Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 39  |  Page : 630-638

The Pavonia xanthogloea (Ekman, Malvaceae): Phenolic compounds quantification, anti-oxidant and cytotoxic effect on human lymphocytes cells


1 Department of Physiology, Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, Brazil
2 Department of Chemistry, Federal University of Santa Maria, Santa Maria, Brazil
3 Department of Chemistry, Graduate Program in Biochemistry and Toxicology, Science Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil
4 Department of Morphology, Laboratory of Biogenomics, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil
5 Department of Physiology, Graduate Program in Pharmacology; Department of Morphology, Laboratory of Biogenomics, Health Sciences Center; Department of Chemistry, Graduate Program in Biochemistry and Toxicology, Science Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil

Correspondence Address:
Ivana Beatrice Mānica da Cruz
Av Roraima 1000, Building 19, Laboratory of Biogenomic, Federal University of Santa Maria, Santa Maria-RS, Zip Code 97105-900
Brazil
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.139804

Rights and Permissions

Introduction: Pavonia xanthogloea is traditionally used as an antimicrobial and anti-tumour medicine in Southern Brazilian region. However, investigations about this species are still incipient. Hypothesis Tested: The study postulated that P. xanthologea specie present some phenolic compound and present some biological properties as anti-oxidant and cytoprotective effect against oxidative stress. Materials and Methods: The content of eight phenolic molecules in the crude ethanolic extract of the aerial part of P. xanthogloea and its five fractions (hexane, dichloromethane, ethyl-acetate, n-butanol, and water) was determined by heterotrophic plate count method. The anti-oxidant capacity of the extract and the fractions was determined by 1,1-diphenyl-2-picryl-hydrazyl assay. The potential anti-oxidant and cytoprotective effect was also analyzed in human lymphocyte culture treated with extract/fractions at different concentrations with and without oxidative stress generated by hydrogen peroxide (H 2 O 2 ) and sodium nitroprusside (SNP) exposition. Results: Tiliroside was the molecule detected in all extract. Water and ethyl-acetate fractions showed the highest radical-scavenging activity. The crude extract, hexane, water, and n-butanol reversed the higher reactive oxygen specie levels generated by H 2 O 2 and SNP to levels similar to those observed in the control group. In addition, crude extract, hexane, ethyl-acetate and n-butanol did not caused cytotoxicity, whereas water fraction was cytotoxic at higher concentration tested here (300 μg/mL). The cytotoxicity reversion caused by SNP exposition was concentration-dependent of the extract and fractions. However, dichloromethane fraction increased cell mortality in all concentrations investigated and was not able to decrease cell death in the lymphocytes exposed to SNP. Conclusion: The results suggest potential medicine use of this species.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1180    
    Printed56    
    Emailed0    
    PDF Downloaded15    
    Comments [Add]    

Recommend this journal