Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 40  |  Page : 402-409

Effects of Astragaloside IV combined with the active components of Panax notoginseng on oxidative stress injury and nuclear factor-erythroid 2-related factor 2/heme oxygenase-1 signaling pathway after cerebral ischemia-reperfusion in mice


1 Molecular Pathology Laboratory, Hunan University of Chinese Medicine; Key Laboratory of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardiocerebral Diseases, Hunan, China
2 Key Laboratory of Hunan Universities for Cell Biology and Molecular Techniques Changsha 410208, Hunan, China
3 Molecular Pathology Laboratory, Hunan University of Chinese Medicine, Hunan, China

Correspondence Address:
Chang-Qing Deng
Molecular Pathology Laboratory, Hunan University of Chinese Medicine, Changsha 410208
China
Login to access the Email id

Source of Support: National Natural Science Foundation of China (No. 81102557); Doctoral Program Foundation of Higher Education of China (No. 20104323110001); Aid Project for Innovation Platform Open Fund of Hunan province university (No. 11K050); Key Project of Administration of traditional Chinese Medicine of Hunan province (No. 201301); General Project of Education Bureau of Hunan Province (No. 11C0963); Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province for gRelated research on prevention and treatment of cardio.cerebrovascular diseases with integrated traditional Chinese and Western Medicineh, Hunan Province Natural Science Innovation Group for gFundamental research of Chinese medicine on prevention and treatment of cardio.cerebrovascular diseasesh, Conflict of Interest: None


DOI: 10.4103/0973-1296.141765

Rights and Permissions

Background: Astragalus and Panax notoginseng are traditional Chinese Medicines used for the treatments of ischemic cerebrovascular disease, being often combined together in China and achieving a good effect. Objective: The objective of this study is to investigate the effects of astragaloside-IV (AST-IV) (the effective component of Astragalus) combined with ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1 (the effective components of P. notoginseng) on oxidative stress injury after cerebral ischemia-reperfusion in mice, and to explore the mechanisms through nuclear factor-erythroid 2-related factor-2/heme oxygenase-1 (Nrf2/HO-1) signaling pathway. Materials and Methods: C57BL/6 mice were randomly grouped after treated for 3 days, the model of cerebral ischemia-reperfusion injury was established, and the brain tissues were detected. Results: AST-IV combined with ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1 could increase significantly the survival rate of nerve cell; decrease the contents of malondialdehyde, nitric oxide, increase the activity of superoxide dismutase and the level of glutathione; Nrf2 was down-regulated in the cytoplasm while up-regulated in nucleus, nuclear translocation rate raised as well as HO-1 messenger ribonucleic acid and protein expressions increased. The effects of four active components combination were better than those of the active components alone. Conclusion: Active components of Astragalus and P. notoginseng had the effects against cerebral ischemia-reperfusion injury, which were related to the antioxidative stress after cerebral ischemia-reperfusion. AST-IV combined with ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1 could strengthen the antagonism effects on ischemia-reperfusion and oxidative stress injury, the mechanism underlying might be associated with jointly activating Nrf2/HO-1 signaling pathway after cerebral ischemia-reperfusion.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1737    
    Printed55    
    Emailed0    
    PDF Downloaded19    
    Comments [Add]    
    Cited by others 8    

Recommend this journal