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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 40  |  Page : 385-390

Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by dna - barcoding technique


1 School of Pharmaceutical Sciences & Yunnan Key Laboratory of Pharmacology for Nature Products, Kunming Medical University,Kunming 650500, China
2 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
3 Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China
4 Key Laboratory of Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China

Correspondence Address:
Zhenwen Liu
Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132#, Kunming - 650 201
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.141754

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Background: Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region. Objective: To authenticate P. praeruptorum from its substitutes and adulterants. Materials and Methods: The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimura's 2-parameter method was also calculated. Results: The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree. Conclusion: The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.


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