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ORIGINAL ARTICLE
Year : 2015  |  Volume : 11  |  Issue : 44  |  Page : 585-591

Phytochemical analysis on quantification and the inhibitory effects on inflammatory responses from the fruit of xanthii fructus


1 K herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Korea
2 KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon; Korean Medicine Life Science, University of Science and Technology, Daejeon, Korea

Correspondence Address:
Soo-Jin Jeong
KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon
Korea
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.172966

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Objective: Xanthii fructus (Compositae) is a traditional herbal medicine used for treating headache, toothache, pruritus, empyema, and rhinitis. In this study of the quality control of X. fructus, we performed simultaneous analysis of nine marker compounds: Protocatechuic acid (1), chlorogenic acid (2), caffeic acid (3), 4,5-dicaffeoylquinic acid (4), ferulic acid (5), 3,5-dicaffeoylquinic acid (6), 1,3-dicaffeoylquinic acid (7), 1,4-dicaffeoylquinic acid (8), and 4,5-dicaffeoylquinic acid (9). Materials and Methods: Nine components were separated using reversed-phase SunFire™ C 18 analytical column and analyzed using high-performance liquid chromatography. We examined the biological effects of the nine marker compounds by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7. Results: Among the nine marker compounds, eight significantly inhibited lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-a) production. 1, 3, 5 had significant inhibitory effects on LPS-induced prostaglandin E 2 (PGE 2 ) production in RAW 264.7 cells. None of the tested marker compounds had a significant effect on interleukin-6 production in LPS-treated RAW 264.7 cells. Our data demonstrated that each marker compound from X. fructus exerts anti-inflammatory activity by targeting different inflammation-related pathways such as the TNF-a or PGE 2 pathway. Conclusion: Further experiments using in vitro and in vivo models are needed to identify the mechanisms responsible for the anti-inflammatory properties of each marker compound.


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