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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 38  |  Page : 425-429

Effect of ultrasound on the isoflavonoid production in Genista tinctoria L. suspension cultures


1 Department of Pharmacognosy, Faculty of Pharmacy in Hradec Kralove, Charles University Prague, Heyrovskeho 1203, 500 05
2 Department of Biology, University of Hradec Kralove, Faculty of Natural Sciences, Rokitanskeho 62 500 03 Hradec Kralove, Czech Republic

Correspondence Address:
Lenka Tumova
Department of Pharmacognosy, Faculty of Pharmacy in Hradec Kralove, Charles University Prague, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.133297

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Background: Application of ultrasound (US) to biotechnology is relatively new but several processes that take place in the presence of cells or enzymes are activated by ultrasonic waves. Genista tinctoria L. (Fabaceae) is rich on various kind of flavonoids, including isoflavones with valuable estrogenic activity. Objective: This study verified use of low-energy US elicitor to enhance secondary metabolite production in plant cell cultures. Materials and Methods: Suspension cultures of G. tinctoria cells was exposed to low-power US (with fixed frequency 35 kHz and power level 0.1 mW/cm 3 ) for period 1-5 min. Results: The US exposure significantly stimulated genistin content (0.8 mg/g DW) after 3 min of US treatment (sampled after 72 h). The highest daidzein level (1.4 mg/g DW) was reached after US irradiation for 5 min and 168 h sampling. Conclusion: The achieved results suggest that US can act as a potent abiotic elicitor to induce the defense responses of plant cells and to stimulate secondary metabolite production in plant cell cultures.


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