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ORIGINAL ARTICLE
Year : 2013  |  Volume : 9  |  Issue : 35  |  Page : 202-209

Comparison of the protective effects of ferulic acid and its drug - containing plasma on primary cultured neonatal rat cardiomyocytes with hypoxia/reoxygenation injury


1 Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, China
2 Department of Pharmacy, Dalian Medical University, Dalian, Liaoning, P.R., China

Correspondence Address:
Xian-sheng Meng
Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning, P.R.
China
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Source of Support: This work was "Chinese Eleventh five-year plan " and " The special issues of major national science and technology”,"Component of Chinese herbal medicines sub-platform " (item number: 2010ZX09401-304- 105C), " Combination components the new drug Salvia Xinmaitong capsule developement” (item number: 2010ZX09401-304-515) and it was supported by a grant from National Key New Drug Creation Special Management Office and and Liaoning Pharmaceutical Co., Ltd. P.R. China. The authors would like to thank the authorities in the Department of Pharmacy, Liaoning University of Traditional Chinese Medicine and Dianlian Medical University, P.R. China, for their support., Conflict of Interest: None


DOI: 10.4103/0973-1296.113264

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Backgroud: To simulate the ischemia-reperfusion injury in vivo, hypoxia/reoxygenation injury model was established in vitro and primary cultured neonatal rat cardiomyocytes were underwent hypoxia with hydrosulfite (Na 2 S 2 O 4 ) for 1 h followed by 1 h reoxygenation. Materials and Methods: Determination the cell viability by MTT colorimetric assay. We use kit to detect the activity of lactate dehydrogenase (LDH), Na + -K + -ATPase and Ca 2+ -ATPase. Do research on the effect which ferulic acid and its drug-containing plasma have to self-discipline, conductivity, action potential duration and other electrophysiological phenomena of myocardial cells by direct observation using a microscope and recording method of intracellular action potential. Results: The experimental datum showed that both can reduce the damage hydrosulfite to myocardial cell damage and improve myocardial viability, reduce the amount of LDH leak, increase activity of Na + -K + -ATPase, Ca 2+ -ATPase, and increase APA (Action potential amplitude), Vmax (Maximum rate of depolarization) and MPD (Maximum potential diastolic). Conclusion: Taken together, therefore, we can get the conclusion that ferulic acid drug-containing plasma has better protective effect injured myocardial cell than ferulic acid.


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