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ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 45  |  Page : 70-74

Optimization of process parameters and kinetic model of enzymatic extraction of polyphenols from Lonicerae flos


1 College of Light Industry and Food Sciences, South China University of Technology; College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China
2 College of Light Industry and Food Sciences, South China University of Technology; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China
3 College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China

Correspondence Address:
Shujuan Yu
College of Light Industry and Food Sciences, South China University of Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.176055

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Objective: To optimize and verify the cellulase extraction of polyphenols from honeysuckle and provide a reference for enzymatic extracting polyphenols from honeysuckle. Materials and Methods: The uniform design was used According to Fick's first law and kinetic model, fitting analysis of the dynamic process of enzymatic extracting polyphenols was conducted. Results: The optimum enzymatic extraction parameters for polyphenols from honeysuckle are found to be 80% (v/v) of alcohol, 35:1 (mL/g) of liquid-solid ratio, 80°C of extraction temperature, 8.5 of pH, 6.0 mg of enzyme levels, and 130 min of extraction time. Under the optimal conditions, the extraction rate of polyphenols was 3.03%. The kinetic experiments indicated kinetic equation C / CC0 had a good linear relationship with t even under the conditions of different levels of enzyme and temperature, which means fitting curve tallies well with the experimental values. Conclusion: The results of quantification showed that the results provide a reference for enzymatic extracting polyphenols from honeysuckle.


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