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ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 46  |  Page : 181-194  

High performance liquid chromatography-mass spectrometry analysis of high antioxidant australian fruits with antiproliferative activity against cancer cells


1 Environmental Futures Research Institute, Nathan Campus, Griffith University; School of Natural Sciences, Nathan Campus, Griffith University, Nathan, 4111 Queensland, Australia
2 School of Natural Sciences, Nathan Campus, Griffith University, Nathan, 4111 Queensland, Australia
3 Smart Water Research Centre, Griffith University, Gold Coast, 4222 Queensland, Australia

Date of Submission18-Nov-2014
Date of Decision01-Jul-2015
Date of Web Publication11-May-2016

Correspondence Address:
Ian Edwin Cock
Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Road, Nathan, Queensland 4111; School of Natural Sciences, Nathan Campus, Griffith University, 170 Kessels Road, Nathan, Queensland 4111
Australia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.182178

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   Abstract 


Background: High antioxidant capacities have been linked to the treatment and prevention of several cancers. Recent reports have identified several native Australian fruits with high antioxidant capacities. Despite this, several of these species are yet to be tested for anticancer activity. Materials and Methods: Solvent extracts prepared from high antioxidant native Australian fruits were analyzed for antioxidant capacity by the di (phenyl)-(2,4,6-trinitrophenyl) iminoazanium free radical scavenging assay. Antiproliferative activities against CaCo2 and HeLa cancer cells were determined by a multicellular tumor spheroid-based cell proliferation assay. Toxicity was determined by Artemia franciscana bioassay. Results: Methanolic extracts of all plant species displayed high antioxidant contents (equivalent to approximately 7–16 mg of vitamin C per gram of fruit extracted). Most aqueous extracts also contained relatively high antioxidant capacities. In contrast, the ethyl acetate, chloroform, and hexane extracts of most species (except lemon aspen and bush tomato) had lower antioxidant contents (below 1.5 mg of vitamin C equivalents per gram of plant material extracted). The antioxidant contents correlated with the ability of the extracts to inhibit proliferation of CaCo2 and HeLa cancer cell lines. The high antioxidant methanolic extracts of all species were potent inhibitors of cell proliferation. The methanolic lemon aspen extract was particularly effective, with IC50 values of 480 and 769 μg/mL against HeLa and CaCo2 cells, respectively. In contrast, the lower antioxidant ethyl acetate and hexane extracts (except the lemon aspen ethyl acetate extract) generally did not inhibit cancer cell proliferation or inhibited to only a minor degree. Indeed, most of the ethyl acetate and hexane extracts induced potent cell proliferation. The native tamarind ethyl acetate extract displayed low-moderate toxicity in the A. franciscana bioassay (LC50 values below 1000 μg/mL). All other extracts were nontoxic. A total of 145 unique mass signals were detected in the lemon aspen methanolic and aqueous extracts by nonbiased high-performance liquid chromatography-mass spectrometry analysis. Of these, 20 compounds were identified as being of particular interest due to their reported antioxidant and/or anticancer activities. Conclusions: The lack of toxicity and antiproliferative activity of the high antioxidant plant extracts against HeLa and CaCo2 cancer cell lines indicates their potential in the treatment and prevention of some cancers.
SUMMARY


  • Australian fruit extracts with high antioxidant contents were potent inhibitors of CaCo2 and HeLa carcinoma cell proliferation
  • Methanolic lemon aspen extract was particularly potent, with IC50 values of 480 μg/mL (HeLa) and 769 μg/mL (CaCo2)
  • High.performance liquid chromatography.mass spectrometry.quadrupole time.of.flight analysis highlighted and putatively identified 20 compounds in the antiproliferative lemon aspen extracts
  • In contrast, lower antioxidant content extracts stimulated carcinoma cell proliferation
  • All extracts with antiproliferative activity were nontoxic in the Artemia nauplii assay.


Abbreviations used: DPPH: di (phenyl)- (2,4,6-trinitrophenyl) iminoazanium, HPLC: High-performance liquid chromatography, IC50: The concentration required to inhibit by 50%, LC50: The concentration required to achieve 50% mortality, MS: Mass spectrometry.
Ian Edwin Cock

Keywords: Anticancer activity, bush tomato, desert lime, functional foods, Illawarra plum, lemon aspen, muntries, native tamarind


How to cite this article:
Sirdaarta J, Maen A, Rayan P, Matthews B, Cock IE. High performance liquid chromatography-mass spectrometry analysis of high antioxidant australian fruits with antiproliferative activity against cancer cells. Phcog Mag 2016;12, Suppl S2:181-94

How to cite this URL:
Sirdaarta J, Maen A, Rayan P, Matthews B, Cock IE. High performance liquid chromatography-mass spectrometry analysis of high antioxidant australian fruits with antiproliferative activity against cancer cells. Phcog Mag [serial online] 2016 [cited 2019 Sep 16];12, Suppl S2:181-94. Available from: http://www.phcog.com/text.asp?2016/12/46/181/182178




   Introduction Top


Reactive oxygen species (ROS) including superoxide radical (O2•−), hydrogen peroxide (H2O2), and hydroxyl radical (OH•) are products of normal cellular metabolism.[1],[2] While they may also be produced for essential biological functions, they are often by-products of incomplete reduction processes.[3] These ROS place the cell under oxidative stress and may damage cellular proteins and lipids, negatively affecting the cells chances of survival. Alternatively, ROS may also form/induce the formation of DNA adducts which may promote carcinogenic activity.[1],[4],[5] Cells respond to oxidative stress via a variety of mechanisms to minimize the effects of ROS. These defenses consist of both enzymatic and nonenzymatic mechanisms. The antioxidant defensive enzymes include superoxide dismutase, catalase, thioredoxin, thioredoxin reductase, glutathione reductase, glutathione peroxidase and glutathione reductase.[3] The nonenzymatic antioxidant defenses include glutathione, as well as vitamins A, C, and E.

High dietary intakes of nonenzymatic antioxidants have been linked with decreased incidence of some chronic diseases, including some cancers.[6] Studies into the antioxidant/prooxidant effects of extracts from various plant species have demonstrated that the ability of a plant extract to exert antioxidant activity depends on multiple factors. Aloe vera antioxidant components may function as either an antioxidant or an oxidant, with their action being dependent upon their concentration.[7] The Aloe vera anthraquinone aloe emodin exerts antioxidant behavior at lower concentrations yet acts as a prooxidant at high concentrations. In contrast, a different Aloe vera anthraquinone (aloin) has an antioxidant effect at higher concentrations, yet a prooxidant effect at low concentrations. Thus, Aloe vera extracts and components may act as either antioxidants or as oxidants, dependent on differing levels of the various constituents and their ratios. Thus, although many plant species have very high antioxidant contents, it is possible that the individual components may act as either antioxidants or as oxidants and thus may also be effective in the treatment of cancer, as well as in its prevention at different concentrations.

Similar prooxidant effects have been reported for other antioxidant phytochemicals including flavonoids [8] and tannins.[9] Previous studies have also shown that the presence of transition metal ions such as copper or iron in an extract can further enhance the conversion of the antioxidant to the prooxidant state.[10],[11] The prooxidant/antioxidant effect of plant extracts is due to a balance between the free radical scavenging activities and reducing power of their phytochemical components. This can be explained using the antioxidant vitamin ascorbic acid as an example. Although ascorbic acid has well-characterized antioxidant bioactivities, it is also known to act as a prooxidant at high concentrations.[12] This is due to the greater reducing power of ascorbic acid compared to its free radical scavenging activity. In the presence of transition metal ions, ascorbic acid will function as a reducing agent, reducing the metal ions. In this process, it is converted to a prooxidant. Therefore, high dietary intake of ascorbic acid (or other antioxidants) in individuals with high iron levels (e.g., premature infants) may result in unexpected health effects due to the induction of oxidative damage to susceptible biomolecules.[13],[14],[15]

Recent studies have documented the exceptionally high antioxidant content of the fruits of several Australian plant species.[16],[17] In particular, these studies reported the fruit of Kunzea pomifera (muntries) and Podocarpus elatus (Illawarra plum) to have similar antioxidant capacities to blueberries (which are themselves considered to have a high antioxidant capacity). Similarly, Acronychia acidula (lemon aspen), Citrus glauca (desert lime), and Solanum centrale (bush tomato) have been reported to have high antioxidant capacities.[18] It has previously been postulated that the high antioxidant contents of some Australian native fruits may provide them with therapeutic effects.[7],[16],[17],[18],[19]Terminalia ferdinandiana (Kakadu plum) has been reported to have antibacterial activity.[20] Similarly, Tasmannia lanceolata extracts have been shown to have potent antibacterial activity.[21] Recent studies have also suggested that T. lanceolata extracts may also have pro-apoptotic effects and thus, may be cytotoxic to cancer cells.[22] Several studies have also reported on the antimicrobial properties of Syzygium luehmannii and Syzygium australe fruit [23] and leaf extracts.[24],[25],[26] Recently, we reported on the antiproliferative activity of a panel of extracts prepared from selected high antioxidant Australian fruits against two cancer cell lines.[27] Despite these recent studies, reports of the anticancer activities of many of the other high antioxidant Australian plants are lacking. This study examines the antiproliferative activity of K. pomifera (muntries), P. elatus (Illawarra plum), Diploglottis australis (native tamarind), A. acidula (lemon aspen), C. glauca (desert lime), and S. centrale (bush tomato) against HeLa and CaCo2 cancer cell lines.


   Materials and Methods Top


Plant source and extraction

All fruits were obtained from taste of Australia online supplier as frozen fruits. Voucher specimens are stored in the School of Natural Sciences, Griffith University, Australia. All plant materials were thoroughly dried in a Sunbeam Food Dehydrator, and the dried plant materials were subsequently stored at −30°C. Prior to use, the plant materials were thawed and freshly ground to a coarse powder. Individual 1 g quantities of the ground fruit were weighed into tubes and 50 ml of methanol, deionized water or ethyl acetate was added. All solvents were obtained from Ajax and were AR grade. The ground fruit was individually extracted in each solvent for 24 h at 4°C with gentle shaking. The extracts were filtered through filter paper (Whatman No. 54) under vacuum, followed by drying by rotary evaporation in an Eppendorf concentrator 5301. The resultant dry extracts were weighed and redissolved in 10 ml deionized water.

Qualitative phytochemical studies

Phytochemical analysis of the extracts for the presence of saponins, phenolic compounds, flavonoids, polysteroids, triterpenoids, cardiac glycosides, anthraquinones, tannins, and alkaloids was conducted by previously described assays.[28],[29],[30],[31]

Antioxidant capacity

The antioxidant capacity of each sample was assessed using the di (phenyl)-(2, 4, 6-trinitrophenyl) iminoazanium (DPPH) free radical scavenging method [27] with modifications. Briefly, DPPH solution was prepared fresh each day as a 400 µM solution by dissolving DPPH (Sigma) in AR grade methanol (Ajax, Australia). The initial absorbance of the DPPH solution was measured at 515 nm using a Molecular Devices, Spectra Max M3 plate reader and did not change significantly throughout the assay period. A 2 ml aliquot of each extract was evaporated, and the residue resuspended in 2 ml of methanol. Each extract was added to a 96-well plate in amounts of 5, 10, 25, 50, and 75 µl in triplicate. Methanol was added to each well to give a volume of 225 µl. A volume of 75 µl of the fresh DPPH solution was added to each well for a total reaction volume of 300 µl. A blank of each extract concentration, methanol solvent, and DPPH was also performed in triplicate. Ascorbic acid was prepared fresh and examined across the range 0–25 µg per well as a reference, and the absorbances were recorded at 515. All tests were performed in triplicate, and triplicate controls were included on each plate. The antioxidant capacity based on DPPH free radical scavenging ability was determined for each extract and expressed as µg ascorbic acid equivalents per gram of original plant material extracted.

Screen for anticancer bioactivity

Cancer cell lines

The CaCo2 and HeLa carcinoma cell lines used in this study were obtained from American Type Culture Collection (Rockville, USA). The cells were cultured in Roswell Park Memorial Institute 1640 medium (Life Technologies) and supplemented with 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10 mM sodium bicarbonate, 50 µg/ml streptomycin, 50 IU/ml penicillin, 2 mM glutamine, and 10% fetal calf serum (Life Technologies). The cells were maintained as monolayers in 75 ml flasks at 37°C, 5% CO2 in a humidified atmosphere until approximately 80% confluent.

Evaluation of cancer cell antiproliferative activity

Antiproliferation of the extracts was assessed as previously described.[27],[32] Briefly, 1 ml of trypsin (Sigma) was added to the culture flasks and incubated at 37°C, 5% CO2 for 15 min to dislodge the cancer cells. The cell suspensions were then transferred to a 10 ml centrifuge tube and sedimented by centrifugation. The supernatant was discarded and the cells were resuspended in 9 ml of fresh media. Aliquots of the resuspended cells (70 µl, containing approximately 5000 cells) were added to the wells of a 96-well plate. A volume of 30 µl of the test extracts or cell media (for the negative control) was added to individual wells, and the plates were incubated at 37°C, 5% CO2 for 12 h in a humidified atmosphere. A volume of 20 µl of CellTiter 96 Aqueous One solution (Promega) was subsequently added to each well and the plates were incubated for a further 3 h. Absorbances were recorded at 490 nm using a Molecular Devices, Spectra Max M3 plate reader. All tests were performed in at least triplicate, and triplicate controls were included on each plate. The antiproliferative activity of each test was calculated as a percentage of the negative control using the following formula:

Proliferation (% untreated control) = (Act/Acc) × 100

Act is the corrected absorbance for the test extract (calculated by subtracting the absorbance of the test extract in media without cells from the extract cell test combination), and Acc is the corrected untreated control (calculated by subtracting the absorbance of the untreated control in media without cells from the untreated cell media combination).

Toxicity screening

Reference toxin for toxicity screening

Potassium dichromate (K2 Cr2O7) (AR grade, Chem-Supply, Australia) was prepared as a 1.6 mg/ml solution in distilled water and was serially diluted in artificial seawater for use in the Artemia franciscana nauplii bioassay.

Artemia franciscana nauplii toxicity screening

Toxicity was tested using a modified A. franciscana nauplii lethality assay.[33],[34],[35],[36] Briefly, 400 µl of seawater containing approximately 43 (mean 43.2, n = 155, standard deviation 14.5) A. franciscana nauplii were added to wells of a 48-well plate and immediately used for bioassay. A volume of 400 µl of diluted plant extracts or the reference toxin was transferred to the wells and incubated at 25 ± 1°C under artificial light (1000 Lux). A negative control (400 µl seawater) was run in triplicate for each plate. All treatments were performed in at least triplicate. The wells were checked at regular intervals and the number of dead counted. The nauplii were considered dead if no movement of the appendages was observed within 10 s. After 24 h, all nauplii were sacrificed and counted to determine the total % mortality per well. The LC50 with 95% confidence limits for each treatment was calculated using Probit analysis.

Nontargeted high performance liquid chromatography-mass spectrometry quadrupole time-of-flight analysis

Chromatographic separations were performed as previously described.[32] Briefly, 2 µL of sample was injected into an Agilent 1290 high-performance liquid chromatography (HPLC) system fitted with a Zorbax Eclipse Plus C18 column (2.1 mm × 100 mm, 1.8 µm particle size). The mobile phases consisted of (A) ultrapure water and (B) 95:5 acetonitrile/water at a flow rate of 0.7 mL/min. Both mobile phases were modified with 0.1% (v/v) glacial acetic acid for MS analysis in positive mode and with 5 mM ammonium acetate for analysis in negative mode. The chromatographic conditions utilized for the study consisted of the first 5 min run isocratically at 5% B, a gradient of B from 5% to 100% was applied from 5 to 30 min, followed by 3 min isocratically at 100%. MS analysis was performed on an Agilent 6530 quadrupole time-of-flight (QTOF) spectrometer fitted with a Jetstream electrospray ionization source in both positive and negative mode.

Data were analyzed using the Masshunter Qualitative analysis software package (Agilent Technologies, Australia). Blanks using each of the solvent extraction systems were analyzed using the Find by Molecular Feature algorithm in the software package to generate a compound list of molecules with abundances >10,000 counts. This was then used as an exclusion list to eliminate background contaminant compounds from the analysis of the extracts. Each extract was then analyzed using the same parameters using the Find by Molecular Feature function to generate a putative list of compounds in the extracts. Compound lists were then screened against three accurate mass databases – a database of known plant compounds of therapeutic importance generated specifically for this study (800 compounds); the Metlin metabolomics database (24,768 compounds); and the Forensic Toxicology Database by Agilent Technologies (7509 compounds). Empirical formula for unidentified compounds was determined using the Find Formula function in the software package.

Statistical analysis

Data are expressed as the mean ± standard error mean of at least three independent experiments. One-way ANOVA was used to calculate statistical significance between control and treated groups, with a P < 0.01 considered to be statistically significant.


   Results Top


Liquid extraction yields and qualitative phytochemical screening

Extraction of 1 g of the various dried Australian plant fruits with the solvents yielded dried plant extracts ranging from 3 mg (Illawarra plum fruit ethyl acetate extract) to 524 mg (muntries methanolic extract) [Table 1]. Methanolic extracts generally gave relatively high yields of dried extracted material while the aqueous extracts had moderate to high yields for most species. Extraction with chloroform also generally resulted in moderate yields. Ethyl acetate and hexane extracted lower masses for most species. The dried extracts were resuspended in 10 ml of deionized water resulting in the extract concentrations [Table 1].
Table 1: The mass of dried extracted plant material, the concentration after resuspension, qualitative phytochemical screenings and antioxidant contents of fruit extracts


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Qualitative phytochemical studies showed that methanol and water extracted the widest range of phytochemicals for both the fruits [Table 1]. All methanolic and aqueous extracts generally showed moderate to high levels of phenolics (both water soluble and insoluble phenolics), flavonoids, and saponins, as well as moderate levels of triterpenoids. Muntries and Illawarra plum also showed moderate levels of tannins. Low to moderate levels of alkaloids were also noted for the methanolic and aqueous extracts desert lime and bush tomato. The ethyl acetate extracts generally had similar phytochemical profiles as the methanolic and aqueous extracts, albeit at lower levels. Few phytochemical classes were noted in the chloroform or hexane extracts. As these tests generally screen for polar phenolic compounds, this is perhaps not surprising.

Antioxidant content

Antioxidant capacity (expressed as ascorbic acid equivalence) for the fruit [Table 1] ranged from below the level of detection to high of 15.9 mg ascorbic acid equivalence per gram of dried plant material extracted (lemon aspen fruit methanolic extract). The methanol extracts of all fruits and herbs had higher antioxidant capacities than the corresponding water, ethyl acetate, chloroform, or hexane extracts.

Antiproliferative activity

Aliquots of each extract were tested for the ability to block cell proliferation of HeLa and CaCo2 cell lines. Eighteen of the 30 fruit extracts tested displayed significant (P < 0.01) antiproliferative effects against HeLa cells [Figure 1]. A further seven extracts (lemon aspen, desert lime, and bush tomato aqueous extracts; Illawarra plum ethyl acetate extract; muntries, Illawarra plum, and desert lime hexane extracts) showed minor inhibition of HeLa cell proliferation although this inhibition was not significant (P > 0.01). All of the methanolic and chloroform extracts strongly inhibited HeLa cell growth. In contrast, only two of the aqueous extracts (muntries and Illawarra plum) significantly inhibited HeLa cell proliferation. Interestingly, three of the ethyl acetate extracts (muntries, native tamarind, and bush tomato) induced significant HeLa cell proliferation. Inhibition of proliferation was dose dependent, with the level of inhibitory activity decreasing at lower concentrations [Table 2]. The dose-dependent proliferative activity of the ethyl acetate extracts was not evaluated in this study.
Figure 1: Antiproliferative activity of plant extracts and untreated controls against HeLa cancer cells measured as percentages of the untreated control cells. 1: Muntries fruit; 2: Illawarra plum fruit; 3: Native tamarind fruit; 4: Lemon aspen fruit; 5: Desert lime fruit; 6: Bush tomato fruit; M: Methanolic extract; W: Water extract; E: Ethyl acetate extract; C: Chloroform extract; H: Hexane extract; NC: Negative control. Results are expressed as mean percentages ± standard error mean of at least triplicate determinations. *Results that are significantly different to the untreated control (P < 0.01)

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Table 2: The concentrations of extract required to achieve 50% reduction (IC50) or 25% reduction of HeLa and CaCo2 cell proliferation compared to the untreated control


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The extracts were similarly effective at inhibiting CaCo2 cancer cell proliferation [Figure 2]. Twenty-two of the 30 fruit extracts tested displayed significant (P < 0.01) antiproliferative effects against the CaCo2 cell line. As with HeLa cell proliferation, all methanolic extracts were effective inhibitors of CaCo2 cellular proliferation. Similarly, the aqueous extracts (with the exception of the native tamarind aqueous extract), ethyl acetate extracts (except the native tamarind and bush tomato extracts), and chloroform extracts were also effective inhibitors of CaCo2 proliferation. Of the hexane extracts, only the lemon aspen extract inhibited CaCo2 cell proliferation. All of the other hexane extracts (as well as the native tamarind water and bush tomato ethyl acetate extracts) induced significant CaCo2 cell proliferation. Inhibition of proliferation was dose-dependent, with the level of inhibitory activity decreasing at lower concentrations [Table 2]. The dose-dependent proliferative activity of some extracts was not evaluated in this study.
Figure 2: Antiproliferative activity of plant extracts and untreated controls against CaCo2 cancer cell lines measured as percentages of the untreated control cells. 1: Muntries fruit; 2: Illawarra plum fruit; 3: Native tamarind fruit; 4: Lemon aspen fruit; 5: Desert lime fruit; 6: Bush tomato fruit; M: Methanolic extract; W: Water extract; E: Ethyl acetate extract; C: Chloroform extract; H: Hexane extract; NC: Negative control. Results are expressed as mean percentages ± standard error mean of at least triplicate determinations. *Results that are significantly different to the untreated control (P < 0.01)

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The antiproliferative efficacy of the extracts against HeLa and CaCo2 cells was further quantified by determining the dose required to inhibit to 50% (IC50) or 25% of the control cell proliferation (IC25) [Table 2]. Lemon aspen and desert lime methanolic extracts displayed potent antiproliferative activity, with IC50 values against both cell lines <1000 µg/ml. The lemon aspen methanolic extract was particularly potent, with an IC50 value of 480 µg/mL against HeLa cells. Several other extracts (native tamarind methanolic extract, lemon aspen ethyl acetate extract, and bush tomato methanol) displayed moderate antiproliferative potency against both cell lines although their IC50 values was approximately an order of magnitude higher (IC50 <5000 µg/mL). Several extracts (lemon aspen aqueous extract, desert lime ethyl acetate extract) were potent inhibitors of only CaCo2 cell proliferation.

Quantification of toxicity

All extracts were initially screened undiluted in the assay [Figure 3]. For comparison, the reference toxin K2 Cr2O7 (1000 µg/ml) was also tested in the bioassay. The K2 Cr2O7 reference toxin was rapid in its onset of mortality, inducing nauplii death within the first 3 h of exposure and 100% mortality was evident following 4–5 h (results not shown). Similarly, most of the fruit extracts displayed >50% mortality rates at 24 h and 48 h.
Figure 3: The lethality of the undiluted fruit extracts and control (1000 µg/mL) toward Artemia nauplii. Blue and green bars represent 24 h and 48 h mortality, respectively. 1: Muntries; 2: Illawarra plum; 3: Native tamarind; 4: Lemon aspen; 5: Desert lime; 6: Bush tomato; M: Methanolic extract; W: Water extract; E: Ethyl acetate extract; C: Chloroform extract; H: Hexane extract; NC: Negative (seawater) control; PC: Positive control (1000 µg/ml potassium dichromate). All tests were performed in at least triplicate, and the results are expressed as mean ± standard error mean

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To further quantify the effect of toxin concentration on the induction of mortality, the extracts were serially diluted in artificial seawater to test across a range of concentrations in the Artemia nauplii bioassay. [Table 3] shows the LC50 values of the fruit extracts toward A. franciscana. No LC50 values are reported for the muntries aqueous and hexane extracts, the Illawarra plum and native tamarind hexane extracts or the desert lime methanolic and ethyl acetate extracts as <50% mortality was seen for all concentrations tested. All fruit extracts except native tamarind ethyl acetate extract were determined to be nontoxic with LC50 values much >1000 µg/ml following 24 h and exposure. Extracts with an LC50 of >1000 µg/ml toward Artemia nauplii have been defined as being nontoxic.[37]
Table 3: The concentrations of the fruit extracts required to achieve 50% mortality (LC50) in the Artemia franciscana nauplii assay


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High performance liquid chromatography-mass spectrometry quadrupole time-of-flight analysis

An aim of this study was to establish an HPLC-mass spectrometry (MS)/MS method for the metabolic profiling of the compounds in the bioactive extracts and to use this method to qualitatively differentiate and identify a significant number of these compounds. As the lemon aspen extracts displayed the greatest potency in the antiproliferative assay, the methanolic and aqueous methanol extracts were deemed the most promising extracts for further phytochemical analysis. Optimized HPLC-MS/MS parameters were developed and used to profile and compare the compound profiles from different extractions of lemon aspen fruit. The resultant total compound chromatograms for the positive-ion and negative-ion chromatograms of the methanolic extract are presented in [Figure 4]a and [Figure 4]b, respectively. The positive-ion chromatogram had a significantly greater number of mass signal peaks detected. However, the negative-ion chromatogram had a higher base peak signal to noise ratio in the total ion chromatograms which may have hidden some peaks in the negative ionization mode.
Figure 4: (a) Positive and (b) negative ion reverse phase-high performance liquid chromatography-mass spectrometry total compound chromatograms of 2 µl injections of lemon aspen methanolic extract

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Both the positive- and negative-ion lemon aspen fruit chromatograms of the methanolic extract revealed numerous peaks, particularly in the early and middle stages of the chromatogram corresponding to the elution of polar compounds. Nearly, all of the methanol extract compounds had eluted by 17 min (corresponding to approximately 50% acetonitrile). Indeed, multiple overlapping peaks eluted in the first 2 min with 5% acetonitrile. However, multiple peaks eluting later in the chromatogram, particularly evident in the negative ionization mode [Figure 4]b indicates the broad spread of polarities of the compounds in this extract.

The lemon aspen fruit aqueous extract in negative ionization mode [Figure 5]a also had large amounts of polar material eluting early in the chromatogram at similar elution volumes to many of the compounds in the methanol extract, although the aqueous extract in positive ion mode had a lesser amount and size of peaks corresponding to the mid polarity compounds in the middle of the chromatogram (10–20 min) at approximately 25–60% acetonitrile. Much fewer peaks were evident in the negative-ion chromatograms [Figure 5]b. The aqueous extract negative-ion chromatograms showed several major peaks, particularly at very early elution times. Many of these elution times correspond to peaks at similar elution times in the positive-ion chromatograms [Figure 5]a, indicating the corresponding compounds eluting at these times may have functional groups that are capable of both gaining and losing electrons.
Figure 5: (a) Positive and (b) negative ion reverse phase-high performance liquid chromatography-mass spectrometry total compound chromatograms of 2 µl injections of lemon aspen aqueous extract

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Qualitative mass spectral analysis of the lemon aspen fruit extracts

In total, 145 unique mass signals were noted for the lemon aspen fruit extracts [Table 4]. Putative empirical formulae were achieved for all of these compounds. Of the 145 unique molecular mass signals detected, approximately 86 compounds were putatively identified by comparison against three accurate mass databases.
Table 4: Qualitative medium-pressure liquid chromatography-mass spectrometry quadrupole time-of-flight analysis of the lemon aspen fruit methanolic and aqueous extracts, elucidation of empirical formulae, and putative identification (where possible) of the compounds


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   Discussion Top


The antiproliferative efficacy of plant extracts of six Australian fruits was examined against two cancer cell lines in vitro; HeLa (cervical) and CaCo2 (colorectal). The observed antiproliferative activity correlated with the measured antioxidant capacity. The lemon aspen and desert lime methanolic extracts (15.9 and 11.9 mg ascorbic acid equivalents per original gram of fruit extracted, respectively) were determined to have the most potent antiproliferative activity, each with IC50 values <800 µg/ml against both cell lines. The extracts which displayed moderate antiproliferative potency against both cell lines (native tamarind methanolic extract, lemon aspen ethyl acetate extract, and bush tomato methanolic extract) also had high antioxidant capacities. Indeed, antioxidant capacities of 9.2, 6.4, and 9.1 mg ascorbic acid equivalents per original gram of fruit extracted were measured for each of these extracts, respectively. Conversely, many of the extracts which had low antioxidant activity (e.g. many of the ethyl acetate and hexane extractions) lacked antiproliferative activity. Indeed, an interesting trend was noted: The lower antioxidant ethyl acetate and hexane extracts not only failed to block cancer cell proliferation but also many were observed to stimulate proliferation in both HeLa cell samples and CaCo2 cell lines.

A previous study on the antioxidant activity of fresh apples reported a similar relationship between antioxidant activity and antiproliferative activity against CaCo2 cells.[38] It was suggested that the antiproliferative activity was due to the combination of phenolic acids and flavonoids. This correlates with the results observed in the qualitative phytochemical analysis of this report. Phenolic and flavonoid compounds were observed to be present in high levels all of the extracts that displayed strong antiproliferative activity, and at much lower levels, in the extracts that did not block cell proliferation. Flavonoids are believed to protect cells from disease by shielding lipids, proteins, and DNAs from oxidative damage.[39] Previous in vitro bioactivity studies of flavonoids have demonstrated anti-inflammatory, antioxidant, and anticancer activity.[39]

HPLC-MS-QTOF analysis putatively identified a number of compounds with antioxidant activity. Hydroxyethyl salicylate [Figure 6]a,[40] ferulic acid [Figure 6]b,[41] diftalone [Figure 6]c,[42] gingerol [Figure 6]d,[43] ketorolac glucuronide [Figure 6]e,[44] rutin [Figure 6]f,[45] theophylline [Figure 6]g,[46] luteolin [Figure 6]h,[47] diosmin [Figure 6]i,[48] dihydrokaempferol [Figure 6]j,[49] ellagic acid [Figure 6]k,[50] trimethyl ellagic acid [Figure 6]l,[50] and chlorogenic acid [Figure 6]m[51] have all previously been identified as having strong antioxidant activity. The direct role of several of these compounds in anticancer mechanisms has also been demonstrated. Gingerol induces cell cycle arrest and cell death in the BxPC-3 pancreatic cancer cell line.[52] Ellagic acid induces apoptosis in HOS cells through the up-regulation of Bax and activation of caspase-3.[50] Chlorogenic acid induces cytotoxic activity against human oral squamous cell carcinoma-2 and human salivary gland tumor cell lines by activating caspase 3 and inducing nuclear condensation and DNA fragmentation.[53] Interestingly, this effect was found to be dose-dependent, with low concentrations of chlorogenic acid acting as an antioxidant and higher concentrations having prooxidant effects. Similarly, luteolin also has variable effects, functioning as either an antioxidant or a prooxidant at different concentrations.[54] That study also described several anticancer activities for luteolin including the induction of apoptosis and the inhibition of cell proliferation, metastasis, and angiogenesis.
Figure 6: Chemical structures of lemon aspen fruit compounds detected in the methanolic and aqueous extracts (a) hydroxyethyl salicylate; (b) ferulic acid; (c) diftalone; (d) gingerol; (e) ketorolac glucuronide; (f ) rutin; (g) theophylline; (h) luteolin; (i) diosmin; (j) dihydrokaempferol; (k) ellagic acid; (l) trimethyl ellagic acid; (m) chlorogenic acid; (n) (1S,5R)-4-hydroxy-6,7-dioxabicyclo[3.2.1]oct-2-en-8-one; (o) cantharidin; (p) calamanene; (q) hydroxycalamanene (r) lauroyl sarcosinate; (s) queuine; (t) dihydroartemisinin

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A number of other compounds with known anticancer activity were also putatively identified in the lemon aspen extracts. Bicyclic acetals such as (1S,5R)-4-hydroxy-6,7-dioxabicyclo[3.2.1]oct-2-en-8-one [Figure 6]n have potent cytotoxic activity toward B-cell chronic lymphocytic leukemia.[55] Cantharidin [Figure 6]o has been shown to inhibit the proliferation of pancreatic cancer cells via oxidative stress-independent cell cycle arrest and the induction of apoptosis.[56] Naphthalenes structurally similar to calamanene [Figure 6]p and hydroxycalamanene [Figure 6]q have been associated with the induction of apoptosis in human lung cancer cell lines by upregulating DR4 and DR5 cell death receptors and enhancing the activation of caspases 3, 7, 8, and 9.[57] However, other reports also indicate that naphthalenes may be mildly carcinogenic themselves.[58] Lauroyl sarcosinate [Figure 6]r may function as an antagonist of sarcosine, which is produced during prostate cancer progression, and induces the prostate cells into an invasive phenotype. Previous studies have shown that sarcosine (but not its structural isomers) induces an increase in human epithelial growth factor receptor 2 mRNA levels.[59] If lauroyl sarcosinate antagonizes the effects of sarcosine, it may have anticancer effects. Queuine [Figure 6]s administration to DLAT cancerous mice activates cellular enzymatic antioxidant defenses, blocking oxidative stress and tumorigenesis.[60] Dihydroartemisinin [Figure 6]t has recently been shown to have anticancer activity in several cell lines, including BxPC-3 and AsPC-1 pancreatic cancer cell lines [61] and ovarian cancer cells.[62] In the pancreatic carcinoma lines, dihydroartemisinin inhibited cell proliferation by down-regulating the expression of proliferating cell nuclear antigen and cyclin D1 and up-regulating p21.[61] The same study also identified several apoptotic mechanisms for dihydroartemisinin including the induction of a reduction in the Bcl-2/Bax ratio and induction of caspase-9 activity.

The findings reported here also demonstrate that the majority of the fruit extracts were nontoxic toward A. franciscana nauplii. Extracts with LC50 values >1000 µg/ml toward Artemia nauplii have been defined as being nontoxic.[37] Only the native tamarind fruit ethyl acetate extract displayed an LC50 values below 1000 µg/mL. Therefore, all other extracts were determined to be nontoxic. Despite being considered toxic, the LC50 of the native fruit ethyl acetate extract (783 µg/ml) would classify this extract as low to moderate toxicity. Furthermore, native tamarind fruits have previously been reported to have high vitamin C contents. As Artemia nauplii are susceptible to pH changes,[37] it is possible the mortality induced by these extracts is due to their high vitamin C contents. All other extracts examined in this study were nontoxic although further studies using human cell lines are required to verify the safety of these extracts for therapeutic use.


   Conclusions Top


The results of this study demonstrate the potential of several extracts prepared from high antioxidant Australian fruits to block the growth of HeLa and CaCo2 cell lines. In particular, lemon aspen methanolic and aqueous extracts were identified as particularly potent inhibitors of cell proliferation. Furthermore, these extracts displayed low toxicity in the Artemia nauplii bioassay, indicating their therapeutic potential. A number of interesting compounds with activities associated with anticancer activities were identified by LC-MS. However, the antiproliferative mechanisms of the extracts were not examined in this study. Further studies aimed at identifying the antiproliferative mechanisms of these extracts are needed.

Acknowledgments

Financial support for this work was provided by the Environmental Futures Research Institute and the School of Natural Sciences, Griffith University.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
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   Authors Top


Dr. Ian Edwin Cock leads a research team in the Environmental Futures Research Institute and the School of Natural Sciences at Griffith University, Australia. His research involves bioactivity and phytochemical studies into a variety of plant species of both Australian and international origin, including Aloe vera, South Asian and South American tropical fruits, as well as Australia plants including Scaevola spinescens, Pittosporum phylliraeoides, Terminalia ferdinandiana (Kakadu plum), Australian Acacias, Syzygiums, Petalostigmas, and Xanthorrhoea johnsonii (grass trees). This range of projects has resulted in nearly 200 scientific publications in a variety of peer reviewed journals.


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]



 

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