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ORIGINAL ARTICLE
Year : 2015  |  Volume : 11  |  Issue : 43  |  Page : 477-485

A direct protein kinase B-targeted anti inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris


1 Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea
2 Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseoung 369 873, Korea
3 Department of Pharmacy, School of Pharmacy, Sungkyunkwan University, Suwon 440 746, Korea

Correspondence Address:
Hyung Jun Noh
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseoung 369 873
Korea
Jae Youl Cho
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440 746
Korea
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Source of Support: Nil, Conflict of Interest: None declared.


DOI: 10.4103/0973-1296.160454

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Background: Cordyceps militaris is one of well-known medicinal mushrooms with anti-inflammatory, anti-cancer, anti-diabetic, and anti-obesity activities. Objective: The objective of the following study is to isolate chemical components from the ethanol extract (Cm-EE) from Cordyceps militaris and to evaluate their anti-inflammatory activities. Materials and Methods: Column chromatographic separation was performed and anti-inflammatory roles of these compounds were also examined by using NO production and protein kinase B (AKT) activity assays. Results: From Cm-EE, 13 constituents, including trehalose (1), cordycepin (2), 6-hydroxyethyladenosine (3), nicotinic amide (4), butyric acid (5), β-dimorphecolic acid (6), α-dimorphecolic acid (7), palmitic acid (8), linoleic acid (9), cordycepeptide A (10), 4-(2-hydroxy-3-((9E,12E)-octadeca-9,12-dienoyloxy)propoxy)-2-(trimethylammonio)butanoate (11), 4-(2-hydroxy-3-(palmitoyloxy)propoxy)-2-(trimethylammonio)butanoate (12), and linoleic acid methyl ester (13) were isolated. Of these components, compound 2 displayed a significant inhibitory effect on NO production in lipopolysaccharide (LPS)-activated RAW264.7 cells. Furthermore, this compound strongly and directly suppressed the kinase activity of AKT, an essential signalling enzyme in LPS-induced NO production, by interacting with its ATP binding site. Conclusion: C. militaris could have anti-inflammatory activity mediated by cordycepin-induced suppression of AKT.


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