capacity and in vitro
prevention of dental plaque formation by extracts and condensed tannins of Paullinia cupana
. Molecules 2007;12:1950-63.
Argimón S, Alekseyenko AV, De Salle R, Caufield PW. Phylogenetic analysis of glucosyltransferases and implications for the coevolution of mutans streptococci with their mammalian hosts. PLoS One 2013;8:e56305.
Kolenbrander PE, London J. Adhere today, here tomorrow: Oral bacterial adherence. J Bacteriol 1993;175:3247-52.
Palomer LR. Dental caries in children: a contagious disease. Rev Chil Pediatr 2006;77:50-6.
Coronado-Castellote L, Jiménez-Soriano Y. Clinical and microbiological diagnosis of oral candidiasis. J Clin Exp Dent 2013;5:279-86.
Campisi G, Pizzo G, Milici ME, Mancuso S, Margiotta V. Candidal carriage in the oral cavity of human immunodeficiency virus-infected subjects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:281-6.
Kumamoto CA. A contact-activated kinase signals Candida albicans
invasive growth and biofilm development. Proc Natl Acad Sci U S A 2005;102:5576-81.
Socransky SS, Haffajee AD. Dental biofilms: Difficult therapeutic targets. Periodontology 2012;28:12-55.
Paquette DW, Williams RC. Modulation of host inflammatory mediators as a treatment strategy for periodontal diseases. Periodontol 2000 2000;24:239-52.
Shetty S, Bose A, Sridharan S, Satyanarayana A, Rahul A. A clinico-biochemical evaluation of the role of a herbal (Ayurvedic) immunomodulator in chronic periodontal disease: A pilot study. Oral Health Dent Manag 2013;12:95-104.
Ferreira DT, Alvarez PS, Houghton PJ, Braz-Fillho R. Chemical isolated compounds from roots of Pyrostegia venusta
and considerations about its medicinal importance. Quim Nova 2000;23:42-6.
Scalon SP, Vieira MC, Lima AA, Souza CM, Mussury RM. Pregerminative treatments and incubation temperatures on the germination of “cipó-de-São-João” [Pyrostegia venusta
(Ker Gawl.) Miers]-Bignoniaceae. Rev Bras Plant Med 2008;10:37-42.
Veloso CC, Bitencourt AD, Cabral LD, Franqui LS, Dias DF, dos Santos MH, et al.
Pyrostegia venusta attenuate the sickness behavior induced by lipopolysaccharide in mice. J Ethnopharmacol 2010;132:355-8.
Roy P, Amdekar S, Kumar A, Singh V. Preliminary study of the antioxidant properties of flowers and roots of Pyrostegia venusta
(Ker Gawl) Miers. BMC Complement Altern Med 2011;11:69.
Moreira CG, Horinouchi CD, Souza-Filho CS, Campos FR, Barison A, Cabrini DA, et al.
Hyperpigmentant activity of leaves and flowers extracts of Pyrostegia venusta
on murine B16F10 melanoma. J Ethnopharmacol 2012;141:1005-11.
Silva RM, Rodrigues DT, Augustos FS, Valadares F, Neto PO, Santos L, et al.
Antitumor and cytotoxic activity of Kielmeyeracoriacea
mart. Zucc. And Pyrostegia venusta
(Ker Gawl.) Miers extracts. J Med Plants Res 2012;6:4142-8.
Nisha PV, Shruti N, Swamy KS, Kumari M, Vedamurthy AB, Krishna V, et al.
Anthelmintic activity of Pyrostegia venusta
Int J Pharm Sci Drug Res 2012;4:205-8.
Veloso CC, Bitencourt AD, Cabral LD, Franqui LS, Santa-Cecília FV, Dias DF, et al.
Anti-inflammatory and antinociceptive effects of the hydroethanolic extract of the flowers of Pyrostegia venusta
in mice. Rev Bras Farmacognosia 2012;22:162-8.
Roy P, Amdekar S, Kumar A, Singh R, Sharma P, Singh V.In vivo
antioxidative property, antimicrobial and wound healing activity of flower extracts of Pyrostegia venusta
(Ker Gawl) Miers. J Ethnopharmacol 2012;140:186-92.
CLSI. Clinical and Laboratory Standards Institute, Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts: Approved Standard M27-A2. NCCLS. Villanova, PA, USA: CLSI; 2002.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
Holetz FB, Pessini GL, Sanches NR, Cortez DA, Nakamura CV, Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz 2002;97:1027-31.
Hamada S, Torii M, Kotani S, Tsuchitani Y. Adherence of Streptococcus sanguis
clinical isolates to smooth surfaces and interaction of the isolates with Streptococcus mutans
glucosyltransferase. Infect Immun 1981;32:364-72.
Ooshima T, Osaka Y, Sasaki H, Osawa K, Yasuda H, Matsumura M, et al.
Caries inhibitory activity of cacao bean husk extract in in-vitro
and animal experiments. Arch Oral Biol 2000;45:639-45.
Ishida K, de Mello JC, Cortez DA, Filho BP, Ueda-Nakamura T, Nakamura CV. Influence of tannins from Stryphnodendron adstringens
on growth and virulence factors of Candida albicans
. J Antimicrob Chemother 2006;58:942-9.
Tada H, Shiho O, Kuroshima K, Koyama M, Tsukamoto K. An improved colorimetric assay for interleukin 2. J Immunol Methods 1986;93:157-65.
Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of the essential oils of two Origanum
species. J Agric Food Chem 2001;49:4168-70.
Duarte MC, Figueira GM, Sartoratto A, Rehder VL, Delarmelina C. Anti-Candida activity of Brazilian medicinal plants. J Ethnopharmacol 2005;97:305-11.
Ower PC, Ciantar M, Newman HN, Wilson M, Bulman JS. The effects on chronic periodontitis of a subgingivally-placed redox agent in a slow release device. J Clin Periodontol 1995;22:494-500.
Marsh PD. Are dental diseases examples of ecological catastrophes? Microbiology 2003;149:279-94.
Calderone RA, Fonzi WA. Virulence factors of Candida albicans
. Trends Microbiol 2001;9:327-35.
Lohinai Z, Benedek P, Fehér E, Györfi A, Rosivall L, Fazekas A, et al.
Protective effects of mercaptoethylguanidine, a selective inhibitor of inducible nitric oxide synthase, in ligature-induced periodontitis in the rat. Br J Pharmacol 1998;123:353-60.
Ugar-Cankal D, Ozmeric N. A multifaceted molecule, nitric oxide in oral and periodontal diseases. Clin Chim Acta 2006;366:90-100.
de Almeida MV, Teixeira FM, de Souza MV, Amarante GW, Alves CC, Cardoso SH, et al.
Thalidomide analogs from diamines: Synthesis and evaluation as inhibitors of TNF-alpha production. Chem Pharm Bull (Tokyo) 2007;55:223-6.
| Authors|| |
Dr. Francisco Martins Teixeira, is Professor at Federal University of Rio de Janeiro where he has been working with microbiological quality control and assays for evaluate biological activities of natural products. His mainly subjects of interest are good agricultural practices, microbiological analysis of plant products and water, quality control assurance.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2]