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ORIGINAL ARTICLE
Year : 2015  |  Volume : 11  |  Issue : 41  |  Page : 176-179

Chromatographic method for determination of the free amino acid content of chamomile flowers


Analytical and Testing Center, School of Pharmacy, Xinjiang Medical University, Urumqi, Xiangjiang, China

Correspondence Address:
Xinxia Li
Analytical and Testing Center, School of Pharmacy, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, Xinjiang - 830 011
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.149735

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Objective: To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. Materials and Methods: Derivatization with reconstituted AQC was used to prepare the samples and standards for injection into the chromatography column. The peaks were analyzed by fluorescence detection (λ excitation, 250 nm; λ emission, 395 nm. Results: Alanine, proline, and leucine were the most abundant amino acids, whereas tyrosine and methionine were the least abundant. The linearity of the method was found to be good with amino acid concentrations of 0.012-0.36 μM. The precision was 0.05-1.36%; average recovery, 91.12-129.41%; and limit of detection, 0.006-0.058 μM. Conclusion: The method is reliable for determining the free amino acid content of different types of chamomile flowers.


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