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ORIGINAL ARTICLE
Year : 2012  |  Volume : 8  |  Issue : 31  |  Page : 209-214

Antimicrobial activity of Marcetia DC species (Melastomataceae) and analysis of its flavonoids by reverse phase-high performance liquid chromatography coupled-diode array detector


1 Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil
2 Federal Institute for Education, Science and Technology of Pernambuco - Campus Barreiras, Barreiras, Pernambuco, Brazil
3 Department of Biological Sciences, State University of Feira de Santana, Feira de Santana, Bahia, Brazil
4 Institute of Multidisciplinary Health - Campus Anísio Teixeira, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil
5 Microbiology Laboratory of Agribusiness, Department of Biological Sciences, State University of Santa Cruz, Ilhéus, Bahia, Brazil

Correspondence Address:
Alexsandro Branco
Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Av. Transnordestina, s/nº, Bairro Novo Horizonte, 44.036-900, Feira de Santana, Bahia
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.99286

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Background: Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil), and most are endemic to the highlands of the Chapada Diamantina (Bahia). Among the species, only M. taxifolia (A.St.-Hil.) DC. populates Brazil (state of Roraima to Paranα) and also Venezuela, Colombia, and Guyana. Objective: This work evaluated the antimicrobial activity of hexane, ethyl acetate, and methanol extracts of three species of Marcetia (Marcetia canescens Naud., M. macrophylla Wurdack, and M. taxifolia A.StHil) against several microorganism. In addition, the flavonoids were analyzed in extracts by HPLC-DAD. Materials and methods: The tests were made using Gram-positive (three strains of Staphylococcus aureus) and Gram-negative (two strains of Escherichia coli, a strain of Pseudomonas aeruginosa and another of Salmonella choleraesius) bacteria resistant and nonresistant to antibiotics and yeasts (two strains of Candida albicans and one of C. parapsilosis) by the disk diffusion method. Solid-phase extraction (SPE) was performed on the above extracts to isolate flavonoids, which were subsequently analyzed by high performance liquid chromatography coupled diode array detector (HPLC-DAD). Results: Results showed that extracts inhibited the Gram-positive bacteria and yeast. The hexane extracts possessed the lowest activity, while the ethyl acetate and methanolic extracts were more active. Conclusion: Marcetia taxifolia was more effective (active against 10 microorganisms studied), and only its methanol extract inhibited Gram-negative bacteria (P. aeruginosa and S. choleraesius). SPE and HPLC-DAD analysis showed that M. canescens and M. macrophylla contain glycosylated flavonoids, while the majority of extracts from M. taxifolia were aglycone flavonoids.


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