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ORIGINAL ARTICLE
Year : 2015  |  Volume : 11  |  Issue : 43  |  Page : 486-501

Profiling of components of rhizoma et radix polygoni cuspidati by high-performance liquid chromatography with ultraviolet diode-array detector and ion trap/time-of-flight mass spectrometric detection


1 Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Pharmaceutical University, China
2 Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Pharmaceutical University; Department of Pharmaceutical Analysis, China Pharmaceutical University, Tongjia Lane 24, Nanjing 210009, China

Correspondence Address:
Rui Song
Department of Pharmaceutical Analysis, China Pharmaceutical University, Tongjia Lane 24, Nanjing 210009
China
Zunjian Zhang
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Tongjia Lane 24, Nanjing 210009
China
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Source of Support: Natural Science Foundation of China (No. 81403314), Natural Research Foundation of Jiangsu Province (No. BK2012349), Fundamental Research Funds for the Central Universities (JKPZ2013008) and the Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (No. MKLDP2013MS06)., Conflict of Interest: None declared.


DOI: 10.4103/0973-1296.160455

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Introduction: Rhizoma et Radix Polygoni Cuspidati (Huzhang in Chinese, HZ) is a traditional medicinal plant in China. Many of the components of HZ have been proved to be bioactive while it is difficult to conduct a comprehensive chemical profiling of HZ as a consequence of the absence of efficient separation system and sensitive detective means. We developed a simple and effective method for comprehensive characterization of constituents in HZ. Objective: To develop a simple and effective method to characterize the components in HZ and provide useful information for subsequent metabolic studies of HZ. Materials and Methods: The components in HZ aqueous extract were characterized by using high performance liquid chromatography with UV diode-array detector (HPLC-DAD) and ion trap/time-of-flight mass spectrometric detection (HPLC-IT/TOF). Stilbenes, anthraquinones, gallates and tannins, naphthalenes and some other compounds were identified and confirmed by diagnostic fragment ions with accurate mass measurements, characteristic fragmentation pathways and relevant published literatures. Results: Among the 238 constituents detected in HZ, a total number of 74 constituents were identified unambiguously or tentatively, including 29 compounds reported for the first time in HZ. Conclusion: The identification and structure elucidation of these chemicals provided essential data for quality control and further in vivo metabolic studies of HZ.Key words: Polygonum cuspidatum, HPLC-DAD, HPLC-IT/TOF, qualitative analysis.


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