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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 37  |  Page : 141-146

Ultrasound-assisted extraction of total flavonoids from Aconitum gymnandrum


1 Department of Pharmacy, The First Affiliated Hospital, Chengdu Medical College, Middle Section, Baoguang Road, Xindu Region, Chengdu 610 500, China
2 Department of Chinese Traditional Herbal, Agronomy College, Sichuan Agricultural University, Huiming Road, Wenjiang Region, Chengdu 611 130, China

Correspondence Address:
Feng Gao
Department of Chinese Traditional Herbal, Agronomy College, Sichuan Agricultural University, No. 211, Huiming Road, Wenjiang Region, Chengdu 611130
China
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Source of Support: Grant from the NSFC (No. 81001383), the Doctoral Foundation of Ministry of Education of China (No. 20105103120009), Applied Basic Research Programs of Science and Technology Department of Sichuan Province (No. 2012JY0035) and the research fund of Chengdu Medical College (No. CYZ11-021), Conflict of Interest: None


DOI: 10.4103/0973-1296.127364

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Background: Aconitum gymnandrum is a Chinese traditional herb used as carminative and analgesic. In this study, A. gymnandrum was used as an experimental matrix. Materials and Methods: Optimized ultrasonic extraction technology of total flavonoids from the A. gymnandrum Maxim was studied by using the methodology of single factor and orthogonal design to study the effects of operation conditions, such as ethanol content, ultrasonic wave power, temperature, ultrasonic wave radiation time, and the ratio of sample weight to solvent volume. Result: Through the orthogonal experiment, the optimal extraction conditions were determined as follows: Ultrasonic power 100 W, ultrasonic temperature 45°C, 60% ethyl alcohol, extraction time 30 min, and solid-liquid ratio 1:20. Conclusion: Under the optimum parameters, the extraction ratio of total flavonoids from the A. gymnandrum Maxim is about 1.278%.


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