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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 40  |  Page : 415-421

Application of microscopy technique and high performance liquid chromatography for quality assessment of Polygonum multiflorum Thunb. (Heshouwu)


1 School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian; School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2 School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
3 School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China

Correspondence Address:
Tingguo Kang
77# Life One Road, DD Port, Development Area, Dalian 116600
China
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Source of Support: This project was supported by the National Natural Science Foundation of China (NO. 81173499), Conflict of Interest: None


DOI: 10.4103/0973-1296.141788

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Background: The technique of microscopy has been applied for identification of Chinese materia medica (CMM) since decades. However, very few scientific publications report the combination of conventional microscopy and high performance liquid chromatography (HPLC) techniques for further application to quality assessment of CMM. Objective: The objective of this study is to analyze the quality of the dried root tuber of Polygonum multiflorum Thunb. (Heshouwu) and to establish the relationships between 2,3,5,4'- tetrahydroxystilbene-2-O-β-glucoside, combined anthraquinone (CAQ) and quantity of clusters of calcium oxalate. Materials and Methods: In this study, microscopy and HPLC techniques were applied to assess the quality of P. multiflorum Thunb., and SPSS software was used to establish the relationship between microscopic characteristics and chemical components. Results: The results showed close and direct correlations between the quantity of clusters of calcium oxalate in P. multiflorum Thunb. and the contents of 2,3,5,4'- tetrahydroxystilbene-2-O-β-glucoside and CAQ. From these results, it can be deduced that Polygoni Multiflori Radix with a higher quantity of clusters of calcium oxalate should be of better quality. Conclusion: The established method can be helpful for evaluating the quality of CMM based upon the identification and quantitation of chemical and ergastic substance of cells.


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