|Year : 2015 | Volume
| Issue : 44 | Page : 462-468
Dosakaya Juice Assuages Development of Sucrose Induced Impaired Glucose Tolerance and Imbalance in Antioxidant Defense
Dommati Anand Kumar, Pisupati S.R Sweeya, Srishti Shukla, Sanga Venkata Anusha, Dasari Akshara, Kuncha Madhusudana, Ashok Kumar Tiwari
Medicinal Chemistry and Pharmacology Division, CSIR-n Institute of Chemical Technology, Hyderabad, Telangana, India
|Date of Web Publication||16-Nov-2015|
Ashok Kumar Tiwari
Medicinal Chemistry and Pharmacology Division, Academy of Scientific and Innovative Research, CSIR-Indian Institute of Chemical Technology, Hyderabad - 500 007, Telangana
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Objective: The objective was to explore the effect of Dosakaya (DK) (Cucumis melo var. chito) juice on sucrose induced dysglycemia and disturbances in antioxidant defense in rats. Materials and Methods: Rats were preconditioned with DK juice before administration of sucrose beverage continuously for 1-month. Blood glucose tolerance test and glutathione (GSH) homeostasis pathways in kidney were analyzed in different group of animals at the end of the study. Results: DK juice diffused (P < 0.001) hypertriglyceridemia inducing effect of sucrose and arrested sucrose induced weight gain. It improved glucose tolerance ability by significantly reducing (P < 0.05) first-hour glycemic excursion and decreasing 2 h glycemic load (P < 0.05) following oral glucose tolerance test in sucrose fed animals. Furthermore, disturbances in antioxidant defense mechanisms in terms of GSH homeostasis in kidney were restored due to juice feeding. DK juice administration checked reduction in GSH-S-transferase and glyoxalase-I activity, thus, significantly mitigated lipid peroxidation (P < 0.05), and formation of advanced glycation end-products (P < 0.001) in kidney and serum (P < 0.01). Quantitative analysis of juice found it a rich source of protein and polyphenols. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed the presence of multiple protein bands in whole fruit juice. Therefore, SDS-PAGE protein fingerprint of DK juice may serve as a quality control tool for standardization of juice. Conclusion: The whole fruit juice of DK may become cost-effective, affordable health beverage in extenuating ill-health effects of sugar consumption. This is the first report identifying DK juice in preventing development dysglycemia, dyslipidemia, and oxidative stress induced due to chronic sucrose feeding in rats.
Keywords: Antioxidant activity, Dosakaya juice, glycemic excursion, impaired glucose tolerance, oxidative stress, protein-fingerprint, sucrose feeding, type 2 diabetes
|How to cite this article:|
Kumar DA, Sweeya PS, Shukla S, Anusha SV, Akshara D, Madhusudana K, Tiwari AK. Dosakaya Juice Assuages Development of Sucrose Induced Impaired Glucose Tolerance and Imbalance in Antioxidant Defense. Phcog Mag 2015;11, Suppl S3:462-8
|How to cite this URL:|
Kumar DA, Sweeya PS, Shukla S, Anusha SV, Akshara D, Madhusudana K, Tiwari AK. Dosakaya Juice Assuages Development of Sucrose Induced Impaired Glucose Tolerance and Imbalance in Antioxidant Defense. Phcog Mag [serial online] 2015 [cited 2020 Jan 21];11, Suppl S3:462-8. Available from: http://www.phcog.com/text.asp?2015/11/44/462/168985
- Chronic sucrose consumption induced development of dysglycemia and also impaired antioxidant defense mechanism in rats. The oral administration of Dosakaya juice prior to sucrose feeding however, mitigated the development of dysglycemia and impairment in antioxidant defense in rats.
| Introduction|| |
Despite the increase in understanding disease pathobiology and advances made in the field of medical sciences, modern world is still being engulfed by diseases of metabolic overload such as type 2 diabetes mellitus (T2DM), obesity, hypertension, cardiovascular disorders, and kidney diseases. Although, the consumption of high-caloric processed food items and decrease in physical activities are recognized as important factors driving such epidemics, the paradigm shift in research on food, nutrition, and health finds that increased consumption of sucrose (sugar) loaded food products and sugar-sweetened beverages in modern world further inflates the risk of diabetes, metabolic syndrome, and cardiovascular disorders. It has been reported recently that sugar consumption independently aggravates the development of chronic diseases linked to metabolic perturbations involving dysglycemia, dyslipidemia, and insulin resistance. It has also been observed that per capita sugar consumption is positively and independently associated with an increasing prevalence rates of T2DM worldwide and Asian regions in particular. Therefore, International Scientific Advisory Committees on Nutrition, recommend limiting sugar consumption to 5% of daily calories to curb and or delay the development of diseases linked with metabolic overload.
On the other hand, researches dealing with food and food eating habits are finding that eating vegetables before carbohydrate-rich meals significantly reduces postprandial glycemic load, burden on pancreatic β cells to secrete insulin, improves postprandial glycemic excursions, and is helpful in long-term glycemic control in T2DM patients., Disclosing the health benefits of vegetable consumption, researchers find that they are rich natural sources of biological antioxidants and possess capabilities of maintaining glucose homeostasis by diverse mechanisms.
Dosakaya (DK) (Cucumis melo var. chito, Family: Cucurbitaceae) is cultivated prominently in the Southern parts of India. The fruit of DK, also known as Mother Mary's Pie Melon, is used by common people to prepare culinary items ranging from curries to pickles. It is a rich source of protein, polyphenols, and flavonoids, display multiple antioxidant activities, moderate starch-induced postprandial glycemic load in rats, inhibit protein-tyrosine phosphatase 1β enzyme responsible for the development of insulin resistance, and influence polyol pathway by multiple mechanisms that may help prevent the development of diabetic complications. However, studies on chronic feeding of DK in dysglycemic animal models are not yet performed. In the present research, we report the effect of DK juice on the development of sugar induced dysglycemia and influence on antioxidant defense mechanisms in rats. Simultaneously, we also present sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) based protein fingerprint of whole DK fruit juice as a tool for standardization of the vegetable's juice along with quantitative analysis of total polyphenol, protein contents.
| Materials and Methods|| |
1-chloro-2,4-dinitrobenzene (CDNB), 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), thiobarbituric acid (TBA), nicotinamide adenosine dinucleotide phosphate (NADPH), acetic acid, oxidized glutathione (GSSG) and reduced glutathione (GSH), methyglyoxal and Folin–Ciocalteu phenol, and Bradford's reagents were purchased from Sigma-Aldrich Chemical Co. (St. Louis, Mo. USA). Other reagents of analytical grade were purchased from Indian manufacturers.
Sugar solution and Dosakaya juice preparation
The sugar solution was prepared daily in distilled water and orally administered to rats at a dose of 1.5 g/kg body weight. DK fruits were purchased from the local vegetable market. Fruits of Cucurbitaceae family sometimes taste bitter; therefore, pieces of DK fruit were self-tested and discarded if bitter. The raw DK fruit is delicious in nature and does not taste sweet. A paste of chopped pieces of DK fruit including seeds was made in food grade grinder and squeezed to get raw juice on a clean sterilized muslin cloth. Juice was orally administered to rats a fresh at 7.5 mL/kg body weight dose  daily 30 min before sucrose feeding. For phytochemical analysis and SDS-PAGE protein-fingerprint, a paste of seeds was also prepared and after centrifugation, the supernatant was used for analysis.
Adult male Wistar albino rats (180–220 g body weight) were purchased from Gentox Bio Services, Hyderabad, India. Rats were quarantined and acclimatized in institute's animal house (temperature 24 ± 2°C, relative humidity 40–60%, and 12 h light/dark cycle). Normal rat chow pellets (Nutrimix Sp-1620, Nutrivet Life Sciences, Pune, India) and drinking water were provided ad libitum. Animal experiments were conducted following the ethical norms and CPCSEA guidelines. The experimental protocol was approved by Institutional Animal Ethical Committee (CPCSEA Reg. No. 97/1999 Government of India). Experiments with live animals comply relevant laws, and institutional guidelines and animals were handled humanly.
Rats were kept on overnight fasting and following day (forenoon) blood was collected from retro-orbital plexus in EDTA containing tubes. Plasma glucose levels ("0" h) were measured by glucose-oxidase test (Siemens Healthcare Diagnostics Kit, Newark, USA) on auto-blood analyzer (Dimension Xpand plus Clinical Chemistry System, Siemens, Germany).
Rats were grouped (six rats in each group) based on their blood glucose levels as follows:
- Sugar control: Single dose 1.5 g/kg body weight sucrose solution per oral daily for a period of 1-month at forenoon time
- DK juice + sugar: Single dose (7.5 mL/kg body weight) freshly prepared DK juice followed by single dose (1.5 g/kg body weight) sucrose solution per oral was administered for a month. The gap between juice and sucrose feeding was 30 min
- Normal control: Rats were administered normal saline and treated sham.
Rats were kept in polypropylene rat cages with sterilized corn bedding. Food and water were provided ad libitum throughout the study.
Oral glucose tolerance test
At the end of 30 days experimental period, rats were kept for overnight fasting. The next morning "0" h blood samples were collected for estimation of hematological parameters, glucose, total cholesterol, and triglycerides. Hematological parameters were analyzed using Siemens Health care Diagnostic Kits (Newark, USA) on Advia 2120i Hematology analyzer (Siemens, Germany). Plasma glucose, total cholesterol, and triglycerides were analyzed using Siemens Healthcare Diagnostics Kits on the auto-blood analyzer. Glucose solution at the dose of 2 g/kg body weight was administered to all the rats. Blood was drawn at the intervals of 30 min for up to 120 min, and plasma glucose levels were estimated. Two-hour postprandial glycemic load  and delta-glucose (ΔG) values  were calculated accordingly.
Animals were euthanized under CO2 anesthesia after completion of the experiment. Relevant organs were surgically removed, cleared of adventitious tissues, washed, and stored in −80°C Freezer (NUAIRE-2100, Ultraflow freezer, Plymouth, MN, USA) for further analysis.
Enzymatic and biochemical analysis
Kidney tissues (50 mg) were chopped and homogenized (Heidolph Schwabach, Germany) in 500 µL of PBS (0.1 M, pH 7.2) with the addition of cocktail protease inhibitor. Centrifugation (Beckman Coulter Avanti J-301 centrifuge, California, USA) was done at 15,000 rpm for 30 min at 4°C. The supernatant was stored at −80°C for analysis.
Estimation of reduced glutathione
GSH was measured in the kidney following Ellman method. A volume of 100 µL supernatant was reconstituted in 2 mL of phosphate buffer (0.3 M, pH 8.0), 500 µL DTNB (2 mg DTNB + 1 g sodium citrate dissolved in 100 mL water), mixed thoroughly and incubated for 10 min. The intensity of yellow color was measured spectrophotometrically (Perkin Elmerprecisely Labda 25, UV-Vis Spectrophotometer, Waltham, MA, USA) at 412 nm. Results were expressed as mM.
The method for estimation of glutathione reductase (GR) was adapted from Carlberg and Mannervick. The kinetics of reaction mixture containing 150 µL of GSSG (2 mM), 50 µL of assay buffer (0.1 M KH2 PO4 + 1 M EDTA, pH 7.0), 30 µL sample, and 20 µL NADPH (2 mM) in a 96-well plate was read at 340 nm for 3 min at 30 s interval spectrophotometrically (BioTek synergy4 Multimode Microplate Reader, BioTek Instruments Inc., Winooski, VT, USA). NADPH utilization was considered as a measure of enzyme activity. The results were expressed as ΔOD/min.
Glutathione-S-transferase (GST) method was applied for measurement of GST activity. Twenty microliter supernatant was reconstituted in reaction mixture containing 150 µL of phosphate buffer (0.1 M, pH 6.5, 20 µL of GSH (1 mM) and 10 µL of CDNB (1 mM) in a 96-well plate. Reaction kinetics was recorded every 1 min for 5 min at 340 nm spectrophotometrically. Results were expressed as ΔOD/min.
Glutathione peroxidase analysis
Glutathione peroxidase (GPx) activity in kidney supernatant was measured following the method of Rotruck et al. with suitable modifications. Briefly, 100 µL each of GSH (4 mM), sodium azide (10 mM), EDTA (0.8 mM), and hydrogen peroxide (30 mM) were incubated for 10 min at 37°C in the presence of 20 µL of respective kidney supernatant. The mixture was centrifuged at 3000 rpm for 10 min after addition of 500 µL trichloroacetic acid (10%). Hundred microliters DTNB (0.04%) was added to the supernatant. Absorbance was recorded at 420 nm and expressed as enzyme activity.
Assay of glyoxalase-I activity
Glyoxalase-I (Glo-I) activity was assayed as described by Thornalley  with suitable modifications. In brief, hemithioacetal was prepared by mixing 500 µL of 2 mM GSH in sodium phosphate buffer (100 mM, pH 6.6) with 500 µL methyglyoxal (2 mM) and incubated for 10 min at 37°C. The reaction was started by addition of 20 µL kidney supernatant as a source of enzyme. The conversion of hemithioacetal into S-D-lactoylglutathione due to Glo-I was measured spectrophotometrically (240 nm). Results were expressed as an increase in absorbance as a function of enzyme activity.
Analysis of thiobarbituric acid reactive substances
Thiobarbituric acid reactive substances as a measure of lipid peroxidation products were measured as described by Wills. In 5 mL glass test tube, 100 µL supernatant, 200 µL sodium dodecyl sulfate (8.1%), 1.5 mL acetic acid (30%, pH 3.5), 1.5 mL TBA were mixed thoroughly and incubate in boiling water-bath for 60 min at 95°C and cooled. Two hundred microliter clear solution was transferred to a 96-well plate, and absorbance (535 nm) was recorded spectrophotometrically. Results were expressed as nM/g tissue.
Advance glycation end-products analysis 150 mg chopped kidney tissues were soaked in chloroform-methanol (2:1) mixture and incubated (4°C) overnight in shaker incubator (Innova 4230 refrigerated incubator shaker, New Brunswick Scientific, Champaign, IL, USA). Filtered (0.2-micron filter) pellets were washed thrice with methanol and water. Tissues were homogenized in 1 mL NaOH (0.1 N) and centrifuged at 8000 rpm for 15 min at 4°C. Fluorescence (excitation 370 nm and emission 440 nm) was recorded. Results were expressed in fluorescent units.
Analysis of advanced glycation end-products in serum
Serum was deproteinized by mixing with an equal volume of acetonitrile and incubated for 2 min (−20°C) and centrifuged for 10 min at 9500 rpm (4°C). Fluorescent advanced glycation end-products (AGEs) in the supernatant were assayed (excitation 370 nm and emission 440 nm) as reported earlier. Results were expressed in fluorescent units.
Estimation of total polyphenols A volume of 25 µL supernatants from whole fruit juice or the seeds was diluted with 2.5 mL distilled deionized water. Equal volumes of Folin–Ciocalteu reagent and Na2 CO3 were mixed to this and incubated for 60 min. Absorbance (765 nm) was recorded, and results were expressed as μg gallic acid equivalent/mL as described earlier.
Estimation of total protein Protein content in juice, as well as seeds, was determined using Bradford's dye as described earlier. Briefly, 10 µL samples were reacted with 240 µL of ×1 Bradford reagent, and absorbance (595 nm) was recorded. Protein concentration was expressed BSA equivalent/mL.
Sodium dodecyl sulfate polyacrylamide gel electrophoresis protein-fingerprinting of whole juice and seeds Protein-fingerprint was prepared as described earlier  with suitable modifications. Supernatant of whole fruit and seed of DK were mixed with ice-cold acetone (100% v/v) and trichloroacetic acid (100% w/v) in the ratio of 1:8:1 and kept at −20°C for 1 h for protein precipitation. Pellet was collected after centrifugation at 15,000 rpm at 4°C for 15 min. Pellet was suspended in 1 mL ice-cold acetone and centrifuged again at 15,000 rpm 4°C for 15 min. This step was repeated three times to remove the trichloroacetic acid. Pellet was dried to remove acetone. Dried protein pellets were dissolved in PBS. Protein concentration was measured using Bradford's reagent. Forty microgram protein was mixed with 7 µL of nonreducing (×4) lithium dodecyl sulfate sample buffer (Thermo Scientific, USA) and heated at 95°C for 5 min in dry bath and cooled at room temperature. Twenty micro gram protein were loaded on 10% SDS-PAGE (Bio-Rad protein gel apparatus) along with 5 µL molecular weight marker (Biomatik, USA). The gel was run in trisglycine buffer, at constant voltage (120 V). Coomassie brilliant blue (0.5%, 250 R) stained gel was de-stained several times with methanol: acetic acid: water (40:10:50) mixture and scanned with scanner (HP LaserJet M1136 MFP, USA)
A one-way analysis of variance followed by Tukey's multiple comparison tests was applied to find differences in study groups. The criterion for statistical significance was P < 0.05. Statistical analyses were performed using GraphPad PRISM® version 5.01 (La Jolla, USA).
| Results and Discussion|| |
Mortality due to feeding sugar alone or DK juice + sugar was not found during the study period. Rats in normal and DK juice + sucrose were active throughout the study, however, animals in sucrose fed group looked sluggish 15 days onward.
Hematological and biochemical observations in blood
DK juice, as well as sucrose feeding for a month, did not bring any significant changes in blood cells and related parameters [Table 1]. Although, an increase in body weight was found in sucrose fed animals, it was not statistically significant. Similarly, no significant changes in fasting plasma glucose and total cholesterol levels were noticed at the end of the study. However, the plasma triglyceride levels were significantly (P < 0.01) increased in rats receiving sucrose when compared with a normal group of rats. Fasting plasma triglyceride levels in rats receiving DK juice before sucrose feeding was found significantly (P < 0.05) lesser when compared with a normal group of animals and sucrose fed rats (P < 0.001). Results indicate that DK juice possess anti-hypertriglyceridemic activity by defusing hypertriglyceridemic effects of sucrose and also possess hypotriglyceridemic activity since it reduced triglyceride levels below normal values [Table 1].
|Table 1: Values of hematological and biochemical parameters of rats at the end of the study|
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Oral glucose tolerance test
The shape of plasma glucose concentration curve following oral glucose tolerance test (OGTT), foretells risk of T2DM development in a person in future. Based on the shape of plasma glucose concentration curve following OGTT, it has been found that if plasma glucose concentration does not return to basal level, person may bear the characteristics of impaired glucose tolerance (IGT), insulin resistance, compromised insulin sensitivity, and hence, individual is considered as a case of prediabetes. Prediabetes state often progresses to overt diabetes within few years. Therefore, an early lifestyle modifications can reverse back prediabetes state to normal state and attenuate diabetes progression. Furthermore, it is advised to maintain postprandial glucose curve flat  because postprandial hyperglycemia (PPHG) is one of the earliest detectable abnormalities in diabetes prone individuals.
Our research clearly demonstrates that continuous administration of sucrose for 1-month induced IGT development in rats [Figure 1]a. Interestingly, however, prior administration of DK juice substantially curtailed this effect and significantly (P < 0.05) reduced postprandial glycemic load following OGTT [Figure 1]d.
|Figure 1: Oral glucose tolerance test in rats of different experimental groups at the end of 30 days experimental period. (a) The shape of plasma glucose concentration curve at different time points following oral glucose tolerance test. (d) Glycemic load (area under the curve, AUC) following oral glucose tolerance test. **P< 0.01 normal versus sucrose, *P< 0.05 sucrose versus Dosakaya + sucrose. (b) Glycemic spikes during the first hour (ΔG60–0 min) following oral glucose tolerance test. *P< 0.05 normal versus sucrose, **P< 0.05 sucrose versus Dosakaya + sucrose. (c) Glycemic spikes between 60 and 120 min (ΔG60–120 min) following oral glucose tolerance test. Values represent mean ± standard error of mean, n = 5−|
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Glucose fluctuations during the postprandial period are recognized to trigger oxidative stress. Slama et al. proposed that postprandial glycemic excursion plays an important role in total hyperglycemia. Therefore, the measurement of Δ-postprandial glycemia (ΔG) is a more useful tool than the conventional examination of absolute rise in the postprandial blood glucose level. Furthermore, control of acute glucose surge is emerging as a new therapeutic tool toward minimizing hyperglycemia-induced oxidative stress and progression of diabetic complications.
The first-hour glucose surge (ΔG60–0 min) following OGTT is presented in [Figure 1]b. Glycemic spikes during the first hour were significantly (P < 0.05) higher in sucrose fed animals than in a normal group. On the other hand, the first-hour glycemic surge was significantly (P < 0.05) lesser in rats receiving DK juice along with sucrose and was close to the normal group of rats. Contrarily however, in the later half (ΔG60–120 min), the decrease in blood glucose level following OGTT in DK group was lesser than the reductions observed in rats receiving only sucrose [Figure 1]c. This reduction was prominent in the normal group of rats and blood glucose level reached close to basal values [Figure 1]a. Wide individual variations in blood glucose surge and deceleration were noticed in rats fed with sucrose and DK juice + sucrose following OGTT. These variations may arise due to their individual responsiveness and susceptibility to sucrose treatment, as well as DK juice.
PPHG is an important factor in increasing free radicals generation and oxidative stress even in nondiabetic individuals. Accelerated generation of free radicals and consequent oxidative stress is an important link between diabetes and development of cardiovascular diseases. Prolonged and high consumption of sucrose and fructose induces development of oxidative stress. Therefore, arresting development of oxidative stress due to hyperglycemia presents exciting therapeutic opportunity in mitigating onset of diabetic complications.
Furthermore, antioxidant defense in body is governed by complex mechanisms working in coordination with a number of pathways. For example, GSH works in coordination with other redox-cycles operating in the body to maintain and regulate cellular redox balance [Figure 2]. GSH performs multiple functions and displays antioxidant activities in various ways. Therefore, maintenance of GSH homeostasis is an important consideration for the balanced antioxidant defense.
|Figure 2: Scheme represents glutathione homeostasis. The reduced glutathione and oxidized glutathione forms of glutathione work in coordination with other redox-cycles (e.g. nicotinamide adenosine dinucleotide phosphate) to maintain and regulate cellular redox balance. Glutathione reductase reduces glutathione disulfide to sulfhydryl form (glutathione) by nicotinamide adenosine dinucleotide phosphate-dependent mechanism. Nicotinamide adenosine dinucleotide phosphate is primarily available via pentose phosphate pathway involved in glucose oxidation. Glutathione acts directly as antioxidant and also as cofactor for various enzymes such as glutathione–S-transferase responsible for protection against various genotoxic and carcinogenic compounds, glutathione peroxidase accountable for reduction of various peroxides (LOOH, H2O2) and glyoxalases involved in elimination of advanced glycation end-products). An imbalance in the activities of antioxidant enzymes such as glutathione reductase, glutathione peroxidase, glutathione–S-transferase), glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase may affect cellular defense system. NADP +: Nicotinamide adenine dinucleotide, LOOH: Lipid peroxides|
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A significant (P < 0.05) increase in GSH concentration was recorded in kidney tissues of rats receiving DK juice along with sucrose when compared with normal rats [Figure 3]a. GSH is regenerated from oxidized GSSG by the enzyme GR in coordination with NADPH cycle primarily originating from the pentose phosphate pathway [Figure 2]. Results presented in [Figure 3]b show that although the GR activity was augmented in sucrose fed rats, this increase was significantly (P < 0.05) higher than normal rats in animals receiving DK juice along with sucrose [Figure 3]b. The augmentation in GR and GSH levels in sucrose fed animals than normal group of rats may be linked to the energetic system related to pentose phosphate pathway, whereas increased glucose concentration due to sucrose feeding might be providing enough NADPH through the pentose phosphate pathway [Figure 2]. As, DK juice is considered as rich source of antioxidants,, significant increase in levels of antioxidants GR and GSH in DK juice + Sucrose group of animals appears advantage of DK juice feeding [Figure 3]a and [Figure 3]b. The increase in GR levels due to DK juice in rats can be seen as an improvement in antioxidant defense because; GR protects cells from harmful effects of hydroperoxides.
|Figure 3: The effect of Dosakaya juice on various antioxidant parameters related to glutathione homeostasis in kidney under the influence of sucrose feeding. (a) Concentration of reduced glutathione in various experimental groups, *P< 0.05 normal versus Dosakaya + sucrose. (b) The level of glutathione reductase, *P< 0.05 normal versus Dosakaya + sucrose. (c) The level of Glutathione-S-transferase, *P< 0.05 normal versus sucrose. (d) The activity of glutathione peroxidase. (e) The activity of glyoxalase-I (Glo I), *P< 0.05 normal versus sucrose. (f) Thiobarbituric acid reactive substances (TBARS) as measure of lipid peroxides in kidney, *P< 0.001 normal versus sucrose, **P< 0.05 sucrose versus Dosakaya + sucrose, ***P< 0.05 normal versus Dosakaya + sucrose. (g) Advanced glycation end-products (AGEs) in kidney, *P< 0.001 normal versus sucrose, **P< 0.001 sucrose versus Dosakaya + sucrose. (h) Advanced glycation end-products (AGEs) in serum, *P< 0.01 normal versus sucrose, **P< 0.01 sucrose versus Dosakaya + sucrose. Data represent mean ± standard error of the mean of 5–6 animals. Readings were taken in triplicate for each animal|
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Enzymes GST and GPx are responsible for the protection against various genotoxic agents and peroxide radicals in the cells. A significant decrease (P < 0.05) in GST level from normal levels in sucrose fed rats was observed [Figure 3]c in our study. However, preconditioning of rats by DK juice feeding prevented the reduction in GST level due to sucrose, in kidney tissues [Figure 3]c. We did not observe appreciable changes in GPx level in our study [Figure 3]d.
GSH also acts as a cofactor in systems responsible for the elimination of AGEs in association with glyoxalases. Thornalley  reported decreased activity of Glo-I in hyperglycemic conditions. The decrease in Glo-I activity, therefore, may compromise elimination of AGEs and promote their accumulation. A significant decrease (P < 0.05) in Glo-I activity after sucrose feeding was noticed in our study also [Figure 3]e. However, the level of Glo-I was maintained close to the normal in rats receiving DK juice before sucrose [Figure 3]e. These observations find that DK juice prevents imbalance in antioxidant homeostasis induced due to sucrose feeding and may help prevent damage to biomolecules and their accumulation.
Reduction in GST and Glo-I activities due to sucrose feeding might be held responsible for increased levels of lipid peroxides [Figure 3]f and AGEs in the kidney [Figure 3]g. For DK juice feeding retained activities of GST and GPx, so the values of lipid peroxides were recorded significantly (P < 0.05) lesser [Figure 3]f. However, it was still higher (P < 0.001) than normal animals.
Hyperglycemia and accompanied oxidative stress accelerate formation of AGEs. Increased generation and accumulation of AGEs are responsible for aggravating development of diabetic complications. Significant increase in AGEs were recorded in kidney ([Figure 3]g, P < 0.001), as well as in serum ([Figure 3]h, P < 0.01), of sucrose fed rats than the level recorded in normal group of rats [Figure 3]g and [Figure 3]h. These observations find support from our earlier observations that sustained hyperglycemia induces oxidative stress and increases the formation of AGEs. The DK juice significantly prevented the formation of AGEs, both in kidney ([Figure 3]g, P < 0.001), as well as in serum ([Figure 3]h, P < 0.01).
Phytochemical analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis based protein-fingerprints
Polyphenols and proteins present in plant materials are reported to possess antioxidant activities., Furthermore, a high protein concentration in vegetables is held responsible for the anti-hyperglycemic activity. Our analysis finds that whole fruit juice, as well as seeds of DK, is rich in polyphenols and proteins. The polyphenol and protein concentration in seeds were recorded significantly higher (P < 0.05 and P < 0.001, respectively) than in whole fruit juice [Figure 4]a.
|Figure 4: Phytochemical analysis in whole juice and seeds of Dosakaya. (a) Total polyphenol (PL) and protein content (PR) in whole juice (wj) and seeds (s), *P< 0.05 wj versus s and **P< 0.001 wj versus s values represent mean ± standard error of mean of at least three experiment. (b) Sodium dodecyl sulfate polyacrylamide gel electrophoresis protein fingerprint of wj and s of Dosakaya|
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One of the major challenges associated with natural products is lack of suitable standardization technique leading increase in adulteration malpractices. The unique banding pattern of protein electrophoregram of individual food material can provide biometric data for their identification and standardization of products., The SDS-PAGE protein-fingerprint [Figure 4]b of whole fruit, as well as seeds of DK, may serve this purpose.
Changes in lifestyle and dietary patterns from traditional to modern are being held responsible for the outburst of hyperglycemia and hyperlipidemia in modern society. Therefore, simple realignment of our dietary habits with natural food materials may provide holistic health benefits without side effects. In this regard, preventive effect offered by DK juice against the development of IGT, and imbalance in antioxidant defense induced due to sugar consumption may become cost effective alternative natural substitute at the place of sugar-sweetened beverages. Further research is required, however, to translate health promoting effect of DK juice in clinical conditions. To the best of our knowledge, this is the first scientific evaluation and health promoting effect of DK juice, which may be helpful in preventing development of dysglycemia and oxidative stress induced by consumption of sugar-sweetened beverages.
| Conclusions|| |
The whole fruit juice of DK may become cost effective, affordable health beverage in extenuating ill-health effects of sugar consumption. This is the first report identifying DK juice in preventing development of dysglycemia, dyslipidemia, and oxidative stress induced due to chronic sucrose feeding in rats.
The authors thank D'CSIR-IICT for constant encouragements during this study. This research was supported in part by CSIR; New Delhi supported research grants in SMiLE (CSC-0111) and NAPAHA (CSC-0130) projects. DAK thanks CSIR-IICT for SPF under SMiLE (CSC-0111) project.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Johnson RJ, Segal MS, Sautin Y, Nakagawa T, Feig DI, Kang DH, et al.
Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease. Am J Clin Nutr 2007;86:899-906.
Bray GA, Popkin BM. Dietary sugar and body weight: Have we reached a crisis in the epidemic of obesity and diabetes? health be damned! Pour on the sugar. Diabetes Care 2014;37:950-6.
Schmidt LA. New unsweetened truths about sugar. JAMA Intern Med 2014;174:525-6.
Weeratunga P, Jayasinghe S, Perera Y, Jayasena G, Jayasinghe S. Per capita sugar consumption and prevalence of diabetes mellitus – Global and regional associations. BMC Public Health 2014;14:186.
Limb M. Halve sugar intake to 5% of daily calories, says advisory group. BMJ 2014; 348:g4310.
Imai S, Kajiyama S. What to eat first and how to eat to reduce amplitude of glycemic excursions. J Life Sci Res 2014;12:3-7.
Imai S, Fukui M, Kajiyama S. Effect of eating vegetables before carbohydrates on glucose excursions in patients with type 2 diabetes. J Clin Biochem Nutr 2014;54:7-11.
Tiwari AK. Revisiting "Vegetables" to combat modern epidemic of imbalanced glucose homeostasis. Pharmacogn Mag 2014;10:S207-13.
Parthasarathy VA, Sambandam CN. Taxonomic position of dosakaya (Cucumis
sp.) – The acid melon of India. Cucurbit Genet Coop Rep 1980;3:35.
Tiwari AK, Reddy KS, Radhakrishnan J, Kumar DA, Zehra A, Agawane SB, et al.
Influence of antioxidant rich fresh vegetable juices on starch induced postprandial hyperglycemia in rats. Food Funct 2011;2:521-8.
Tiwari AK, Kumar AD, Sweeya PS, Abhinay KM, Hanumantha AC, Lavanya V, et al.
Protein – Tyrosine phosphatase 1 β inhibitory activity potential in vegetable juice. Pharmacologia 2013a; 4:311-3.
Tiwari AK, Kumar DA, Sweeya PS, Chauhan HA, Lavanya V, Sireesha K, et al.
Vegetables' juice influences polyol pathway by multiple mechanisms in favour of reducing development of oxidative stress and resultant diabetic complications. Pharmacogn Mag 2014;10:S383-91.
Tiwari AK, Anusha I, Sumangali M, Kumar DA, Madhusudana K, Agawane SB. Preventive and therapeutic efficacies of Benincasa hispida and Sechium edule
fruit's juice on sweet-beverages induced impaired glucose tolerance and oxidative stress. Pharmacologia 2013b; 4:197-207.
Tiwari AK, Kumbhare RM, Agawane SB, Ali AZ, Kumar KV. Reduction in post-prandial hyperglycemic excursion through alpha-glucosidase inhibition by beta-acetamido carbonyl compounds. Bioorg Med Chem Lett 2008;18:4130-2.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.
Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 1975;250:5475-80.
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
Thornalley PJ. Modification of the glyoxalase system in human red blood cells by glucose in vitro
. Biochem J 1988;254:751-5.
Wills ED. Lipid peroxide formation in microsomes. Relationship of hydroxylation to lipid peroxide formation. Biochem J 1969;113:333-41.
Mitsuhashi T, Nakayama H, Itoh T, Kuwajima S, Aoki S, Atsumi T, et al.
Immunochemical detection of advanced glycation end products in renal cortex from STZ-induced diabetic rat. Diabetes 1993;42:826-32.
Abdul-Ghani MA, Lyssenko V, Tuomi T, Defronzo RA, Groop L. The shape of plasma glucose concentration curve during OGTT predicts future risk of type 2 diabetes. Diabetes Metab Res Rev 2010;26:280-6.
Moutzouri E, Tsimihodimos V, Rizos E, Elisaf M. Prediabetes: To treat or not to treat? Eur J Pharmacol 2011;672:9-19.
Poli A. Keep your postprandial curve flat. Nutrafoods 2013;12:33.
Tabák AG, Herder C, Rathmann W, Brunner EJ, Kivimäki M. Prediabetes: A high-risk state for diabetes development. Lancet 2012;379:2279-90.
Monnier L, Mas E, Ginet C, Michel F, Villon L, Cristol JP, et al.
Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes. JAMA 2006;295:1681-7.
Slama G, Elgrably F, Sola A, Mbemba J, Larger E. Postprandial glycaemia: A plea for the frequent use of delta postprandial glycaemia in the treatment of diabetic patients. Diabetes Metab 2006;32:187-92.
O'Keefe JH, Gheewala NM, O'Keefe JO. Dietary strategies for improving post-prandial glucose, lipids, inflammation, and cardiovascular health. J Am Coll Cardiol 2008;51:249-55.
Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res 2010;107:1058-70.
Lushchak VI. Glutathione homeostasis and functions: Potential targets for medical interventions. J Amino Acids 2012;2012:736837.
Ulusu NN, Acan NL, Turan B, Tezcan EF. The effect of selenium on glutathione redox cycle enzymes of some rabbit tissues. Trace Elem Electrolytes 2000;17:25-9.
Kalapos MP. Methylglyoxal in living organisms: Chemistry, biochemistry, toxicology and biological implications. Toxicol Lett 1999;110:145-75.
Perron NR, Brumaghim JL. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys 2009;53:75-100.
Ningappa MB, Srinivas L. Purification and characterization of approximately 35 kDa antioxidant protein from curry leaves (Murraya koenigii
L.). Toxicol In Vitro
Tiwari AK, Kumar PM, Anand KD, Agawane SB, Madhusudana K, Zehra A. Ayurvedic dietary formulations and postprandial glycemia in rats. Int Food Res J 2012;19:765-73.
| Authors|| |
Dr. Ashok K. Tiwari, obtained his Ph. D. degree in 1992 from Institute of Medical Sciences, Banaras Hindu University, Varanasi. Currently, he is positioned as Senior Principal Scientist at the Medicinal Chemistry & Pharmacology Division of CSIR-Indian Institute of Chemical Technology, Hyderabad (India). He is also Professor of Biological Sciences in Academy of Scientific and Innovative Research (AcSIR). Dr. Tiwari is working on various aspects of life-style related metabolic disorders and engaged in finding preventive as well as therapeutic solutions from indigenous resources.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]