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ORIGINAL ARTICLE
Year : 2015  |  Volume : 11  |  Issue : 42  |  Page : 360-367

Simultaneous quantification of six alkaloid components from commercial stemonae radix by solid phase extraction-high-performance liquid chromatography coupled with evaporative light scattering detector


1 College of Pharmacy, Jinan University, Guangzhou, 510632, China
2 School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
3 College of Pharmacy, Jinan University, Guangzhou, 510632; Shenzhen Engineering Laboratory of Lingnan Herbal Resource Development and Application, Shenzhen Institute for Drug Control, Shenzhen 518057, China

Correspondence Address:
Ren-Wang Jiang
College of Pharmacy, Jinan University, Guangzhou
China
Zhi-Guo Ma
College of Pharmacy, Jinan University, Guangzhou
China
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Source of Support: This project was partially supported by the Guangdong Major Scientific and Technological Special Project for New Drug Development (R.W.J.), Conflict of Interest: None


DOI: 10.4103/0973-1296.153090

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Background: Stemonae radix has been applied in traditional Chinese medicine for centuries. Alkaloids are the main active ingredient in stemonae radix, so their composition and concentration levels are directly linked to clinic effects. Objective: The objective was to develop an analytical method with multiple markers for quality survey of commercial stemonae radix. Materials and Methods: A method for simultaneous determination of six compounds in commercial stemonae radix was performed using solid-phase extraction and high-performance liquid chromatography coupled with evaporative light scattering detector. The separation was carried out on an Agilent TC-C18 column with 0.1% acetonitrile solution of triethylamine aqueous solution and acetonitrile as the mobile phase under gradient elution within 70 min. The hierarchical clustering analysis (HCA) was successfully used to classify the samples in accordance with their chemical constituents. Results: Linearity (R 2 > 0.9990), intra- and inter-day precision (relative standard deviations <4%), limit of detection (0.011-0.086 μg/mL), limit of quantification (0.033-0.259 μg/mL) of the six alkaloids were determined, and the recoveries were between 96.6% and 103.7%. The method was successfully applied to analysis 36 batches of commercial stemonae radix. All the samples could be classified into five clusters by HCA. Conclusion: This article provides an accurate and simple analytical method for quality survey of commercial stemonae radix. Because of the significant chemical variations, careful selection of Stemona sources with obvious antitussive value but devoid of croomine followed by good agricultural practice and good manufacturing practice process is suggested.


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