Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2013  |  Volume : 9  |  Issue : 34  |  Page : 155-161

Simultaneous determination of eleven characteristic lignans in Schisandra chinensis by high-performance liquid chromatography


1 College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046, China
2 College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046; Jiangsu Province Key Research Laboratory of Chinese Medicine Processing, Nanjing, 210046, China
3 Jiangsu Province Key Laboratory of Acupuncture of Nanjing University of Chinese Medicine, Nanjing, 210046, China
4 Jiangsu Province Key Research Laboratory of Chinese Medicine Processing, Nanjing, 210046, China

Correspondence Address:
Tulin Lu
College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046
China
Login to access the Email id

Source of Support: Major Program of State Commission of Science Technology of China O.2009ZX09308-004), Educational Commission of Jiangsu Province of China (NO.09KJA360001), and Key Laboratory of Acupuncture of Jiangsu Province of China (NO.KJA200906)., Conflict of Interest: None


DOI: 10.4103/0973-1296.111280

Rights and Permissions

Background: Schisandra chinensis, one of the well-known traditional Chinese herbal medicines, is derived from the dry ripe fruits of Schisandra chinensis (Turcz.) Baill. according to the 9th China Pharmacopeia. Lignans are the main components isolated from extracts of S. chinensis and their content varies depending on where S. chinensis was collected. We have established a qualitative and quantitative method based on the bioactive lignans for control of the quality of S. chinensis from different sources. Materials and Methods: To develop a high-performance liquid chromatography method, an Elite ODS C18 column (250 mm Χ 4.6 mm, 5μm) at a column temperature of 30°C and flow rate of 1.0ml/min using acetonitrile (A) and water (B) as the mobile phase with a linear gradient and the peaks were monitored at 217 nm. Results: All calibration curves showed good linearity (r ≥ 0.9995) within test ranges. This method showed good repeatability for the quantification of these eleven components in S. chinensis with intra- and inter-day relative standard deviations less than 0.43% and 1.21%, respectively. In the recovery test, results of accuracy ranged from 99.51% to 101.31% with RSD values less than 2.68%, Conclusion: The validated method can be successfully applied to quantify the eleven investigated components in 22 samples of S. chinensis from different sources.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2439    
    Printed78    
    Emailed0    
    PDF Downloaded19    
    Comments [Add]    
    Cited by others 2    

Recommend this journal