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ORIGINAL ARTICLE
Year : 2013  |  Volume : 9  |  Issue : 35  |  Page : 192-195

Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium


1 Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei, China
2 Department of Pharmacy, HeBei North University, Zhangjiakou, 075000, China
3 Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei; HeBei University of Science and Technology, Shijiazhuang, 050018, China

Correspondence Address:
Dan-Shen Zhang
HeBei Medical University, HeBei University of Science and Technology, Shijiazhuang
China
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Source of Support: financially supported by the Hebei administration of traditional Chinese medicine project (No.2010046), the project of Hebei Education Department (No.Z2011304), and Natural Science Foundation of Hebei Province (H2012405016), Conflict of Interest: None


DOI: 10.4103/0973-1296.113260

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Background: Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. Materials and Methods: The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L 9 (3 4 )) was then constructed to achieve the best extraction conditions. Results: Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C. Conclusion: Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.


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