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ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 45  |  Page : 14-20

Steroidal saponin diosgenin from Dioscorea bulbifera protects cardiac cells from hypoxia-reoxygenation injury through modulation of pro-survival and pro-death molecules


1 Department of Biotechnology, Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu, India
2 Department of Biotechnology, Pondicherry University, Puducherry, India
3 Department of Pharmacology, College of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia

Correspondence Address:
A Hannah Rachel Vasanthi
Department of Biotechnology, Pondicherry University, Puducherry - 605 014
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.176114

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Background: Diosgenin, a steroidal saponin from plants, exhibits many biological potentials. Herein, the cardioprotective role of diosgenin is studied. Materials and Methods: The effect of diosgenin, isolated from Dioscorea bulbifera, was studied on hypoxia-reoxygenation (HR) in H9c2 cardiomyoblast cells. The amount of diosgenin in the plant extract was analyzed by high-performance thin layer chromatography using a solvent system comprising of chloroform:methanol:acetic acid:formic acid (13:4.5:1.5:1). Cardioprotection was checked by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Further, the release of lactate dehydrogenase, an enzyme released during cell death was checked. The proteins responsible for cell death (Bax) and cell survival (Bcl-2, hemeoxygenase-1 and Akt) were analyzed using Western blot to check the cardioprotective role of diosgenin. Conclusion:Supplementation of diosgenin mitigates HR injury, thereby exhibiting cardioprotective potential.


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