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ORIGINAL ARTICLE
Year : 2013  |  Volume : 9  |  Issue : 35  |  Page : 210-215

Response surface modeling and optimization of ultrasound-assisted extraction of three flavonoids from tartary buckwheat (Fagopyrum tataricum)


College of Biotechnology Industry, Chengdu University, Chengluo Road, Longquanyi District, Chengdu 610106, Sichuan, China

Correspondence Address:
Gang Zhao
College of Biotechnology Industry, Chengdu University, Chengluo Road, Longquanyi District, Chengdu 610106, Sichuan
China
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Source of Support: This research was financially supported by the National Spark Program of China (2010GA812002), a fund earmarked for the China Agriculture Research System (CARS-08-B-3, CARS-08-D-3), and by the Administration Foundation of Traditional Chinese Medicine of Sichuan., Conflict of Interest: None


DOI: 10.4103/0973-1296.113266

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Background: Buckwheat (Fagopyrum spp., Polygonaceae) is a widely planted food crop. Flavonoids, including quercetin, rutin, and kaempferol, are the main bioactive components in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn). From the nutriological and pharmacological perspectives, flavonoids have great value in controlling blood glucose and blood pressure levels, and they also have antioxidant properties. Objective: To optimize the conditions for extraction of quercetin, rutin, and kaempferol from F. tataricum. Materials and Methods: A combination of ultrasound-assisted extraction (UAE) and response surface methodology (RSM) was used for flavonoid extraction and yield assessment. The RSM was based on a three-level, three-variable Box-Behnken design. Results: Flavonoids were optimally extracted from F. tataricum by using 72% methanol, at 60°C, for 21 minutes. Under these conditions, the obtained extraction yield of the total flavonoids was 3.94%. Conclusion : The results indicated that the UAE method was effective for extraction of flavonoids from tartary buckwheat.


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