Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2015  |  Volume : 11  |  Issue : 43  |  Page : 665-673

Effect of modified Bo-yang-Hwan-o-Tang, a polyherbal medicine on the hippocampal neuronal damage in a rat model of global ischemia


1 Department of Herbology, College of Korean Medicine, Gyeongju 780-714, Republic of Korea
2 Department of Herbology, College of Korean Medicine; Korean Medicine R&D Center, Dongguk University, Gyeongju 780-714, Republic of Korea

Correspondence Address:
Yong-Ki Park
Department of Herbology, College of Korean Medicine; Korean Medicine R&D Center, Dongguk University, Gyeongju 780-714, Republic of Korea

Login to access the Email id

Source of Support: Nil, Conflict of Interest: None declared.


DOI: 10.4103/0973-1296.160445

Rights and Permissions

Background: Chronic cerebral hypoperfusion has been well characterized as a common pathological status contributing to vascular dementia (VD). In this study, the neuroprotective effect of modified Bo yang Hwan O Tang (mBHT), a polyherbal medicine for ischemic stroke, was investigated in a rat model for global ischemia. Materials and Methods: Global ischemia model was prepared in Sprague Dawley rats by the permanent occlusion of bilateral common carotid arteries (two vessel occlusion [2VO]) induced chronic cerebral hypoperfusion. mBHT at doses of 250 and 500 mg/kg was orally administrated for 4 weeks once a day, 24 h after 2VO. Histopathological change of the hippocampal region was observed by hematoxylin and eosin, Nissl, and Fluoro Jade B staining and immunohistochemistry with anti glial fibrillary acidic protein and anti neuronal nuclei antibodies. The expression of Bax, Bcl 2, and caspase 3 was investigated in the hippocampus by Western blot. The nuclear factor kappa B (NF κB) expression was also analyzed in hippocampal CA1 region using immunofluorescence staining. Results: The administration of mBHT at doses of 250 and 500 mg/kg significantly inhibited chronic cerebral hypoperfusion induced neuronal damage and astroglial activation in the hippocampal CA1 region in 2VO rats. mBHT increased the NF κB expression in the CA1 neuronal cells but decreased in activated astrocytes. In addition, mBHT significantly decreased the hippocampal expression of Bax and caspase 3 and increased the Bcl 2 expression in 2VO rats. Conclusions: Our data indicate that mBHT has a neuroprotective property in VD induced by chronic cerebral hypoperfusion through inhibiting the hippocampal neuronal damage and astrogliosis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1877    
    Printed20    
    Emailed0    
    PDF Downloaded17    
    Comments [Add]    

Recommend this journal